{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,7]],"date-time":"2025-11-07T13:36:57Z","timestamp":1762522617914,"version":"3.40.5"},"reference-count":18,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2021,8,17]],"date-time":"2021-08-17T00:00:00Z","timestamp":1629158400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Transfusion"],"published-print":{"date-parts":[[2021,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Convalescent plasma (CP) therapy for coronavirus disease (COVID\u201019) provides virus\u2010neutralizing antibodies that may ameliorate the outcome of severe acute respiratory syndrome coronavirus 2 (SARS\u2010CoV\u20102) infections. The effectiveness of CP likely depends on its antiviral neutralizing potency and is determined using in vitro neutralizing antibody assays.<\/jats:p><\/jats:sec><jats:sec><jats:title>Study design and methods<\/jats:title><jats:p>We evaluated abilities of three immunoassays for anti\u2010spike antibodies (EUROimmun, Ortho, Roche), a pseudotype\u2010based neutralization assay, and two assays that quantify ACE2 binding of spike protein (GenScript and hemagglutination test [HAT]\u2010based assay) to predict neutralizing antibody titers in 113 CP donations. Assay outputs were analyzed through linear regression and calculation of sensitivities and specificities by receiver operator characteristic (ROC) analysis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Median values of plasma samples containing neutralizing antibodies produced conversion factors for assay unitage of \u00d76.5 (pseudotype), \u00d719 (GenScript), \u00d73.4 (HAT assay), \u00d70.08 (EUROimmun), \u00d71.64 (Roche), and \u00d70.10 (Ortho). All selected assays were sufficient in identifying the high titer donations based on ROC analysis; area over curve ranged from 91.7% for HAT and GenScript assay to 95.6% for pseudotype assay. However, their ability to predict the actual neutralizing antibody levels varied substantially as shown by linear regression correlation values (from 0.27 for Ortho to 0.61 for pseudotype assay).<\/jats:p><\/jats:sec><jats:sec><jats:title>Discussion<\/jats:title><jats:p>Overall, the study data demonstrate that all selected assays were effective in identifying donations with high neutralizing antibody levels and are potentially suitable as surrogate assays for donation selection for CP therapy.<\/jats:p><\/jats:sec>","DOI":"10.1111\/trf.16600","type":"journal-article","created":{"date-parts":[[2021,8,3]],"date-time":"2021-08-03T10:09:59Z","timestamp":1627985399000},"page":"2837-2843","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":29,"title":["Comparability of six different immunoassays measuring <scp>SARS\u2010CoV<\/scp>\u20102 antibodies with neutralizing antibody levels in convalescent plasma: From utility to prediction"],"prefix":"10.1111","volume":"61","author":[{"given":"Abigail","family":"Lamikanra","sequence":"first","affiliation":[{"name":"Clinical, Research and Development Departments NHS Blood and Transplant  Oxford UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dung","family":"Nguyen","sequence":"additional","affiliation":[{"name":"Nuffield Department of Medicine, Peter Medawar Building for Pathogen Research University of Oxford  Oxford UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7964-4700","authenticated-orcid":false,"given":"Peter","family":"Simmonds","sequence":"additional","affiliation":[{"name":"Nuffield Department of Medicine, Peter Medawar Building for Pathogen Research University of Oxford  Oxford UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sarah","family":"Williams","sequence":"additional","affiliation":[{"name":"Nuffield Department of Medicine, Peter Medawar Building for Pathogen Research University of Oxford  Oxford UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Emma M.","family":"Bentley","sequence":"additional","affiliation":[{"name":"National Institute for Biological Standards and Control (NIBSC)  South Mimms UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cathy","family":"Rowe","sequence":"additional","affiliation":[{"name":"Rare and Imported Pathogens Laboratory Public Health England  Porton Down UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ashley David","family":"Otter","sequence":"additional","affiliation":[{"name":"Rare and Imported Pathogens Laboratory Public Health England  Porton Down UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tim","family":"Brooks","sequence":"additional","affiliation":[{"name":"Rare and Imported Pathogens Laboratory Public Health England  Porton Down UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kimberly","family":"Gilmour","sequence":"additional","affiliation":[{"name":"Laboratory Services and NIHR BRC Departments Great Ormond Street Hospital  London UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Annabelle","family":"Mai","sequence":"additional","affiliation":[{"name":"Laboratory Services and NIHR BRC Departments Great Ormond Street Hospital  London UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jusvinder","family":"Dadhra","sequence":"additional","affiliation":[{"name":"Laboratory