{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,6]],"date-time":"2026-05-06T13:44:50Z","timestamp":1778075090372,"version":"3.51.4"},"reference-count":21,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2023,11,3]],"date-time":"2023-11-03T00:00:00Z","timestamp":1698969600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Transfusion"],"published-print":{"date-parts":[[2023,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Accurate antibody titration is crucial in prenatal evaluations to identify patients who need clinical monitoring for hemolytic disease of the fetus and newborn (HDFN) causing fetal anemia. This study compares the established gold standard method of manual tube saline indirect antiglobulin testing (SIAT) with the newer automated solid phase (ASP) method of antibody titration and aims to establish the critical titer threshold for ASP that corresponds to the previously established SIAT critical threshold of \u226516 used in our laboratory.<\/jats:p><\/jats:sec><jats:sec><jats:title>Study Design and Methods<\/jats:title><jats:p>One hundred fifty\u2010seven prenatal and donor plasma samples with known antibodies were tested using both SIAT and ASP methodologies and results were compared.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The study found that ASP titers were, on average, 1.33 dilutions higher than SIAT titers. The critical titer cutoff for ASP was determined to be \u226532, which is one tube higher than the SIAT cutoff of \u226516.<\/jats:p><\/jats:sec><jats:sec><jats:title>Discussion<\/jats:title><jats:p>The ASP method for antibody titration offers greater reproducibility and efficiency compared with manual SIAT titration. This study suggests that a titer cutoff of \u226532 is appropriate for most clinically significant antibodies using ASP. However, further research is needed to determine the comparability of ASP with SIAT in samples with multiple antibodies, anti\u2010M antibodies, and other less common antibodies. Validation of the ASP titer cutoff against HDFN clinical outcomes is required before implementing this test for routine use in perinatal antibody titration.<\/jats:p><\/jats:sec>","DOI":"10.1111\/trf.17571","type":"journal-article","created":{"date-parts":[[2023,11,3]],"date-time":"2023-11-03T09:48:10Z","timestamp":1699004890000},"page":"2289-2296","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Comparison of automated solid phase versus manual saline indirect antiglobulin test methodology for non\u2010<scp>ABO<\/scp> antibody titration: Implications for perinatal antibody monitoring"],"prefix":"10.1111","volume":"63","author":[{"ORCID":"https:\/\/orcid.org\/0009-0008-6066-1632","authenticated-orcid":false,"given":"Shuang","family":"Niu","sequence":"first","affiliation":[{"name":"Department of Laboratory Medicine and Pathology University of Alberta  Edmonton Alberta Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Megan","family":"Vetsch","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine and Pathology University of Alberta  Edmonton Alberta Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lynnette","family":"Beaudin","sequence":"additional","affiliation":[{"name":"Laboratory Services Canadian Blood Services  Edmonton Alberta Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Melanie","family":"Bodnar","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine and Pathology University of Alberta  Edmonton Alberta Canada"},{"name":"Laboratory Services Canadian Blood Services  Edmonton Alberta Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7738-3378","authenticated-orcid":false,"given":"Gwen","family":"Clarke","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine and Pathology University of Alberta  Edmonton Alberta Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2023,11,3]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1537-2995.2001.41111445.x"},{"key":"e_1_2_9_3_1","first-page":"184","article-title":"Alloimmune red blood cell antibodies: prevalence and pathogenicity in a Canadian prenatal population","volume":"37","author":"Zwingerman R","year":"2014","journal-title":"J Obstet Gynaecol Can"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/aogs.14261"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/tme.12299"},{"key":"e_1_2_9_6_1","volume-title":"Guidelines for prenatal and perinatal immunohematology","author":"Judd WJ","year":"2005"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.5858\/arpa.2015-0351-OA"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2012.03793.x"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.3109\/10408368509165789"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.transci.2012.03.018"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.21307\/immunohematology-2019-713"},{"key":"e_1_2_9_12_1","first-page":"5","article-title":"Comparison of automated and manual alloantibody titration using capture, gel, and tube. 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