{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,24]],"date-time":"2026-07-24T13:26:24Z","timestamp":1784899584340,"version":"3.55.0"},"reference-count":14,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2023,9,5]],"date-time":"2023-09-05T00:00:00Z","timestamp":1693872000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["20.K03.HBL"],"award-info":[{"award-number":["20.K03.HBL"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["HL13950"],"award-info":[{"award-number":["HL13950"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["T32 HL139425"],"award-info":[{"award-number":["T32 HL139425"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000738","name":"U.S. Department of Veterans Affairs","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000738","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Transfusion"],"published-print":{"date-parts":[[2023,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Introduction<\/jats:title><jats:p>Warm antibody\u2010mediated autoimmune hemolysis (WAIHA) is most often due to immunoglobulin G (IgG) antibodies and is detected by direct antiglobulin test (DAT). However, about 10% cases of hemolytic anemia are DAT negative. Herein, we describe a patient with DAT\u2010negative hemolytic anemia due to an anti\u2010IgA antibody. We investigate if isolated IgA can promote erythrophagocytosis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We isolated patient and control IgA on Jacalin agarose. Isolated IgA was used to sensitize healthy ABO\/Rh\u2010matched donor red blood cells (RBC). Antibody binding was examined by flowcytometry. The effect of IgA on erythrophagocytosis was evaluated using Macrophage colony stimulating factor 1 (M\u2010CSF)\u2010differentiated autologous macrophages by Giemsa staining and immunofluorescence microscopy.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>We show that isolated IgA from the serum can bind to red cells. In addition, RBCs were phagocytosed efficiently by autologous macrophages in the presence of patient IgA.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Our results show that IgA antibodies are capable of inducing erythrophagocytosis like IgG antibodies in the absence of complement fixation.<\/jats:p><\/jats:sec>","DOI":"10.1111\/trf.17532","type":"journal-article","created":{"date-parts":[[2023,9,5]],"date-time":"2023-09-05T10:33:50Z","timestamp":1693910030000},"page":"1978-1982","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Erythrophagocytosis in autoimmune immunoglobulin <scp>A<\/scp>\u2010mediated hemolysis"],"prefix":"10.1111","volume":"63","author":[{"given":"Jahnavi","family":"Gollamudi","sequence":"first","affiliation":[{"name":"Department of Internal Medicine, Division of Hematology University of Cincinnati  Cincinnati Ohio USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Swapan Kumar","family":"Dasgupta","sequence":"additional","affiliation":[{"name":"Department of Pathology and Medicine Baylor College of Medicine, Center for Translational Research on Inflammatory Diseases (CTRID), Michael E. DeBakey Veterans Affairs Medical Center  Houston Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2186-7036","authenticated-orcid":false,"given":"Perumal","family":"Thiagarajan","sequence":"additional","affiliation":[{"name":"Department of Pathology and Medicine Baylor College of Medicine, Center for Translational Research on Inflammatory Diseases (CTRID), Michael E. DeBakey Veterans Affairs Medical Center  Houston Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,9,5]]},"reference":[{"key":"e_1_2_7_2_1","volume-title":"Immune hemolytic anemias","author":"Petz LD","year":"2004"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3148.2008.00891.x"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1309\/CJAW6N8J6H0HR2WM"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/0090-1229(81)90213-0"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2010-03-276162"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V64.5.1000.1000"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bcmd.2013.12.003"},{"key":"e_1_2_7_9_1","article-title":"Purification of human IgA","volume":"38","author":"Pack TD","year":"2001","journal-title":"Current Protocols in Immunology"},{"issue":"10","key":"e_1_2_7_10_1","article-title":"In vitro macrophage\u2010mediated phagocytosis assay of brain tumors","volume":"12","author":"Gardner M","year":"2020","journal-title":"Cureus"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00930-19"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj2710285"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2020.01141"},{"issue":"4","key":"e_1_2_7_14_1","first-page":"423","article-title":"Structure and function of human IgA Fc receptors (Fc alpha R)","volume":"16","author":"Morton HC","year":"1996","journal-title":"Crit Rev Immunol"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.162.4.2146"}],"container-title":["Transfusion"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/trf.17532","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,4]],"date-time":"2023-10-04T09:09:37Z","timestamp":1696410577000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/trf.17532"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,9,5]]},"references-count":14,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2023,10]]}},"alternative-id":["10.1111\/trf.17532"],"URL":"https:\/\/doi.org\/10.1111\/trf.17532","archive":["Portico"],"relation":{},"ISSN":["0041-1132","1537-2995"],"issn-type":[{"value":"0041-1132","type":"print"},{"value":"1537-2995","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,9,5]]},"assertion":[{"value":"2023-05-17","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-07-31","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-09-05","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}