{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,14]],"date-time":"2025-05-14T02:05:11Z","timestamp":1747188311982,"version":"3.40.5"},"reference-count":18,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2022,9,5]],"date-time":"2022-09-05T00:00:00Z","timestamp":1662336000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Transfusion"],"published-print":{"date-parts":[[2022,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Low\u2010prevalence antigen s<jats:sup>D<\/jats:sup> (MNS23) is encoded by <jats:italic>GYPB<\/jats:italic> c.173C\u2009&gt;\u2009G. Hemolytic disease of the fetus and newborn (HDFN) due to anti\u2010s<jats:sup>D<\/jats:sup> is rare. A mother delivered a newborn whose red blood cells (RBCs) were DAT\u2010positive and was later diagnosed with HDFN. Serum from the mother was incompatible with the father's RBCs and was used to screen 184 Thai blood donors. This study aimed to investigate the cause of HDFN in a Thai family and determine the prevalence of s<jats:sup>D<\/jats:sup> in Thai blood donors.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and Methods<\/jats:title><jats:p>Three family members and four blood donors were investigated in the study. Massively Parallel Sequencing (MPS) was used for genotyping. Standard hemagglutination techniques were used in titration studies, phenotyping, and enzyme\/chemical studies. Anti\u2010s, anti\u2010Mi<jats:sup>a<\/jats:sup>, anti\u2010JENU, and anti\u2010s<jats:sup>D<\/jats:sup> reagents were used in serological investigations.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The mother was <jats:italic>GYP*Mur\/Mur<\/jats:italic>. The father and the four donors were <jats:italic>GYPB*s\/s<\/jats:italic><jats:sup><jats:italic>D<\/jats:italic><\/jats:sup> predicting S\u2009\u2212\u2009s\u2009+\u2009s<jats:sup>D<\/jats:sup>+. The baby was <jats:italic>GYP*Mur\/sD<\/jats:italic> and his RBCs were Mi<jats:sup>a<\/jats:sup>+, s\u2009+\u00a0<jats:sup>w<\/jats:sup> with anti\u2010s (P3BER) and JENU+<jats:sup>w<\/jats:sup>. RBCs from two <jats:italic>GYPB*s<\/jats:italic><jats:sup><jats:italic>D<\/jats:italic><\/jats:sup>\u2010positive blood donors reacted with anti\u2010s<jats:sup>D<\/jats:sup> (Dreyer). Proteolytic enzyme \u03b1\u2010chymotrypsin\u2010treated s<jats:sup>D<\/jats:sup>+ cells did not react with anti\u2010s<jats:sup>D<\/jats:sup> (Wat) produced by the GP.Mur\/Mur mother but reacted with the original anti\u2010s<jats:sup>D<\/jats:sup> (Dreyer).<\/jats:p><\/jats:sec><jats:sec><jats:title>Discussion<\/jats:title><jats:p>This is the first report of HDFN due to anti\u2010s<jats:sup>D<\/jats:sup> in the Asian population. The genotype frequency for <jats:italic>GYPB*s<\/jats:italic><jats:sup><jats:italic>D<\/jats:italic><\/jats:sup> in a selected Thai blood donor population is 2.2% (4\/184). Anti\u2010s<jats:sup>D<\/jats:sup> should be considered in mothers with Southeast Asian or East Asian background when antibody identification is unresolved in pregnancies affected by HDFN.<\/jats:p><\/jats:sec>","DOI":"10.1111\/trf.17086","type":"journal-article","created":{"date-parts":[[2022,9,5]],"date-time":"2022-09-05T09:48:53Z","timestamp":1662371333000},"page":"2137-2142","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Hemolytic disease of the fetus and newborn caused by <scp>anti\u2010s<sup>D<\/sup><\/scp> antibody in a <scp>GP<\/scp>.Mur\/Mur Thai mother and review of the prevalence of <scp>s<sup>D<\/sup><\/scp> in Thai blood donors"],"prefix":"10.1111","volume":"62","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8568-0604","authenticated-orcid":false,"given":"Genghis H.","family":"Lopez","sequence":"first","affiliation":[{"name":"Research and Development, Clinical Services and Research Australian Red Cross Lifeblood  Kelvin Grove Queensland Australia"},{"name":"School of Health and Behavioural Sciences University of the Sunshine Coast  Sippy Downs Queensland Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Morakot","family":"Emthip","sequence":"additional","affiliation":[{"name":"Red Cell Reference Laboratory National Blood Centre, Thai Red Cross Society  Bangkok Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ploymanee","family":"Suwanwootichai","sequence":"additional","affiliation":[{"name":"Red Cell Reference Laboratory National Blood Centre, Thai Red Cross Society  Bangkok Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3809-9662","authenticated-orcid":false,"given":"Glenda M.","family":"Millard","sequence":"additional","affiliation":[{"name":"Research and Development, Clinical Services and Research Australian Red Cross Lifeblood  Kelvin Grove Queensland 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Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pimol","family":"Chiewsilp","sequence":"additional","affiliation":[{"name":"Red Cell Reference Laboratory National Blood Centre, Thai Red Cross Society  Bangkok Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert L.","family":"Flower","sequence":"additional","affiliation":[{"name":"Research and Development, Clinical Services and Research Australian Red Cross Lifeblood  Kelvin Grove Queensland Australia"},{"name":"School of Biomedical Sciences, Faculty of Health Queensland University of Technology  Brisbane Queensland Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4124-6168","authenticated-orcid":false,"given":"Catherine A.","family":"Hyland","sequence":"additional","affiliation":[{"name":"Research and Development, Clinical Services and Research Australian Red Cross Lifeblood  Kelvin Grove Queensland Australia"},{"name":"School of Biomedical Sciences, Faculty of Health Queensland University of Technology  Brisbane Queensland Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yew\u2010Wah","family":"Liew","sequence":"additional","affiliation":[{"name":"Red Cell Reference Laboratory Clinical Services and Research, Australian Red Cross Lifeblood  Kelvin Grove Queensland Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2022,9,5]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.21307\/immunohematology-2019-240"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.21037\/aob-21-9"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.13952"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/vox.12909"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pathol.2017.12.289"},{"key":"e_1_2_8_7_1","first-page":"614","article-title":"Serology 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