{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,22]],"date-time":"2026-04-22T05:19:53Z","timestamp":1776835193840,"version":"3.51.2"},"reference-count":16,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2021,8,9]],"date-time":"2021-08-09T00:00:00Z","timestamp":1628467200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Transfusion"],"published-print":{"date-parts":[[2021,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>In this study, we identified a novel glycophorin variant (GP.MOT) in a Mi<jats:sup>a<\/jats:sup>\u2010positive Japanese blood donor. The proband with this glycophorin variant was discovered by antigen screening of samples from 475,493 Japanese blood donors using monoclonal anti\u2010Mi<jats:sup>a<\/jats:sup>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Study design and methods<\/jats:title><jats:p>Standard serological techniques and flow cytometry were performed. GP.MOT RBCs were examined by immunoblotting using anti\u2010GPA, anti\u2010MUT or anti\u2010Mur. Genome DNA was extracted from whole blood, and the <jats:italic>GYPA<\/jats:italic>\/<jats:italic>GYPB<\/jats:italic> was analyzed by polymerase chain reactions and Sanger sequencing.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The MNS blood group of the proband was M\u2009+\u2009N\u2009+\u2009<jats:sup>w<\/jats:sup>S\u2010s\u2009+\u2009with the presence of other low\u2010frequency antigens including Mi<jats:sup>a<\/jats:sup>, Mur, MUT, and KIPP. A 43\u2010kDa molecule, which is almost equivalent in size to glycophorin A (GPA), was identified by immunoblotting using monoclonal anti\u2010MUT and anti\u2010Mur. Sanger sequencing clearly indicated that the proband had two different <jats:italic>GYPA*M<\/jats:italic> alleles at SNP rs62334651 (<jats:italic>GYPA*M232\u2009+\u200955A<\/jats:italic> and <jats:italic>GYPA*M232\u2009+ 55G<\/jats:italic>), as well as a <jats:italic>GYP<\/jats:italic>(<jats:italic>B\u2010A<\/jats:italic>) hybrid allele (<jats:italic>GYP*MOT<\/jats:italic>) with breakpoints located on pseudoexon 3 of <jats:italic>GYPB<\/jats:italic> from c.210 to c.219.<\/jats:p><\/jats:sec><jats:sec><jats:title>Discussion<\/jats:title><jats:p>We identified a hybrid glycophorin GP.MOT with the deduced unique amino acid sequence GPB (20\u201345)\u2010GP<jats:italic>\u03a8<\/jats:italic>B (46\u201370)\u2010GPA (71\u2013149), which has not been previously reported.<\/jats:p><\/jats:sec>","DOI":"10.1111\/trf.16535","type":"journal-article","created":{"date-parts":[[2021,8,9]],"date-time":"2021-08-09T12:30:38Z","timestamp":1628512238000},"page":"2825-2829","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["<scp>GP<\/scp>.<scp>MOT<\/scp>: A novel glycophorin variant identified in a Japanese blood donor"],"prefix":"10.1111","volume":"61","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2785-3375","authenticated-orcid":false,"given":"Akira","family":"Oda","sequence":"first","affiliation":[{"name":"Laboratory Japanese Red Cross Kanto\u2010Koshinetsu Block Blood Center  Tokyo Japan"},{"name":"Laboratory Japanese Red Cross Kinki Block Blood Center  Osaka Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yumi","family":"Suzuki","sequence":"additional","affiliation":[{"name":"Laboratory Japanese Red Cross Kanto\u2010Koshinetsu Block Blood Center  Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kazumi","family":"Isa","sequence":"additional","affiliation":[{"name":"Blood Group Research Unit Japanese Red Cross Central Blood Institute  Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7675-7227","authenticated-orcid":false,"given":"Kenichi","family":"Ogasawara","sequence":"additional","affiliation":[{"name":"Blood Group Research Unit Japanese Red Cross Central Blood Institute  Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ryuichi","family":"Yabe","sequence":"additional","affiliation":[{"name":"Laboratory Japanese Red Cross Kanto\u2010Koshinetsu Block Blood Center  Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takafumi","family":"Kimura","sequence":"additional","affiliation":[{"name":"Laboratory Japanese Red Cross Kinki Block Blood Center  Osaka Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Makoto","family":"Uchikawa","sequence":"additional","affiliation":[{"name":"Laboratory Japanese Red Cross Kanto\u2010Koshinetsu Block Blood Center  Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nelson Hirokazu","family":"Tsuno","sequence":"additional","affiliation":[{"name":"Laboratory Japanese Red Cross Kanto\u2010Koshinetsu Block Blood Center  Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2021,8,9]]},"reference":[{"key":"e_1_2_7_2_1","unstructured":"The International Society of Blood Transfusion: Red Cell Immunogenetics and Blood Terminology Working 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