{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,14]],"date-time":"2025-05-14T02:06:29Z","timestamp":1747188389265,"version":"3.40.5"},"reference-count":12,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2021,5,27]],"date-time":"2021-05-27T00:00:00Z","timestamp":1622073600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Transfusion"],"published-print":{"date-parts":[[2021,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>The PGD<jats:italic>prime<\/jats:italic>\u00ae test was updated to enable <jats:italic>Acinetobacter<\/jats:italic> spp. detection to respond to morbidity and mortality events in 2018 and 2020 involving platelets contaminated with <jats:italic>Acinetobacter\u2010calcoaceticus\u2010baumannii<\/jats:italic> complex (<jats:italic>ACBC<\/jats:italic>). In one morbidity event, the first\u2010generation PGD test failed to detect <jats:italic>ACBC<\/jats:italic>. In two other reported events, pathogen\u2010reduced (PR) platelets contaminated with <jats:italic>ACBC<\/jats:italic> and other bacteria led to patient morbidity and one death.<\/jats:p><\/jats:sec><jats:sec><jats:title>Study Design and Methods<\/jats:title><jats:p>A polyclonal antibody to <jats:italic>Acinetobacter<\/jats:italic> was integrated in the test device and evaluated for detection of <jats:italic>Acinetobacter<\/jats:italic> spp., including the <jats:italic>ACBC<\/jats:italic> isolate recovered in one of the 2018 contamination events. Limits of Detection for various <jats:italic>Acinetobacter<\/jats:italic> strains were determined in dilution studies. Detection of <jats:italic>Acinetobacter<\/jats:italic> growing in platelets after an initial low inoculum was evaluated. Use of the updated test as a secondary test after pathogen reduction was also evaluated by testing at 12\u2010h intervals PR platelet units inoculated with low levels of the 3 species reported in the fatal PR platelet: <jats:italic>ACBC<\/jats:italic>, <jats:italic>Staphylococcus saprophyticus<\/jats:italic>, and <jats:italic>Leclercia adecarboxylata<\/jats:italic>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The test detected several <jats:italic>Acinetobacter<\/jats:italic> strains at the clinically relevant CFU\/ml levels associated with septic transfusions and successfully detected <jats:italic>Acinetobacter<\/jats:italic> growing in various non\u2010PR platelet types after an initial low inoculum. In PR platelets, the test yielded a positive result with the 3 implicated bacteria in 48\u2009h or less after inoculation, or 48\u201372\u2009h earlier than the reported time of transfusion of contaminated PR platelets.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>PGD<jats:italic>prime<\/jats:italic> was improved to detect <jats:italic>Acinetobacter<\/jats:italic> and has shown utility to interdict contaminated PR platelets.<\/jats:p><\/jats:sec>","DOI":"10.1111\/trf.16514","type":"journal-article","created":{"date-parts":[[2021,5,27]],"date-time":"2021-05-27T13:01:48Z","timestamp":1622120508000},"page":"2710-2717","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Evaluation of an improved rapid bacterial assay with untreated and pathogen\u2010reduced platelets: Detection of <i>Acinetobacter<\/i> strains"],"prefix":"10.1111","volume":"61","author":[{"given":"David","family":"LaVerda","sequence":"first","affiliation":[{"name":"Research and Development Department Verax Biomedical Incorporated  Marlborough Massachusetts USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lisa","family":"Shinefeld","sequence":"additional","affiliation":[{"name":"Research and Development Department Verax Biomedical Incorporated  Marlborough Massachusetts USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nancy","family":"Best","sequence":"additional","affiliation":[{"name":"Research and Development Department Verax Biomedical Incorporated  Marlborough Massachusetts USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Johny","family":"Lisitu","sequence":"additional","affiliation":[{"name":"Research and Development Department Verax Biomedical Incorporated  Marlborough Massachusetts 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