{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,25]],"date-time":"2026-06-25T04:44:24Z","timestamp":1782362664264,"version":"3.54.5"},"reference-count":29,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2023,6,30]],"date-time":"2023-06-30T00:00:00Z","timestamp":1688083200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100000014","name":"Canadian Blood Services","doi-asserted-by":"publisher","award":["IG2018\u2010JA"],"award-info":[{"award-number":["IG2018\u2010JA"]}],"id":[{"id":"10.13039\/501100000014","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100010481","name":"Etablissement Fran\u00e7ais du Sang","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100010481","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Transfusion"],"published-print":{"date-parts":[[2023,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Blood donors at the extremes of the age spectrum (16\u201319\u2009years vs. \u226575\u2009years) are characterized by increased risks of iron deficiency and anemia, and are often underrepresented in studies evaluating the effects of donor characteristics on red blood cells (RBC) transfusion effectiveness. The aim of this study was to conduct quality assessments of RBC concentrates from these unique age groups.<\/jats:p><\/jats:sec><jats:sec><jats:title>Study design<\/jats:title><jats:p>We characterized 150 leukocyte\u2010reduced (LR)\u2010RBCs units from 75 teenage donors, who were matched by sex, and ethnicity with 75 older donors. LR\u2010RBC units were manufactured at three large blood collection centers in the USA and Canada. Quality assessments included storage hemolysis, osmotic hemolysis, oxidative hemolysis, osmotic gradient ektacytometry, hematological indices, and RBC bioactivity.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>RBC concentrates from teenage donors had smaller (9%) mean corpuscular volume and higher (5%) RBC concentration compared with older donors counterparts. Stored RBCs from teenage donors exhibited increased susceptibility to oxidative hemolysis (&gt;2\u2010fold) compared with RBCs from older donors. This was observed at all testing centers independent of sex, storage duration, or the type of additive solution. RBCs from teenage male donors had increased cytoplasmatic viscosity and lower hydration compared with older donor RBCs. Evaluations of RBC supernatant bioactivity suggested that donor age was not associated with altered expression of inflammatory markers (CD31, CD54, and IL\u20106) on endothelial cells.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The reported findings are likely intrinsic to RBCs and reflect age\u2010specific changes in RBC antioxidant capacity and physical characteristics that may impact RBC survival during cold storage and after transfusion.<\/jats:p><\/jats:sec>","DOI":"10.1111\/trf.17471","type":"journal-article","created":{"date-parts":[[2023,6,30]],"date-time":"2023-06-30T12:59:44Z","timestamp":1688129984000},"page":"1506-1518","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["Quality assessment of red blood cell concentrates from blood donors at the extremes of the age spectrum: The <scp>BEST<\/scp> collaborative study"],"prefix":"10.1111","volume":"63","author":[{"given":"Marc","family":"Cloutier","sequence":"first","affiliation":[{"name":"Affaires M\u00e9dicales et Innovation H\u00e9ma\u2010Qu\u00e9bec  Qu\u00e9bec Qu\u00e9bec Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8041-928X","authenticated-orcid":false,"given":"Fabrice","family":"Cognasse","sequence":"additional","affiliation":[{"name":"Research Department, F\u201042023 \u00c9tablissement Fran\u00e7ais du Sang Auvergne\u2010Rh\u00f4ne\u2010Alpes  Saint\u2010\u00c9tienne France"},{"name":"INSERM, U 1059 SAINBIOSE Universit\u00e9 Jean Monnet Saint\u2010\u00c9tienne, Mines Saint Etienne, F\u201042023  Saint\u2010Etienne France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana Paula Hitomi","family":"Yokoyama","sequence":"additional","affiliation":[{"name":"Hemotherapy and Cell Therapy Department Hospital Israelita Albert Einstein  S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kelsey","family":"Hazegh","sequence":"additional","affiliation":[{"name":"Vitalant Research Institute  Denver Colorado USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8854-2872","authenticated-orcid":false,"given":"Olga","family":"Mykhailova","sequence":"additional","affiliation":[{"name":"Innovation and Portfolio Management, Canadian Blood Services  Edmonton Alberta Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mackenzie","family":"Brandon\u2010Coatham","sequence":"additional","affiliation":[{"name":"Innovation and Portfolio Management, Canadian Blood Services  Edmonton Alberta Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hind","family":"Hamzeh\u2010Cognasse","sequence":"additional","affiliation":[{"name":"Research Department, F\u201042023 \u00c9tablissement Fran\u00e7ais du Sang Auvergne\u2010Rh\u00f4ne\u2010Alpes  Saint\u2010\u00c9tienne France"},{"name":"INSERM, U 1059 SAINBIOSE Universit\u00e9 Jean Monnet Saint\u2010\u00c9tienne, Mines Saint Etienne, F\u201042023  Saint\u2010Etienne France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jose Mauro","family":"Kutner","sequence":"additional","affiliation":[{"name":"Hemotherapy and Cell Therapy Department Hospital Israelita Albert Einstein  S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1445-827X","authenticated-orcid":false,"given":"Jason P.","family":"Acker","sequence":"additional","affiliation":[{"name":"Innovation and Portfolio Management, Canadian Blood Services  Edmonton Alberta Canada"},{"name":"Department of Laboratory Medicine and Pathology University of Alberta  Edmonton Alberta Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6558-0913","authenticated-orcid":false,"given":"Tamir","family":"Kanias","sequence":"additional","affiliation":[{"name":"Vitalant Research Institute  Denver Colorado USA"},{"name":"Department of Pathology University of Colorado Denver Anschutz Medical Campus  Aurora Colorado USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"name":"the Biomedical Excellence for Safer Transfusion (BEST) Collaborative","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,6,30]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1182\/bloodadvances.2017004820"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.13745"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.3324\/haematol.2020.246603"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1001\/jamanetworkopen.2021.23942"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1001\/jamainternmed.2017.0914"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1001\/jamainternmed.2017.0890"},{"key":"e_1_2_8_8_1","doi-asserted-by":"crossref","DOI":"10.1542\/peds.2016-2955","article-title":"Teenage blood donors: are we asking too little and taking too much?","volume":"139","author":"Bloch 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