{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,27]],"date-time":"2026-02-27T02:55:21Z","timestamp":1772160921794,"version":"3.50.1"},"reference-count":14,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2022,7,14]],"date-time":"2022-07-14T00:00:00Z","timestamp":1657756800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Transfusion"],"published-print":{"date-parts":[[2022,9]]},"abstract":"Abstract<\/jats:title>\n \n Background<\/jats:title>\n A direct antiglobulin test (DAT) checks for antibody or complement on the surface of RBCs and is often done following a transfusion reaction. While passive anti\u2010A and anti\u2010B antibodies are known to cause positive DATs, the extent this occurs following transfusion is unknown.<\/jats:p>\n <\/jats:sec>\n \n Study Design and Methods<\/jats:title>\n DAT results, ABO type, eluate information, and blood product information were recorded on 1097 transfusion reactions at a large academic hospital over 8\u2009years. The effect of patient blood type, product type, and plasma compatibility of blood product transfused on DAT results were determined. Statistical significance was determined using Chi\u2010squared testing.<\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n Patient ABO blood type was a strong predictor of a positive DAT, with type O patients having 6.7% positive rate and non\u2010O patients having a positive rate of 20.6% (\n p<\/jats:italic>\n \u00a0<\u2009.0001). Plasma compatibility of the product was a strong predictor of a positive DAT, with plasma compatible transfusions having a 9.4% positive rate while plasma incompatible transfusions were positive 44% of the time (\n p<\/jats:italic>\n \u00a0<\u2009.0001). Elution studies found that anti\u2010A\/B antibodies were the most common antibody identified. Platelets were more likely to be associated with a positive DAT when compared with RBC transfusions (\n p<\/jats:italic>\n \u00a0<\u2009.05).\n <\/jats:p>\n <\/jats:sec>\n \n Conclusions<\/jats:title>\n These results demonstrate the patient ABO type and plasma incompatibility are strong predictors of positive DAT results following a transfusion reaction. Anti\u2010A and anti\u2010B antibodies are estimated to account for about 50% of positive DATs in this study.<\/jats:p>\n <\/jats:sec>","DOI":"10.1111\/trf.17019","type":"journal-article","created":{"date-parts":[[2022,7,15]],"date-time":"2022-07-15T03:13:05Z","timestamp":1657854785000},"page":"1715-1719","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Patient\n ABO<\/scp>\n blood type is a major predictor of a positive\n DAT<\/scp>\n following a transfusion reaction"],"prefix":"10.1111","volume":"62","author":[{"given":"Eric","family":"Schnieders","sequence":"first","affiliation":[{"name":"DeGowin Blood Center, Department of Pathology University of Iowa Hospitals & Clinics Iowa City Iowa USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Judith","family":"Leon","sequence":"additional","affiliation":[{"name":"DeGowin Blood Center, Department of Pathology University of Iowa Hospitals & Clinics Iowa City Iowa USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"James M.","family":"Chapman","sequence":"additional","affiliation":[{"name":"DeGowin Blood Center, Department of Pathology University of Iowa Hospitals & Clinics Iowa City Iowa USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3964-5466","authenticated-orcid":false,"given":"C. Michael","family":"Knudson","sequence":"additional","affiliation":[{"name":"DeGowin Blood Center, Department of Pathology University of Iowa Hospitals & Clinics Iowa City Iowa USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2022,7,14]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/ajh.23218"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.5858\/arpa.2015-0444-RS"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra1802338"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/ajcp\/109.6.769"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.transci.2013.09.017"},{"key":"e_1_2_7_7_1","first-page":"35","article-title":"Coombs positive\u201d \u201cnormal\u201d people","volume":"23","author":"Weiner W","year":"1965","journal-title":"Bibl Haematol"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.13069"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.14900"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.15033"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1537-2995.1986.26386209372.x"},{"key":"e_1_2_7_12_1","first-page":"378","article-title":"Hemolytic transfusion reaction. Recent experience in a large blood bank","volume":"53","author":"Pineda AA","year":"1978","journal-title":"Mayo Clin Proc"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1537-2995.1990.30891020325.x"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.14057"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.15655"}],"container-title":["Transfusion"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/trf.17019","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/trf.17019","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/trf.17019","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,21]],"date-time":"2023-08-21T18:12:58Z","timestamp":1692641578000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/trf.17019"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,7,14]]},"references-count":14,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2022,9]]}},"alternative-id":["10.1111\/trf.17019"],"URL":"https:\/\/doi.org\/10.1111\/trf.17019","archive":["Portico"],"relation":{"has-preprint":[{"id-type":"doi","id":"10.1101\/2021.11.01.21265756","asserted-by":"object"}]},"ISSN":["0041-1132","1537-2995"],"issn-type":[{"value":"0041-1132","type":"print"},{"value":"1537-2995","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,7,14]]},"assertion":[{"value":"2021-10-29","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-05-18","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-07-14","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}