{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T18:44:32Z","timestamp":1761677072644,"version":"3.40.5"},"reference-count":6,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2022,4,11]],"date-time":"2022-04-11T00:00:00Z","timestamp":1649635200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Transfusion"],"published-print":{"date-parts":[[2022,5]]},"abstract":"Abstract<\/jats:title>Background<\/jats:title>A 2\u2010year\u2010old, 10.8\u00a0kg male pediatric patient with X\u2010linked chronic granulomatous disease (CGD) with McLeod syndrome (MLS) was scheduled for a hematopoietic stem cell transplant (HSCT). Identification of allogenic red blood cells (RBC) for post\u2010transplant support was unsuccessful prompting the development of a customized method to collect and freeze rare autologous pediatric cells.<\/jats:p><\/jats:sec>Study Design and Methods<\/jats:title>A protocol was developed for the collection of small volume pediatric whole blood (WB) via peripheral venipuncture with collection into 10\u00a0ml syringes containing anticoagulants. Additionally, a closed system RBC glycerolization and deglycerolization instrument was adapted to process small volume, non\u2010leukoreduced WB. Both collection and WB processes were validated. In total 4 approximately 100\u2009ml autologous units were collected and frozen. Two units were thawed, deglycerolized, and used for clinical transfusion support. To appreciate processing impacts on RBC rigidity, ektacytometry was performed on pre\u2010processed and post\u2010deglycerolization samples.<\/jats:p><\/jats:sec>Results<\/jats:title>Free hemoglobin (HGB) of validation units after thawing\/deglycerolization was <150\u2009mg\/dL with an average red cell recovery of 85%. These units also showed little difference between pre\u2010and post\u2010processing Lorrca deformability curves or membrane rigidity. Two pediatric units were thawed and deglycerolized for transfusion. Free HGB was 70\u2009mg\/dL and 50\u2009mg\/dL post\u2010thaw, and these RBCs had a slight decrease in deformability and increased membrane rigidity.<\/jats:p><\/jats:sec>Discussion<\/jats:title>Customized WB collection, glycerolization, freezing, and deglycerolization processes were developed to successfully support a pediatric patient with CGD and MLS after autologous HSCT. Both pediatric units showed increased membrane rigidity post\u2010deglycerolization which may be a consequence of the CGD and MLS genetic background.<\/jats:p><\/jats:sec>","DOI":"10.1111\/trf.16878","type":"journal-article","created":{"date-parts":[[2022,4,11]],"date-time":"2022-04-11T12:13:14Z","timestamp":1649679194000},"page":"954-960","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Cryopreservation of rare pediatric red blood cells for support following bone marrow transplant"],"prefix":"10.1111","volume":"62","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1102-6279","authenticated-orcid":false,"given":"Kathleen","family":"Kelly","sequence":"first","affiliation":[{"name":"Vitalant Research Institute Denver Colorado USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Louise","family":"Helander","sequence":"additional","affiliation":[{"name":"ClinImmune Labs\/Department of Medicine University of Colorado Aurora Colorado USA"},{"name":"Department of Pathology and Laboratory Medicine Children's Hospital Colorado Aurora Colorado USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kelsey","family":"Hazegh","sequence":"additional","affiliation":[{"name":"Vitalant Research Institute Denver Colorado USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Crystal","family":"Stanley","sequence":"additional","affiliation":[{"name":"Vitalant Denver Colorado USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Raymond","family":"Moss","sequence":"additional","affiliation":[{"name":"Vitalant Research Institute Denver Colorado USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Samantha","family":"Mack","sequence":"additional","affiliation":[{"name":"Vitalant Denver Colorado USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mary L.","family":"Sanders","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine Children's Hospital Colorado Aurora Colorado USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Janice","family":"Gurley","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine Children's Hospital Colorado Aurora Colorado USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chris","family":"McKinney","sequence":"additional","affiliation":[{"name":"Center for Cancer and Blood Disorders Children's Hospital Colorado Aurora Colorado USA"},{"name":"Medical Department of Pediatrics University of Colorado Aurora Colorado USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1715-0748","authenticated-orcid":false,"given":"Larry J.","family":"Dumont","sequence":"additional","affiliation":[{"name":"Vitalant Research Institute Denver Colorado USA"},{"name":"Department of Pathology and Laboratory Medicine University of Colorado Medical School Aurora Colorado USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kyle","family":"Annen","sequence":"additional","affiliation":[{"name":"Department of Pathology University of Colorado Aurora Colorado USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2022,4,11]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bmt.1704524"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/bmt.2008.159"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/bmt.2009.115"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.21307\/immunohematology-2021-012"},{"volume-title":"Standards for Blood Banks and Transfusion Services","year":"2020","key":"e_1_2_8_6_1"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1537-2995.1990.30891020333.x"}],"container-title":["Transfusion"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/trf.16878","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/trf.16878","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/trf.16878","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,25]],"date-time":"2023-08-25T15:04:36Z","timestamp":1692975876000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/trf.16878"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,4,11]]},"references-count":6,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2022,5]]}},"alternative-id":["10.1111\/trf.16878"],"URL":"https:\/\/doi.org\/10.1111\/trf.16878","archive":["Portico"],"relation":{},"ISSN":["0041-1132","1537-2995"],"issn-type":[{"type":"print","value":"0041-1132"},{"type":"electronic","value":"1537-2995"}],"subject":[],"published":{"date-parts":[[2022,4,11]]},"assertion":[{"value":"2022-01-12","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-03-08","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-04-11","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}