{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,12]],"date-time":"2026-06-12T03:51:19Z","timestamp":1781236279160,"version":"3.54.1"},"reference-count":60,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2022,10,30]],"date-time":"2022-10-30T00:00:00Z","timestamp":1667088000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Transfusion"],"published-print":{"date-parts":[[2023,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Red cell (RBC) transfusions are beneficial for patients with sickle cell disease (SCD), but ex vivo studies suggest that inflamed plasma from patients with SCD during crises may damage these RBCs, diminishing their potential efficacy. The hypoxic storage of RBCs may improve transfusion efficacy by minimizing the storage lesion. We tested the hypotheses that (1) The donor RBCs exposed to the plasma of patients in crisis would have lower deformability and higher hemolysis than those exposed to non\u2010crisis plasma, and (2) hypoxic storage, compared to standard storage, of donor RBCs could preserve deformability and reduce hemolysis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Study Design and Methods<\/jats:title><jats:p>18 <jats:styled-content style=\"fixed-case\">SCD<\/jats:styled-content> plasma samples from patients who had severe acute\u2010phase symptoms (A\u2010plasma; <jats:italic>n<\/jats:italic>\u00a0=\u00a09) or were at a steady\u2010state (S\u00a0=\u00a0plasma; <jats:italic>n<\/jats:italic>\u00a0=\u00a09) were incubated with 16 <jats:styled-content style=\"fixed-case\">RBC<\/jats:styled-content> samples from eight units that were stored either under conventional(<jats:styled-content style=\"fixed-case\">CRBC<\/jats:styled-content>) or hypoxic(<jats:styled-content style=\"fixed-case\">HRBC<\/jats:styled-content>) conditions. Hemolysis and microcapillary deformability assays of these <jats:styled-content style=\"fixed-case\">RBCs<\/jats:styled-content> were analyzed using linear mixed\u2010effect models after each sample was incubated in patient plasma overnight at <jats:styled-content style=\"fixed-case\">37\u00b0C<\/jats:styled-content><\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Relative deformability was 0.036 higher (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.0001) in HRBC pairs compared to CRBC pairs regardless of plasma type. Mean donor RBC hemolysis was 0.33% higher after incubation with A\u2010plasma compared to S\u2010plasma either with HRBC or CRBC (<jats:italic>p<\/jats:italic>\u00a0=\u00a00.04). HRBCs incubated with steady\u2010state patient plasma demonstrated the highest deformability and lowest hemolysis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Hypoxic storage significantly influenced RBC deformability. Patient condition significantly influenced post\u2010incubation hemolysis. Together, HRBCs in steady\u2010state plasma maximized donor red cell ex vivo function and survival.<\/jats:p><\/jats:sec>","DOI":"10.1111\/trf.17163","type":"journal-article","created":{"date-parts":[[2022,10,31]],"date-time":"2022-10-31T06:47:19Z","timestamp":1667198839000},"page":"193-202","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Hypoxic storage of donor red cells preserves deformability after exposure to plasma from adults with sickle cell disease"],"prefix":"10.1111","volume":"63","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7533-6842","authenticated-orcid":false,"given":"Matthew S.","family":"Karafin","sequence":"first","affiliation":[{"name":"Department of Pathology and Laboratory Medicine University of North Carolina  Chapel Hill North Carolina USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joshua J.","family":"Field","sequence":"additional","affiliation":[{"name":"Division 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S.","family":"Shevkoplyas","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering University of Houston  Houston Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3879-5668","authenticated-orcid":false,"given":"Tatsuro","family":"Yoshida","sequence":"additional","affiliation":[{"name":"Hemanext Inc  Lexington Massachusetts 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