{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,19]],"date-time":"2026-10-19T21:43:05Z","timestamp":1792446185951,"version":"4.5.0"},"reference-count":20,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2022,7,19]],"date-time":"2022-07-19T00:00:00Z","timestamp":1658188800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["324392634\u2013TRR221"],"award-info":[{"award-number":["324392634\u2013TRR221"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Transfusion"],"published-print":{"date-parts":[[2022,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>To date, in\u2010depth analysis of leukapheresis products as starting material for CAR T\u2010cell manufacturing, specifically Tisagenlecleucel production, are scarce. In this study, we report on lymphapheresis data for production of Tisagenlecleucel for elderly and pretreated lymphoma patients.<\/jats:p><\/jats:sec><jats:sec><jats:title>Study Design and Methods<\/jats:title><jats:p>Spectra Optia from Terumo BCT, Lakewood, CO, was employed for apheresis using the cMNC program. Apheresis success was defined as meeting a target total nucleated cell (TNC) count of \u22652\u2009\u00d7\u200910<jats:sup>9<\/jats:sup>, a CD3\u2010positive lymphocyte count of \u22651\u2009\u00d7\u200910<jats:sup>9<\/jats:sup> and an overall viability of \u226570% in the lymphapheresis product.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Twenty\u2010three patients (age 37\u201377\u2009years) and 24 apheresis runs were evaluated. The median CD3\u2010positive lymphocyte count in peripheral blood at the beginning of apheresis was 565 cells\/\u03bcl (range: 70\u20131345 cells\/\u03bcl). Circulating lymphoma cells were detected in one patient prior to apheresis. Target criteria were met in 21 of 23 patients. The median TNC count in the apheresate was 11.2\u2009\u00d7\u200910<jats:sup>9<\/jats:sup> (range: 2.9\u2009\u00d7\u200910<jats:sup>9<\/jats:sup>\u201347.4\u2009\u00d7\u200910<jats:sup>9<\/jats:sup>). The median CD3\u2010positive lymphocyte count in the apheresate was 2.55\u2009\u00d7\u200910<jats:sup>9<\/jats:sup> (range: 0.370\u2009\u00d7\u200910<jats:sup>9<\/jats:sup>\u20136.915\u2009\u00d7\u200910<jats:sup>9<\/jats:sup>), which resulted in a median collection efficiency for CD3\u2010positive lymphocytes of 63.7% (range: 9.56%\u201393.6%). No adverse events associated with the apheresis process were observed.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Lymphapheresis with the Spectra Optia cMNC program provided a sufficient quantity of CD3\u2010positive lymphocytes for CAR T\u2010cell manufacturing for the majority of patients despite their heavy pretreatment and advanced age. Moreover, we are the first to advocate early pre\u2010emptive lymphocyte collection in DLBCL\u2010NOS patients intended to undergo treatment with Tisagenlecleucel.<\/jats:p><\/jats:sec>","DOI":"10.1111\/trf.17030","type":"journal-article","created":{"date-parts":[[2022,7,19]],"date-time":"2022-07-19T09:35:03Z","timestamp":1658223303000},"page":"1602-1611","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["Apheresis for chimeric antigen receptor T\u2010cell production in adult lymphoma patients"],"prefix":"10.1111","volume":"62","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7512-572X","authenticated-orcid":false,"given":"Dennis Christoph","family":"Harrer","sequence":"first","affiliation":[{"name":"Department of Internal Medicine III, Hematology and Oncology University Hospital Regensburg  Regensburg 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Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4115-8405","authenticated-orcid":false,"given":"Viola","family":"Haehnel","sequence":"additional","affiliation":[{"name":"Institute for Clinical Chemistry, Laboratory Medicine and Transfusion Medicine University Hospital Regensburg  Regensburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert","family":"Offner","sequence":"additional","affiliation":[{"name":"Institute for Clinical Chemistry, Laboratory Medicine and Transfusion Medicine University Hospital Regensburg  Regensburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ralph","family":"Burkhardt","sequence":"additional","affiliation":[{"name":"Institute for Clinical Chemistry, Laboratory Medicine and Transfusion Medicine University Hospital Regensburg  Regensburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wolfgang","family":"Herr","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine III, Hematology and Oncology University Hospital Regensburg  Regensburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthias","family":"Edinger","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine III, Hematology and Oncology University Hospital Regensburg  Regensburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel","family":"Wolff","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine III, Hematology and Oncology University Hospital Regensburg  Regensburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Simone","family":"Thomas","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine III, Hematology and Oncology University Hospital Regensburg  Regensburg Germany"},{"name":"Leibniz Institute for Immunotherapy Division of Genetic\u2010Immunotherapy  Regensburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andreas","family":"Brosig","sequence":"additional","affiliation":[{"name":"Institute for Clinical Chemistry, Laboratory Medicine and Transfusion Medicine University Hospital Regensburg  Regensburg 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