{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,29]],"date-time":"2026-01-29T13:54:44Z","timestamp":1769694884618,"version":"3.49.0"},"reference-count":25,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2023,1,8]],"date-time":"2023-01-08T00:00:00Z","timestamp":1673136000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100008914","name":"Advance Queensland","doi-asserted-by":"publisher","award":["Advance Queensland Industry Research Fellowship"],"award-info":[{"award-number":["Advance Queensland Industry Research Fellowship"]}],"id":[{"id":"10.13039\/501100008914","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Transfusion"],"published-print":{"date-parts":[[2023,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Severe acute respiratory syndrome coronavirus 2 (SARS\u2010CoV\u20102) is unlikely to be a major transfusion\u2010transmitted pathogen; however, convalescent plasma is a treatment option used in some regions. The risk of transfusion\u2010transmitted infections can be minimized by implementing Pathogen Inactivation (PI), such as THERAFLEX MB\u2010plasma and THERAFLEX UV\u2010Platelets systems. Here we examined the capability of these PI systems to inactivate SARS\u2010CoV\u20102.<\/jats:p><\/jats:sec><jats:sec><jats:title>Study Design and Methods<\/jats:title><jats:p>SARS\u2010CoV\u20102 spiked plasma units were treated using the THERAFLEX MB\u2010Plasma system in the presence of methylene blue (~0.8\u00a0\u03bcmol\/L; visible light doses: 20, 40, 60, and 120 [standard] J\/cm<jats:sup>2<\/jats:sup>). SARS\u2010CoV\u20102 spiked platelet concentrates (PCs) were treated using the THERAFLEX UV\u2010platelets system (UVC doses: 0.05, 0.10, 0.15, and 0.20 [standard] J\/cm<jats:sup>2<\/jats:sup>). Samples were taken prior to the first and after each illumination dose, and viral infectivity was assessed using an immunoplaque assay.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Treatment of spiked plasma with the THERAFLEX MB\u2010Plasma system resulted in an average \u22655.03 log<jats:sub>10<\/jats:sub> reduction in SARS\u2010CoV\u20102 infectivity at one third (40\u2009J\/cm<jats:sup>2<\/jats:sup>) of the standard visible light dose. For the platelet concentrates (PCs), treatment with the THERAFLEX UV\u2010Platelets system resulted in an average \u22655.18 log<jats:sub>10<\/jats:sub> reduction in SARS\u2010CoV\u20102 infectivity at the standard UVC dose (0.2\u00a0J\/cm<jats:sup>2<\/jats:sup>).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>SARS\u2010CoV\u20102 infectivity was reduced in plasma and platelets following treatment with the THERAFLEX MB\u2010Plasma and THERAFLEX UV\u2010Platelets systems, to the limit of detection, respectively. These PI technologies could therefore be an effective option to reduce the risk of transfusion\u2010transmitted emerging pathogens.<\/jats:p><\/jats:sec>","DOI":"10.1111\/trf.17238","type":"journal-article","created":{"date-parts":[[2022,12,27]],"date-time":"2022-12-27T13:58:48Z","timestamp":1672149528000},"page":"288-293","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Inactivation of <scp>SARS\u2010CoV<\/scp>\u20102 infectivity in platelet concentrates or plasma following treatment with ultraviolet C light or with methylene blue combined with visible light"],"prefix":"10.1111","volume":"63","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0139-9829","authenticated-orcid":false,"given":"Jody","family":"Hobson\u2010Peters","sequence":"first","affiliation":[{"name":"School of Chemistry and Molecular Biosciences The University of Queensland  Brisbane Queensland Australia"},{"name":"Australian Infectious Diseases Research Centre, Global Virus Network Centre of Excellence  Brisbane Queensland Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alberto A.","family":"Amarilla","sequence":"additional","affiliation":[{"name":"School of Chemistry and Molecular Biosciences The University of Queensland  Brisbane Queensland Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6385-1580","authenticated-orcid":false,"given":"Lina","family":"Rustanti","sequence":"additional","affiliation":[{"name":"Research and Development, Australian Red Cross Lifeblood  Brisbane Queensland Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3674-6934","authenticated-orcid":false,"given":"Denese C.","family":"Marks","sequence":"additional","affiliation":[{"name":"Research and Development, Australian Red Cross Lifeblood  Brisbane Queensland Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6824-5432","authenticated-orcid":false,"given":"Eileen","family":"Roulis","sequence":"additional","affiliation":[{"name":"Research and Development, Australian Red Cross Lifeblood  Brisbane Queensland Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexander A.","family":"Khromykh","sequence":"additional","affiliation":[{"name":"School of Chemistry and Molecular Biosciences The University of Queensland  Brisbane Queensland Australia"},{"name":"Australian Infectious Diseases Research Centre, Global Virus Network Centre of Excellence  Brisbane Queensland Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Naphak","family":"Modhiran","sequence":"additional","affiliation":[{"name":"School of Chemistry and Molecular Biosciences The University of Queensland  Brisbane Queensland Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniel","family":"Watterson","sequence":"additional","affiliation":[{"name":"School of Chemistry and Molecular Biosciences The University of Queensland  Brisbane Queensland Australia"},{"name":"Australian Infectious Diseases Research Centre, Global Virus Network Centre of Excellence  Brisbane Queensland Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stefan","family":"Reichenberg","sequence":"additional","affiliation":[{"name":"Macopharma International GmbH  Langen Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Frank","family":"Tolksdorf","sequence":"additional","affiliation":[{"name":"Macopharma International GmbH  Langen Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chryslain","family":"Sumian","sequence":"additional","affiliation":[{"name":"Macopharma  Tourcoing France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5858-5097","authenticated-orcid":false,"given":"Axel","family":"Seltsam","sequence":"additional","affiliation":[{"name":"Bavarian Red Cross Blood Service  Nuremberg Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9050-1295","authenticated-orcid":false,"given":"Ute","family":"Gravemann","sequence":"additional","affiliation":[{"name":"German Red Cross Blood Service NSTOB  Springe Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3446-8248","authenticated-orcid":false,"given":"Helen M.","family":"Faddy","sequence":"additional","affiliation":[{"name":"Research and Development, Australian Red Cross Lifeblood  Brisbane Queensland Australia"},{"name":"School of Health and Behavioural Sciences University of the Sunshine Coast  Sunshine Coast Queensland Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2023,1,8]]},"reference":[{"key":"e_1_2_9_2_1","unstructured":"World Health Organization.Weekly epidemiological update on COVID\u201019 \u2010 31 August 2022https:\/\/www.who.int\/publications\/m\/item\/weekly\u2010epidemiological\u2010update\u2010on\u2010covid\u201019\u2010\u2010\u201031\u2010august\u20102022"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(20)30183-5"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.2020008230"},{"key":"e_1_2_9_5_1","first-page":"9A","article-title":"Lack of infectivity of SARS\u2010CoV2 RNA+ donor plasma, In 2022 plenary, Oral, and poster abstract presentations","volume":"62","author":"Muench 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