Services and NIHR BRC Departments Great Ormond Street Hospital  London UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mabel","family":"Csatari","sequence":"additional","affiliation":[{"name":"Laboratory Services and NIHR BRC Departments Great Ormond Street Hospital  London UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sheba","family":"Ziyenge","sequence":"additional","affiliation":[{"name":"Nuffield Department of Surgical Sciences and BRC Surgical Theme University of Oxford  Oxford UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marta","family":"Oliveira","sequence":"additional","affiliation":[{"name":"Nuffield Department of Surgical Sciences and BRC Surgical Theme University of Oxford  Oxford UK"},{"name":"Research Laboratory Department NHS Blood and Transplant  Oxford UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rutger","family":"Ploeg","sequence":"additional","affiliation":[{"name":"Nuffield Department of Surgical Sciences and BRC Surgical Theme University of Oxford  Oxford UK"},{"name":"Research Laboratory Department NHS Blood and Transplant  Oxford UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pat","family":"Tsang","sequence":"additional","affiliation":[{"name":"Clinical, Research and Development Departments NHS Blood and Transplant  Oxford UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria","family":"Zambon","sequence":"additional","affiliation":[{"name":"Virology Reference Department National Infection Service, Public Health England  London UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robin","family":"Gopal","sequence":"additional","affiliation":[{"name":"High Containment Microbiology Department, National Infection Service Public Health England  London UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Julie Huiyuan","family":"Xiao","sequence":"additional","affiliation":[{"name":"MRC Human Immunology Unit, MRC Weatherall Institute, Radcliffe Department of Medicine John Radcliffe Hospital  Oxford UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alain","family":"Townsend","sequence":"additional","affiliation":[{"name":"MRC Human Immunology Unit, MRC Weatherall Institute, Radcliffe Department of Medicine John Radcliffe Hospital  Oxford UK"},{"name":"Chinese Academy of Medical Science Oxford Institute, Nuffield Department of Medicine University of Oxford  Oxford UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David","family":"Roberts","sequence":"additional","affiliation":[{"name":"Clinical, Research and Development Departments NHS Blood and Transplant  Oxford UK"},{"name":"Radcliffe Department of Medicine and BRC Haematology Theme University of Oxford  Oxford UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9154-4190","authenticated-orcid":false,"given":"Heli","family":"Harvala","sequence":"additional","affiliation":[{"name":"Microbiology Services NHS Blood and Transplant  London UK"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2021,8,17]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-021-03361-1"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-021-03412-7"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.xcrm.2020.100164"},{"key":"e_1_2_7_5_1","first-page":"2021.02.05.2125","article-title":"Association of Convalescent Plasma Therapy With Survival in Patients With Hematologic Cancers and COVID\u201019","author":"Thompson MA","year":"2021","journal-title":"JAMA 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Administration. Letter of Authorization Reissuance of Convalescent Plasma EUA 2021:February 4."},{"issue":"397","key":"e_1_2_7_17_1","first-page":"2049","article-title":"Convalescent plasma in patients admitted to hospital with COVID\u201019 (RECOVERY): a randomised, controlled, open\u2010label, platform trial","volume":"29","author":"Horby PW","year":"2021","journal-title":"Lancet"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(21)00432-3"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.diagmicrobio.2020.115294"}],"container-title":["Transfusion"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/trf.16600","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/trf.16600","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/trf.16600","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,29]],"date-time":"2023-08-29T20:48:58Z","timestamp":1693342138000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/trf.16600"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,8,17]]},"references-count":18,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2021,10]]}},"alternative-id":["10.1111\/trf.16600"],"URL":"https:\/\/doi.org\/10.1111\/trf.16600","archive":["Portico"],"relation":{},"ISSN":["0041-1132","1537-2995"],"issn-type":[{"type":"print","value":"0041-1132"},{"type":"electronic","value":"1537-2995"}],"subject":[],"published":{"date-parts":[[2021,8,17]]},"assertion":[{"value":"2021-05-02","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-06-22","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-08-17","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}