{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,14]],"date-time":"2025-05-14T02:05:28Z","timestamp":1747188328141,"version":"3.40.5"},"reference-count":26,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2022,11,25]],"date-time":"2022-11-25T00:00:00Z","timestamp":1669334400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Transfusion"],"published-print":{"date-parts":[[2023,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Management of platelet\u2010transfusion refractory (PR) patients due to anti\u2010HLA antibodies includes the provision of HLA\u2010matched (HLAm) platelets (PLT) or PLTs that are negative for HLA antigens corresponding to the recipient antibodies. Obtaining HLAm PLTs is predicated on accurate HLA antigen typing of the recipient and donor. Here, we present the clinical implications of a case involving loss of heterozygosity (LOH) in a patient presented for PR workup.<\/jats:p><\/jats:sec><jats:sec><jats:title>Study Design and Methods<\/jats:title><jats:p>HLA typing was performed by three methods: (1) Real\u2010time PCR; (2) Sequence\u2010specific oligonucleotide (SSO) typing test; and (3) Next\u2010Generation Sequencing (NGS). Cytogenomic SNP microarray was utilized to assess LOH.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>A 30\u2010year\u2010old female with newly diagnosed acute myelogenous leukemia was found to be PR secondary to HLA sensitization. A peripheral blood (PB) sample, containing 93% myeloid blast cells, was sent for HLA typing for the provision of HLAm PLTs. HLA typing revealed homozygosity at the <jats:italic>HLA\u2010A<\/jats:italic> locus but was heterozygous at the \u2010B and \u2010C loci. After chemotherapy, HLA typing on a new PB sample, devoid of blast cells, identified HLA\u2010A locus heterozygosity, which was subsequently confirmed by real\u2010time PCR and NGS. Cytogenomic SNP microarray analysis demonstrated LOH of the <jats:italic>HLA\u2010A<\/jats:italic> locus on chromosome 6p in the pretreatment sample but not in the posttreatment sample.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>In hematologic patients with high tumor burden, HLA homozygosity should be viewed with suspicion for potential LOH. Therefore, HLA testing should be repeated, preferably with a non\u2010hematological source (e.g., buccal swab) or following successful reduction of the tumor burden.<\/jats:p><\/jats:sec>","DOI":"10.1111\/trf.17189","type":"journal-article","created":{"date-parts":[[2022,11,25]],"date-time":"2022-11-25T08:39:07Z","timestamp":1669365547000},"page":"263-268","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Loss of heterozygosity leading to incorrect <scp>HLA<\/scp> typing for platelet\u2010transfusion refractory patient"],"prefix":"10.1111","volume":"63","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0128-9147","authenticated-orcid":false,"given":"Michael","family":"Horwath","sequence":"first","affiliation":[{"name":"Department of Pathology and Laboratory Medicine Emory University School of Medicine  Atlanta Georgia 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