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Because platelets are replaced faster than red blood cells, as many as 24 donations can be done annually. However, some frequent apheresis platelet donors (&gt;20 donations annually) display severe plateletpheresis\u2010associated lymphopenia; in particular, CD4<jats:sup>+<\/jats:sup>T but not B cell numbers are decreased. COVID\u201019 vaccination thereby provides a model to assess whether lymphopenic platelet donors present compromised humoral immune responses.<\/jats:p><\/jats:sec><jats:sec><jats:title>Study Design and Methods<\/jats:title><jats:p>We assessed vaccine responses following 2 doses of COVID\u201019 vaccination in a cohort of 43 plateletpheresis donors with a range of pre\u2010vaccination CD4<jats:sup>+<\/jats:sup>T cell counts (76\u20131537 cells\/\u03bcl). In addition to baseline T cell measurements, antibody binding assays to full\u2010length Spike and the Receptor Binding Domain (RBD) were performed pre\u2010 and post\u2010vaccination. Furthermore, pseudo\u2010particle neutralization and antibody\u2010dependent cellular cytotoxicity assays were conducted to measure antibody functionality.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Participants were stratified into two groups: &lt;400 CD4\/\u03bcl (<jats:italic>n<\/jats:italic>\u00a0=\u00a027) and\u2009\u2265\u2009400 CD4\/\u03bcl (<jats:italic>n<\/jats:italic>\u00a0=\u00a016). Following the first dose, 79% seroconverted within the &lt;400 CD4\/\u03bcl group compared to 87% in the \u2265400 CD4\/\u03bcl group; all donors were seropositive post\u2010second dose with significant increases in antibody levels. Importantly differences in CD4<jats:sup>+<\/jats:sup> T cell levels minimally impacted neutralization, Spike recognition, and IgG Fc\u2010mediated effector functions.<\/jats:p><\/jats:sec><jats:sec><jats:title>Discussion<\/jats:title><jats:p>Overall, our results indicate that lymphopenic plateletpheresis donors do not exhibit significant immune dysfunction; they have retained the T and B cell functionality necessary for potent antibody responses after vaccination.<\/jats:p><\/jats:sec>","DOI":"10.1111\/trf.17037","type":"journal-article","created":{"date-parts":[[2022,8,3]],"date-time":"2022-08-03T05:58:36Z","timestamp":1659506316000},"page":"1779-1790","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["<scp>COVID<\/scp>\u201019 vaccine humoral response in frequent platelet donors with plateletpheresis\u2010associated lymphopenia"],"prefix":"10.1111","volume":"62","author":[{"given":"Annemarie Eare","family":"Laumaea","sequence":"first","affiliation":[{"name":"Centre de Recherche du CHUM  Montr\u00e9al Canada"},{"name":"D\u00e9partement de Microbiologie, Infectiologie et Immunologie Universit\u00e9 de Montr\u00e9al  Montr\u00e9al Qu\u00e9bec Canada"},{"name":"H\u00e9ma\u2010Qu\u00e9bec Affaires M\u00e9dicales et Innovation  Qu\u00e9bec Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1748-4198","authenticated-orcid":false,"given":"Antoine","family":"Lewin","sequence":"additional","affiliation":[{"name":"H\u00e9ma\u2010Qu\u00e9bec Affaires M\u00e9dicales et Innovation  Montr\u00e9al Qu\u00e9bec Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Debashree","family":"Chatterjee","sequence":"additional","affiliation":[{"name":"Centre de Recherche du CHUM  Montr\u00e9al Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lorie","family":"Marchitto","sequence":"additional","affiliation":[{"name":"Centre de Recherche du CHUM  Montr\u00e9al Canada"},{"name":"D\u00e9partement de Microbiologie, Infectiologie et Immunologie Universit\u00e9 de Montr\u00e9al  Montr\u00e9al Qu\u00e9bec Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shilei","family":"Ding","sequence":"additional","affiliation":[{"name":"Centre de Recherche du CHUM  Montr\u00e9al Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gabrielle","family":"Gendron\u2010Lepage","sequence":"additional","affiliation":[{"name":"Centre de Recherche du CHUM  Montr\u00e9al Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guillaume","family":"Goyette","sequence":"additional","affiliation":[{"name":"Centre de Recherche du CHUM  Montr\u00e9al Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marie\u2010\u00c8ve","family":"Allard","sequence":"additional","affiliation":[{"name":"H\u00e9ma\u2010Qu\u00e9bec Affaires M\u00e9dicales et Innovation  Qu\u00e9bec Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carl","family":"Simard","sequence":"additional","affiliation":[{"name":"H\u00e9ma\u2010Qu\u00e9bec Affaires M\u00e9dicales et Innovation  Qu\u00e9bec Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tony","family":"Tremblay","sequence":"additional","affiliation":[{"name":"H\u00e9ma\u2010Qu\u00e9bec Affaires M\u00e9dicales et Innovation  Qu\u00e9bec Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9e","family":"Perreault","sequence":"additional","affiliation":[{"name":"H\u00e9ma\u2010Qu\u00e9bec Affaires M\u00e9dicales et Innovation  Qu\u00e9bec Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ralf","family":"Duerr","sequence":"additional","affiliation":[{"name":"Department of Microbiology New York University School of Medicine  New York City New York USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4992-5288","authenticated-orcid":false,"given":"Andr\u00e9s","family":"Finzi","sequence":"additional","affiliation":[{"name":"Centre de Recherche du CHUM  Montr\u00e9al Canada"},{"name":"D\u00e9partement de Microbiologie, Infectiologie et Immunologie Universit\u00e9 de Montr\u00e9al  Montr\u00e9al Qu\u00e9bec Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9594-2532","authenticated-orcid":false,"given":"Ren\u00e9e","family":"Bazin","sequence":"additional","affiliation":[{"name":"H\u00e9ma\u2010Qu\u00e9bec Affaires M\u00e9dicales et Innovation  Qu\u00e9bec Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,8,2]]},"reference":[{"key":"e_1_2_9_2_1","unstructured":"Hema Qu\u00e9bec. 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