{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,27]],"date-time":"2026-06-27T19:32:23Z","timestamp":1782588743559,"version":"3.54.5"},"reference-count":38,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2022,4,6]],"date-time":"2022-04-06T00:00:00Z","timestamp":1649203200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100012399","name":"Biomedical Advanced Research and Development Authority","doi-asserted-by":"publisher","award":["HHSO100201600009C"],"award-info":[{"award-number":["HHSO100201600009C"]}],"id":[{"id":"10.13039\/100012399","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100016318","name":"Cerus Corporation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100016318","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Transfusion"],"published-print":{"date-parts":[[2022,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p><jats:italic>Plasmodium falciparum<\/jats:italic> is the parasite responsible for most malaria cases globally. The risk of transfusion\u2010transmitted malaria (TTM) is mitigated by donor deferrals and blood screening strategies, which adversely impact blood availability. Previous studies showed robust inactivation of <jats:italic>P. falciparum<\/jats:italic> using nucleic acid\u2010targeting pathogen reduction technologies (PRT) for the treatment of plasma and platelet components or whole blood (WB). The efficacy of the amustaline\u2013glutathione (GSH) PRT to inactivate <jats:italic>P. falciparum<\/jats:italic> is here evaluated in red blood cells (RBC), as well the impact of PRT on parasite loads, stages, and strains.<\/jats:p><\/jats:sec><jats:sec><jats:title>Study Design and Methods<\/jats:title><jats:p>RBC units resuspended in AS\u20101 or AS\u20105 additive solutions were spiked with ring stage\u2010infected RBC and treated with the amustaline\u2013GSH PRT. Parasite loads and viability were measured in samples at the time of contamination, and after treatment, using serial 10\u2010fold dilutions of the samples in RBC cultures maintained for up to 4\u2009weeks.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p><jats:italic>P. falciparum<\/jats:italic> viability assays allow for the detection of very low levels of parasite. Initial parasite titer was &gt;5.2 log<jats:sub>10<\/jats:sub>\/ml in AS\u20101\/5 RBC. No infectious parasites were detected in amustaline\u2013GSH\u2010treated samples after 4\u2009weeks of culture. Amustaline\u2013GSH inactivated high parasite loads regardless of parasite stages and strains. Amustaline readily penetrates the parasite, irreversibly blocks development, and leads to parasite death and expulsion from RBC.<\/jats:p><\/jats:sec><jats:sec><jats:title>Discussion<\/jats:title><jats:p>Amustaline\u2013GSH PRT demonstrated robust efficacy to inactivate malaria parasites in RBC concentrates. This study completes the portfolio of studies demonstrating the efficacy of nucleic acid\u2010targeting PRTs to mitigate TTM risks as previously reported for platelet concentrates, plasma, and WB.<\/jats:p><\/jats:sec>","DOI":"10.1111\/trf.16867","type":"journal-article","created":{"date-parts":[[2022,4,6]],"date-time":"2022-04-06T10:31:17Z","timestamp":1649241077000},"page":"1073-1083","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Robust inactivation of <i>Plasmodium falciparum<\/i> in red blood cell concentrates using amustaline and glutathione pathogen reduction"],"prefix":"10.1111","volume":"62","author":[{"given":"Ciss\u00e9","family":"Sow","sequence":"first","affiliation":[{"name":"UMR7245 MCAM, Mus\u00e9um National d'Histoire Naturelle, Team PPL, CNRS  Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Am\u00e9lie","family":"Bouissou","sequence":"additional","affiliation":[{"name":"UMR7245 MCAM, Mus\u00e9um National d'Histoire Naturelle, Team PPL, CNRS  Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yvette A.","family":"Girard","sequence":"additional","affiliation":[{"name":"Cerus Corporation  Concord California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gurvani B.","family":"Singh","sequence":"additional","affiliation":[{"name":"Cerus Corporation  Concord California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lotfi","family":"Bounaadja","sequence":"additional","affiliation":[{"name":"UMR7245 MCAM, Mus\u00e9um National d'Histoire Naturelle, Team PPL, CNRS  Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean\u2010Marc","family":"Payrat","sequence":"additional","affiliation":[{"name":"Cerus Europe BV  Amersfoort Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Delphine","family":"Haas","sequence":"additional","affiliation":[{"name":"EFS Grand Est  Strasbourg France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Herv\u00e9","family":"Isola","sequence":"additional","affiliation":[{"name":"EFS Grand Est  Strasbourg France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4213-5013","authenticated-orcid":false,"given":"Marion C.","family":"Lanteri","sequence":"additional","affiliation":[{"name":"Cerus Corporation  Concord California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter","family":"Bringmann","sequence":"additional","affiliation":[{"name":"Cerus Corporation  Concord California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philippe","family":"Grellier","sequence":"additional","affiliation":[{"name":"UMR7245 MCAM, Mus\u00e9um National d'Histoire Naturelle, Team PPL, CNRS  Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,4,6]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.30875\/60123dd4-en"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.15585\/mmwr.ss6805a1"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2012.03673.x"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1186\/1475-2875-12-439"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/tid.12938"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1503\/cmaj.090171"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12936-018-2181-0"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.3201\/eid0808.020025"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1423-0410.2006.00733.x"},{"key":"e_1_2_7_11_1","first-page":"551","volume-title":"Malariology","author":"Boyd M","year":"1949"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/vox.12142"},{"issue":"2","key":"e_1_2_7_13_1","first-page":"94","article-title":"Malaria blood safety policy in five non\u2010endemic countries: a retrospective comparison through the lens of the ABO risk\u2010based decision\u2010making framework","volume":"17","author":"O'Brien SF","year":"2019","journal-title":"Blood Transfus"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2008.01825.x"},{"key":"e_1_2_7_15_1","unstructured":"WHO.Screening Donated Blood for Transfusion\u2010 Transmissible Infections Recommendations [Internet]. 2010. 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