{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,15]],"date-time":"2026-05-15T01:25:15Z","timestamp":1778808315372,"version":"3.51.4"},"reference-count":25,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2023,2,8]],"date-time":"2023-02-08T00:00:00Z","timestamp":1675814400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Transfusion"],"published-print":{"date-parts":[[2023,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>A large, worldwide outbreak of mpox (formerly referred to as monkeypox) involving mainly men who have sex with men commenced in May 2022. We evaluated the frequency of positivity for the causative agent, monkeypox virus (MPXV), in blood donations collected in August 2022, during the outbreak period in Southern England.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods\/Materials<\/jats:title><jats:p>The sensitivity and specificity of an MPXV\u2010specific PCR and a generic non\u2010variola orthopoxvirus (NVO) PCR were evaluated using samples from mpox cases and synthetic DNA standards. Residual minipools from nucleic acid testing were obtained from 10,896 blood donors in Southern England, with 21% from London.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>MPXV and NVO PCRs were both capable of detection of single copies of target sequence with calculated limits of detection (LOD)<jats:sub>90<\/jats:sub>\u2009s of 2.3 and 2.1 DNA copies and analytical sample sensitivities of 46 and 42 MPXV DNA copies\/ml, respectively. 454 minipools produced from 10,896 unique donors were assayed for MPXV DNA by both methods. No positive minipools were detected by either PCR.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Although blood donors are unrepresentative of the UK population in terms of MPXV infection risk, the uniformly negative MPXV DNA testing results provide reassurance that MPXV viraemia and potential transmission risk were rare or absent in donors during the outbreak period. Minipools from blood donors allow rapid implementation of large\u2010scale population\u2010based screening for emerging pathogens and represent an important resource for pandemic preparedness.<\/jats:p><\/jats:sec>","DOI":"10.1111\/trf.17266","type":"journal-article","created":{"date-parts":[[2023,2,8]],"date-time":"2023-02-08T09:19:50Z","timestamp":1675847990000},"page":"690-695","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Absence of detectable monkeypox virus <scp>DNA<\/scp> in 11,000 English blood donations during the 2022 outbreak"],"prefix":"10.1111","volume":"63","author":[{"given":"Chanice","family":"Knight","sequence":"first","affiliation":[{"name":"Nuffield Department of Medicine, Peter Medawar Building for Pathogen Research University of Oxford  Oxford UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Julien","family":"Andreani","sequence":"additional","affiliation":[{"name":"Nuffield Department of Medicine, Peter Medawar Building for Pathogen Research University of Oxford  Oxford UK"},{"name":"Centre Hospitalier Universitaire (CHU) Grenoble\u2010Alpes  Grenoble France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Neil","family":"Garrett","sequence":"additional","affiliation":[{"name":"NHSBT Filton Testing\/Blood Supply  Bristol UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mark","family":"Winter","sequence":"additional","affiliation":[{"name":"NHSBT Filton Testing\/Blood Supply  Bristol UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tanya","family":"Golubchik","sequence":"additional","affiliation":[{"name":"Sydney Infectious Diseases Institute Faculty of Medicine and Health, University of Sydney  Sydney Australia"},{"name":"Big Data Institute, Nuffield Department of Medicine  Oxford UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Judy","family":"Breuer","sequence":"additional","affiliation":[{"name":"Division of Infection and Immunity University College London  London UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Claire","family":"Reynolds","sequence":"additional","affiliation":[{"name":"NHS Blood and Transplant\/UK Health Security Agency Epidemiology Unit, NHSBT  London UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Susan R.","family":"Brailsford","sequence":"additional","affiliation":[{"name":"NHS Blood and Transplant\/UK Health Security Agency Epidemiology Unit, NHSBT  London UK"},{"name":"Microbiology Services National Health Service (NHS) Blood and Transplant  London UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9154-4190","authenticated-orcid":false,"given":"Heli","family":"Harvala","sequence":"additional","affiliation":[{"name":"Division of Infection and Immunity University College London  London UK"},{"name":"Microbiology Services National Health Service (NHS) Blood and Transplant  London UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7964-4700","authenticated-orcid":false,"given":"Peter","family":"Simmonds","sequence":"additional","affiliation":[{"name":"Nuffield Department of Medicine, Peter Medawar Building for Pathogen Research University of Oxford  Oxford UK"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2023,2,8]]},"reference":[{"key":"e_1_2_8_2_1","volume-title":"WHO recommends new name for monkeypox disease [monograph on the internet]","year":"2022"},{"key":"e_1_2_8_3_1","unstructured":"Investigation into monkeypox outbreak in England: technical briefing 8 [monograph on the internet].2022. Available from:https:\/\/www.gov.uk\/government\/publications\/monkeypox\u2010outbreak\u2010technical\u2010briefings\/investigation\u2010into\u2010monkeypox\u2010outbreak\u2010in\u2010england\u2010technical\u2010briefing\u20108"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/156.2.293"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.o1239"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.2807\/1560-7917.ES.2022.27.36.2200620"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.17100"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/tme.12918"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(22)00228-6"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.3390\/diagnostics12092200"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jviromet.2010.07.012"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcv.2006.03.012"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/bjh.18388"},{"key":"e_1_2_8_14_1","volume-title":"Lab alert: MPXV TNF receptor gene deletion may Lead to false negative results with some MPXV specific LDTs [monograph on the internet]","year":"2022"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.30894"},{"key":"e_1_2_8_16_1","unstructured":"2022 Outbreak Cases and Data [monograph on the internet].2022. Available from:https:\/\/www.cdc.gov\/poxvirus\/monkeypox\/response\/2022\/index.html"},{"key":"e_1_2_8_17_1","unstructured":"Joint ECDC\u2010WHO Regional Office for Europe Monkeypox Surveillance Bulletin [monograph on the internet]2022. Available from:https:\/\/monkeypoxreport.ecdc.europa.eu"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMc062788"},{"key":"e_1_2_8_19_1","unstructured":"Monkeypox [monograph on the internet].2022. 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