{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T16:24:16Z","timestamp":1792081456184,"version":"4.4.0"},"reference-count":24,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2023,12,28]],"date-time":"2023-12-28T00:00:00Z","timestamp":1703721600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Transfusion"],"published-print":{"date-parts":[[2024,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Due to unique technical challenges, effective peripheral blood stem cell collections (PBSCs) have not been consistently reported in patients weighing less than 5\u2009kg. We describe three PBSCs performed in a 4.6\u2010kg child undergoing myeloablative chemotherapy for high\u2010grade glioma.<\/jats:p><\/jats:sec><jats:sec><jats:title>Study Design and Methods<\/jats:title><jats:p>A multidisciplinary group representing the clinical and apheresis teams adapted a PBSC protocol to accommodate the patient's size and collection targets. Special considerations included timing of the collection relative to chemotherapy, vascular access, strategies for monitoring adverse events during collection, and contingencies.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results and Discussion<\/jats:title><jats:p>The patient underwent three PBSC procedures over 2\u2009days due to suboptimal collection after the first two procedures. For procedure 1, a conservative inlet: anticoagulant (AC) ratio and AC infusion rate of 15 and 0.6\u2009mL\/min\/L total blood volume (TBV) resulted in premature discontinuation due to clotting. A ratio of 8 and AC infusion rate of 1.5\u20131.7\u2009mL\/min\/L TBV with subsequent titration to higher levels were adopted for the second and third procedures. These changes resulted in greater acid\u2010citrate\u2010dextrose exposure, that was managed by continuous calcium chloride infusion. There was no hypocalcemia, hypotension, or distress during any procedure. A total of 15\u2009\u00d7\u200910<jats:sup>6<\/jats:sup> CD34+ cells\/kg were collected. This retrospective review illustrates that PBSC can be safely undertaken in children weighing less than 5\u2009kg.<\/jats:p><\/jats:sec>","DOI":"10.1111\/trf.17687","type":"journal-article","created":{"date-parts":[[2023,12,29]],"date-time":"2023-12-29T06:39:40Z","timestamp":1703831980000},"page":"400-405","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Effective peripheral blood stem cell collection in a 4.6\u2010kg child"],"prefix":"10.1111","volume":"64","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7246-4486","authenticated-orcid":false,"given":"Jennifer M.","family":"Jones","sequence":"first","affiliation":[{"name":"Department of Pathology University of Pittsburgh Medical Center  Pittsburgh Pennsylvania USA"},{"name":"Vitalant Specialty Lab and Therapeutics  Pittsburgh Pennsylvania USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joseph E.","family":"Kiss","sequence":"additional","affiliation":[{"name":"Vitalant Specialty Lab and Therapeutics  Pittsburgh Pennsylvania USA"},{"name":"Department of Medicine University of Pittsburgh Medical Center  Pittsburgh Pennsylvania USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Irina","family":"Chibisov","sequence":"additional","affiliation":[{"name":"Department of Pathology University of Pittsburgh Medical Center  Pittsburgh Pennsylvania USA"},{"name":"Vitalant Specialty Lab and Therapeutics  Pittsburgh Pennsylvania USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paulina","family":"Horvei","sequence":"additional","affiliation":[{"name":"Division of Blood and Marrow Transplantation and Cellular Therapies University of Pittsburgh Medical Center Children's Hospital of Pittsburgh  Pittsburgh Pennsylvania USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Heather","family":"Stanczak","sequence":"additional","affiliation":[{"name":"Division of Blood and Marrow Transplantation and Cellular Therapies University of Pittsburgh Medical Center Children's Hospital of Pittsburgh  Pittsburgh Pennsylvania USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carol","family":"Burda","sequence":"additional","affiliation":[{"name":"Vitalant Specialty Lab and Therapeutics  Pittsburgh Pennsylvania USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Clark","sequence":"additional","affiliation":[{"name":"Vitalant Specialty Lab and Therapeutics  Pittsburgh Pennsylvania USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joan","family":"Sevcik","sequence":"additional","affiliation":[{"name":"Vitalant Specialty Lab and Therapeutics  Pittsburgh Pennsylvania USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carolyn","family":"Kilonsky","sequence":"additional","affiliation":[{"name":"Vitalant Specialty Lab and Therapeutics  Pittsburgh Pennsylvania USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8746-0549","authenticated-orcid":false,"given":"Alesia","family":"Kaplan","sequence":"additional","affiliation":[{"name":"Department of Pathology University of Pittsburgh Medical Center  Pittsburgh Pennsylvania USA"},{"name":"Vitalant Specialty Lab and Therapeutics  Pittsburgh Pennsylvania USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,12,28]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/jca.21465"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2007.01405.x"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/jca.21863"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/petr.13602"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/jca.21713"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/jca.22021"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pediatrneurol.2015.03.019"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbmt.2003.12.050"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1083-8791(03)80013-7"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/jca.20195"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/jca.22035"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.transci.2020.102987"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/jca.21375"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2004.00668.x"},{"key":"e_1_2_7_16_1","unstructured":"Apheresis Principles and Practice 4th edition Volume 1: Therapeutic Apheresis \u2013 Digital [Internet].aabb.org. 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