{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,7]],"date-time":"2025-10-07T05:08:58Z","timestamp":1759813738846,"version":"3.40.5"},"reference-count":22,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2023,3,31]],"date-time":"2023-03-31T00:00:00Z","timestamp":1680220800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Transfusion"],"published-print":{"date-parts":[[2023,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aims<\/jats:title><jats:p>The aim was to define the source of contamination of cryoprecipitate intercepted during visual inspection before transfusion.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and Methods<\/jats:title><jats:p>A clot was observed in one unit of cryoprecipitate before blood transfusion at the Dongyang People's Hospital. Bacterial cultures were performed using the BacT\/ALERT system (BacT\/ALERT 3D, bioMerieux, Durham, NC). The isolated bacteria were identified through conventional biochemical identification, matrix\u2010assisted laser desorption ionization\u2010time of flight mass spectrometry, and molecular analysis based on 16sr RNA. Samples from all individuals who came into direct contact with the cryoprecipitate were cultured, and the positive samples were then referred for bacterial identification.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>A leak was found at the edge of a blood bag containing the cryoprecipitate. <jats:italic>Cupriavidus paucula<\/jats:italic> was identified both in the cryoprecipitate and water from the water bath. However, there was no growth of <jats:italic>C. paucula<\/jats:italic> in the samples obtained from the red blood cell suspension co\u2010component, puncture site of the blood donor, blood storage refrigerator, transport case, and centrifuge.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p><jats:italic>C. paucula<\/jats:italic> in the water from the water bath contaminated the cryoprecipitate through the invisible slit in the blood bag during thawing. Regular disinfection of water baths, double\u2010bagging of blood products during thawing, and careful screening of blood products before transfusion should be performed to prevent the transfusion of contaminated cryoprecipitate.<\/jats:p><\/jats:sec>","DOI":"10.1111\/trf.17337","type":"journal-article","created":{"date-parts":[[2023,3,31]],"date-time":"2023-03-31T11:54:28Z","timestamp":1680263668000},"page":"1246-1249","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Cryoprecipitate contaminated with <i>Cupriavidus pauculus<\/i>: A rare cautionary case report"],"prefix":"10.1111","volume":"63","author":[{"given":"Wenxin","family":"Li","sequence":"first","affiliation":[{"name":"Dongyang People's Hospital  Dongyang 450000 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5086-294X","authenticated-orcid":false,"given":"Yongjun","family":"Wang","sequence":"additional","affiliation":[{"name":"Blood Center of Zhejiang Province Key Laboratory of Blood Safety Research of Zhejiang Province  Hangzhou 310052 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wenjuan","family":"Han","sequence":"additional","affiliation":[{"name":"Blood Center of Zhejiang Province Key Laboratory of Blood Safety Research of Zhejiang Province  Hangzhou 310052 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guomei","family":"Yin","sequence":"additional","affiliation":[{"name":"Blood Center of Zhejiang Province Key Laboratory of Blood Safety Research of Zhejiang Province  Hangzhou 310052 China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2023,3,31]]},"reference":[{"key":"e_1_2_5_2_1","unstructured":"FDA.Fatalities reported to FDA following blood collection and transfusion annual summary for fiscal year 2016.2016[cited 2020 Mar 25]. Available from:https:\/\/www.fda.gov\/downloads\/BiologicsBloodVaccines\/SafetyAvailability\/ReportaProblem\/TransfusionDonationFatalities\/UCM598243.pdf"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.14202"},{"key":"e_1_2_5_4_1","first-page":"713","article-title":"Enlargement of the WHO international repository for platelet transfusion\u2010relevant bacterial reference strains","volume":"112","author":"Spindler\u2010Raffel E","year":"2017","journal-title":"Transfusion"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2012.03763.x"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1515\/CCLM.2008.151"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.14148"},{"key":"e_1_2_5_8_1","first-page":"348","article-title":"Bacterial contamination of blood products for transfusion in The Democratic Republic of the Congo: temperature monitoring, qualitative and semi\u2010quantitative culture","volume":"18","author":"Heroes AS","year":"2020","journal-title":"Blood Transfus"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01874-09"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhin.2011.02.008"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tmrv.2005.05.002"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1097\/MAT.0000000000000120"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3148.2009.00948.x"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13054-018-2212-9"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.15180"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2015-07-655944"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1099\/ijs.0.63247-0"},{"key":"e_1_2_5_18_1","doi-asserted-by":"publisher","DOI":"10.1086\/425368"},{"key":"e_1_2_5_19_1","first-page":"116","article-title":"Evaluation of the sensitivity and specificity of use of glucose and pH for bacterial screening of platelet concentrates compared to the bact\/alert","volume":"33","author":"Razjou F","year":"2017","journal-title":"Transfus Med Hemother"},{"key":"e_1_2_5_20_1","doi-asserted-by":"publisher","DOI":"10.1159\/000499349"},{"key":"e_1_2_5_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.16210"},{"key":"e_1_2_5_22_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.15951"},{"key":"e_1_2_5_23_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.16776"}],"container-title":["Transfusion"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/trf.17337","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,5,3]],"date-time":"2024-05-03T06:52:36Z","timestamp":1714719156000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/trf.17337"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,3,31]]},"references-count":22,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2023,6]]}},"alternative-id":["10.1111\/trf.17337"],"URL":"https:\/\/doi.org\/10.1111\/trf.17337","archive":["Portico"],"relation":{},"ISSN":["0041-1132","1537-2995"],"issn-type":[{"type":"print","value":"0041-1132"},{"type":"electronic","value":"1537-2995"}],"subject":[],"published":{"date-parts":[[2023,3,31]]},"assertion":[{"value":"2022-10-12","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-03-15","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-03-31","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}