{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T01:22:44Z","timestamp":1787016164940,"version":"build-2736575974"},"reference-count":26,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2022,7,22]],"date-time":"2022-07-22T00:00:00Z","timestamp":1658448000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Transfusion"],"published-print":{"date-parts":[[2022,8]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Anti\u2010CD38 antibodies such as daratumumab (DARA) are critical therapies for multiple myeloma and other diseases. Unfortunately, anti\u2010CD38 antibodies cause panreactivity in indirect antiglobulin tests (IATs), complicating blood compatibility testing. The anti\u2010CD38 interference is most often mitigated by treating reagent red blood cells (RBCs) with dithiothreitol (DTT). However, when using the DTT method, not all RBC antibody specificities can be detected (e.g., anti\u2010K), and the DTT method is impractical for some transfusion services. We evaluated the ability of a new anti\u2010idiotype antibody to neutralize DARA in vitro and eliminate the anti\u2010CD38 interference.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Study Design and Methods<\/jats:title>\n                    <jats:p>A recombinant monoclonal rabbit anti\u2010DARA idiotype antibody (\u201canti\u2010DARA\u201d) was generated. The ratio of anti\u2010DARA required to neutralize DARA in spiked samples was evaluated in IATs performed in gel. IATs performed in tube were used to demonstrate that anti\u2010DARA allows alloantibody detection in the presence of DARA. Plasma samples from 29 patients receiving DARA were treated with a fixed quantity of anti\u2010DARA (120\u2009\u03bcg) before performing antibody detection tests (screens) in tube.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Anti\u2010DARA used at or above a 1:1 ratio with DARA eliminated the DARA interference with IATs. Anti\u2010DARA allowed detection of both alloanti\u2010E and alloanti\u2010K in the presence of DARA. In 27\/29 (93.1%) clinical samples, 120\u2009\u03bcg anti\u2010DARA was sufficient to neutralize the DARA in 100\u2009\u03bcl patient plasma.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Discussion<\/jats:title>\n                    <jats:p>An anti\u2010DARA:DARA ratio as low as 1:1 is sufficient to neutralize DARA in solution. A fixed amount of anti\u2010DARA eliminates the anti\u2010CD38 interference in most patient samples.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/trf.17006","type":"journal-article","created":{"date-parts":[[2022,7,22]],"date-time":"2022-07-22T07:04:29Z","timestamp":1658473469000},"page":"1511-1518","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Efficient neutralization of daratumumab in pretransfusion samples using a novel recombinant monoclonal anti\u2010idiotype antibody"],"prefix":"10.1111","volume":"62","author":[{"given":"Fleur","family":"Aung","sequence":"first","affiliation":[{"name":"Department of Laboratory Medicine MD Anderson Cancer Center  Houston Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeff","family":"Spencer","sequence":"additional","affiliation":[{"name":"Helena Laboratories  Beaumont Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Potter","sequence":"additional","affiliation":[{"name":"Fortis Life Sciences  Montgomery Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thuy\u2010Dung","family":"Pham","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine MD Anderson Cancer Center  Houston Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naheed","family":"Farooqui","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine MD Anderson Cancer Center  Houston Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kathryn R.","family":"Platt","sequence":"additional","affiliation":[{"name":"Department of Pathology Brigham and Women's Hospital  Boston Massachusetts USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raja","family":"Zayat","sequence":"additional","affiliation":[{"name":"Department of 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