{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T10:44:48Z","timestamp":1788345888833,"version":"build-2803163510"},"reference-count":28,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2023,1,19]],"date-time":"2023-01-19T00:00:00Z","timestamp":1674086400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100000014","name":"Canadian Blood Services","doi-asserted-by":"publisher","award":["IG2018\u2010JA"],"award-info":[{"award-number":["IG2018\u2010JA"]}],"id":[{"id":"10.13039\/501100000014","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004543","name":"China Scholarship Council","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004543","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Transfusion"],"published-print":{"date-parts":[[2023,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Circulating CD71<jats:sup>+<\/jats:sup> red blood cells (RBCs) have been reported to play an immunomodulatory role in vivo<jats:italic>,<\/jats:italic> which may contribute to adverse donor\u2013recipient sex\u2010mismatched transfusion outcomes. However, it is not clear how CD71<jats:sup>+<\/jats:sup> RBC quantity in red cell concentrates (RCCs) is affected by manufacturing methods and donor factors such as donor sex, donor age, pre\u2010donation hemoglobin (Hb), venous Hb (Hb<jats:sub>v<\/jats:sub>) levels, and donation frequency.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We determined CD71<jats:sup>+<\/jats:sup> RBCs and Hb levels in whole blood (WB) from healthy donors (42 male\/38 female). Using small\u2010scale red cell filtration (RCF) and whole blood filtration (WBF) methods, leukoreduced RCCs were processed from WB samples (<jats:italic>n<\/jats:italic>\u00a0=\u20096) and the CD71<jats:sup>+<\/jats:sup> RBCs were determined at days 1, 7, and 28. We examined uni\u2010 and multivariate associations among CD71<jats:sup>+<\/jats:sup> RBCs, donor factors, and manufacturing method.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Male donors had a higher CD71<jats:sup>+<\/jats:sup> RBC concentration than females (<jats:italic>p<\/jats:italic>\u00a0&lt;\u2009.001), especially male donors aged 17\u201350\u2009years with 1 or 2 WB donations over the previous 12\u2009months. Donors with a Hb<jats:sub>v<\/jats:sub> above 155\u2009g\/L had a higher CD71<jats:sup>+<\/jats:sup> RBC concentration than an Hb<jats:sub>v<\/jats:sub> level below 140\u2009g\/L (<jats:italic>p<\/jats:italic>\u00a0&lt;\u2009.05). There was a positive correlation between pre\u2010donation Hb and CD71<jats:sup>+<\/jats:sup> RBC concentration (Pearson <jats:italic>r<\/jats:italic>\u00a0=\u20090.41). WBF RCCs had a higher total number of CD71<jats:sup>+<\/jats:sup> RBCs than RCF\u2010produced RCCs on day 1 (<jats:italic>p<\/jats:italic>\u00a0&lt;\u2009.05).<\/jats:p><\/jats:sec><jats:sec><jats:title>Discussion<\/jats:title><jats:p>RCCs have variable numbers of CD71<jats:sup>+<\/jats:sup> RBCs. This makes understanding the impact of donor factors and manufacturing methods on the immunomodulatory effect of CD71<jats:sup>+<\/jats:sup> RBCs critical in exploring donor\u2013recipient sex\u2010mismatched transfusions.<\/jats:p><\/jats:sec>","DOI":"10.1111\/trf.17250","type":"journal-article","created":{"date-parts":[[2023,1,19]],"date-time":"2023-01-19T12:24:44Z","timestamp":1674131084000},"page":"601-609","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Donor sex, <scp>pre\u2010donation<\/scp> hemoglobin, and manufacturing affect <scp>CD71<\/scp><sup>+<\/sup><scp>cells<\/scp> in red cell concentrates"],"prefix":"10.1111","volume":"63","author":[{"given":"Wenhui","family":"Li","sequence":"first","affiliation":[{"name":"Department of Laboratory Medicine and Pathology University of Alberta  Edmonton 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