{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T11:19:13Z","timestamp":1788347953097,"version":"build-2803163510"},"reference-count":19,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2021,6,28]],"date-time":"2021-06-28T00:00:00Z","timestamp":1624838400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Transfusion"],"published-print":{"date-parts":[[2021,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>In the setting of suspected septic transfusion reactions, bacterial culture of both the transfused patient and the residual blood component is recommended. Primary bacterial contamination can occur at the time of component collection. Clinically insignificant \u201csecondary contamination\u201d can occur during post\u2010transfusion component discard, retrieval for culture, or manipulation of the bag at the time of culture sampling.<\/jats:p><\/jats:sec><jats:sec><jats:title>Study design and methods<\/jats:title><jats:p>This retrospective, multi\u2010center study analyzes positive residual component culture results and companion patient blood cultures from 15 hospitals, 1 blood center, and all cultured transfusion reactions within the province of Quebec, Canada, over a 5\u2010year period. Imputability was assigned as \u201cdefinite\u201d (concordant growth), \u201cpossible\u201d (discordant growth or lack of growth in patient culture), or \u201cunable to assess\u201d (patient not cultured).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>There were 373 positive component cultures from 360 unique transfusion reactions, with 276 (76.7%) companion patient blood cultures performed, of which 10 (2.8%) yielded the pathogen detected in the positive component. Of these 10 definite pathogens, 7 (2 <jats:italic>Staphylococcus aureus<\/jats:italic>, 3 other staphylococci, and 1 <jats:italic>Streptococcus pyogenes<\/jats:italic> and 1 <jats:italic>Bacillus<\/jats:italic> sp.) were associated with platelet and 3 (<jats:italic>Aeromonas veronii<\/jats:italic>, <jats:italic>Staphylococcus epidermidis<\/jats:italic>, and <jats:italic>Enterococcus faecalis<\/jats:italic>) with RBC transfusions. RBC and plasma components comprised 70% of positive component cultures.<\/jats:p><\/jats:sec><jats:sec><jats:title>Discussion<\/jats:title><jats:p>The process of performing residual component culture is vulnerable to secondary contamination. The significance of microorganisms recovered from component culture cannot be interpreted in isolation. In the context of low prevalence of primary contamination of blood components, the positive predictive value of a positive component culture result is very low.<\/jats:p><\/jats:sec>","DOI":"10.1111\/trf.16545","type":"journal-article","created":{"date-parts":[[2021,6,28]],"date-time":"2021-06-28T14:57:33Z","timestamp":1624892253000},"page":"2414-2420","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Limitations of current practices in detection of bacterially contaminated blood products associated with suspected septic transfusion reactions"],"prefix":"10.1111","volume":"61","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7974-9767","authenticated-orcid":false,"given":"Isabella W.","family":"Martin","sequence":"first","affiliation":[{"name":"Department of Pathology and Laboratory Medicine Dartmouth\u2010Hitchcock Medical Center  Lebanon New Hampshire USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9847-0470","authenticated-orcid":false,"given":"Claudia S.","family":"Cohn","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine and Pathology University of Minnesota  Minneapolis Minnesota USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meghan","family":"Delaney","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine Children's National Hospital  Washington District of Columbia USA"},{"name":"Department of Laboratory Medicine University of Washington, Seattle Children's Hospital  Seattle Washington USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6731-9516","authenticated-orcid":false,"given":"Magali J.","family":"Fontaine","sequence":"additional","affiliation":[{"name":"Department of Pathology University of Maryland School of Medicine  Baltimore Maryland USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7107-2595","authenticated-orcid":false,"given":"Andrew W.","family":"Shih","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine University of British Columbia  Vancouver British Columbia Canada"},{"name":"Vancouver Coastal Health Authority  Vancouver British Columbia Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8601-5438","authenticated-orcid":false,"given":"Nancy M.","family":"Dunbar","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine Dartmouth\u2010Hitchcock Medical Center  Lebanon New Hampshire USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"name":"the SCARED Study Investigators on behalf of the Biomedical Excellence for Safer Transfusion (BEST) Collaborative","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,6,28]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.clinmicnews.2019.08.002"},{"key":"e_1_2_8_3_1","unstructured":"US Food and Drug Administration.Bacterial risk control strategies for blood collection establishments and transfusion services to enhance the safety and availability of platelets for transfusion: guidance for industry.2019.https:\/\/www.fda.gov\/regulatory-information\/search-fda-guidance-documents\/bacterial-risk-control-strategies-blood-collection-establishments-and-transfusion-services-enhance"},{"key":"e_1_2_8_4_1","unstructured":"U.S. Centers for Disease Control and Prevention.The National Healthcare Safety Network (NHSN) manual: biovigilance component v2.5 2021.http:\/\/www.cdc.gov\/nhsn\/PDFs\/Biovigilance\/BV\u2010HV\u2010protocol\u2010current.pdf. 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Guideline for investigation of suspected transfusion transmitted bacterial contamination","volume":"34","author":"Transfusion Transmitted Injuries Section","year":"2008","journal-title":"Can Commun Dis Rep Releve Mal Transm Au Can"},{"key":"e_1_2_8_12_1","volume-title":"Clinical microbiology procedures manual","author":"Ford B","year":"2016"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1423-0410.2008.01122.x"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1155\/2019\/3136493"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1086\/529143"},{"key":"e_1_2_8_16_1","unstructured":"US Food and Drug Administration.Fatalities reported to FDA following blood collection and transfusion: annual summary for FY2018."},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.15644"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.hoc.2019.05.002"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00009-19"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajic.2018.12.020"}],"container-title":["Transfusion"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/trf.16545","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/trf.16545","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/trf.16545","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,28]],"date-time":"2023-08-28T05:49:50Z","timestamp":1693201790000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/trf.16545"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,6,28]]},"references-count":19,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2021,8]]}},"alternative-id":["10.1111\/trf.16545"],"URL":"https:\/\/doi.org\/10.1111\/trf.16545","archive":["Portico"],"relation":{},"ISSN":["0041-1132","1537-2995"],"issn-type":[{"value":"0041-1132","type":"print"},{"value":"1537-2995","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,6,28]]},"assertion":[{"value":"2021-02-22","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-06-02","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-06-28","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}