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We assessed the presence of Gram\u2010negative bacteria and <jats:italic>Staphylococcus aureus<\/jats:italic> on blood donor skin and evaluated skin antisepsis in the Democratic Republic of the Congo (DRC).<\/jats:p><\/jats:sec><jats:sec><jats:title>Study Design and Methods<\/jats:title><jats:p>Among blood donors at the National Blood Transfusion Center (NBTC) and at a rural hospital, the antecubital fossa skin of the non\u2010disinfected arm (not used for blood collection) was swabbed (25cm<jats:sup>2<\/jats:sup> surface) and cultured for total and Gram\u2010negative bacterial counts. Bacteria were identified with MALDI\u2010TOF and tested for antibiotic susceptibility by disk diffusion. For evaluation of the NBTC antisepsis procedure (i.e., ethanol 70%), the culture results of the disinfected arm (used for blood collection) were compared with those of the non\u2010disinfected arm.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Median total bacterial counts on 161 studied non\u2010disinfected arms were 1065 Colony\u2010Forming Units (CFU) per 25\u2009cm<jats:sup>2<\/jats:sup>, with 43.8% (70\/160) of blood donors growing Gram\u2010negative bacteria and 3.8% (6\/159) <jats:italic>Staphylococcus aureus<\/jats:italic> (2\/6 methicillin\u2010resistant). Non\u2010fermentative Gram\u2010negative rods predominated (74\/93 isolates, majority <jats:italic>Pseudomonas<\/jats:italic> spp., <jats:italic>Acinetobacter<\/jats:italic> spp.). Enterobacterales comprised 19\/93 isolates (mostly <jats:italic>Pantoea<\/jats:italic> spp. and <jats:italic>Enterobacter<\/jats:italic> spp.), 5\/19 were multidrug\u2010resistant. In only two cases (1.9%, 2\/108) the NBTC antisepsis procedure met the acceptance criterion of \u22642\u00a0CFU\/25\u2009cm<jats:sup>2<\/jats:sup>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Skin bacterial counts and species among blood donors in DRC were similar to previously studied Caucasian populations, including cold\u2010tolerating species and bacteria previously described in transfusion reactions. Prevention of contamination (e.g., antisepsis) needs further evaluation and customization to sSA.<\/jats:p><\/jats:sec>","DOI":"10.1111\/trf.17196","type":"journal-article","created":{"date-parts":[[2022,12,7]],"date-time":"2022-12-07T17:16:19Z","timestamp":1670433379000},"page":"360-372","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Presence of Gram\u2010negative bacteria and <i>Staphylococcus aureus<\/i> on the skin of blood donors in the Democratic Republic of the Congo"],"prefix":"10.1111","volume":"63","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3930-3100","authenticated-orcid":false,"given":"Anne\u2010Sophie","family":"Heroes","sequence":"first","affiliation":[{"name":"Department of Clinical Sciences Institute of Tropical Medicine  Antwerp Belgium"},{"name":"Department of Microbiology, Immunology and Transplantation KU Leuven  Leuven 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