{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,16]],"date-time":"2026-05-16T17:52:29Z","timestamp":1778953949972,"version":"3.51.4"},"reference-count":19,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2024,1,18]],"date-time":"2024-01-18T00:00:00Z","timestamp":1705536000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Transfusion"],"published-print":{"date-parts":[[2024,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Antibodies to Gerbich blood group antigens are exceedingly rare and can cause moderate transfusion reactions. Several deletional variants of the <jats:italic>GE<\/jats:italic>\u2010gene, that harbors long sequence repeats, enable alloimmunization and formation of naturally occurring antibodies.<\/jats:p><\/jats:sec><jats:sec><jats:title>Subject and Methods<\/jats:title><jats:p>A female blood donor and soldier of the German Army without history of pregnancy or transfusion showed an antibody reactive with all test cells except for GE:\u20102\u20103 RBC. Thus, anti\u2010Ge2 was suspected. Molecular analysis including fragment length specific PCR, Sanger sequencing and NGS should reveal the molecular background of the deficiency. Segregation of the variant alleles should be demonstrated by family analysis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Compound heterozygosity for <jats:italic>GYPC<\/jats:italic> exon 2 (<jats:italic>GE*01.\u201002<\/jats:italic>) and exon 3 (<jats:italic>GE*01.\u201003<\/jats:italic>) deletion was detected in the donor and her sister. The mother had one exon 3 amplicon of reduced length, while the father heterozygously exhibited a truncated <jats:italic>GYPC<\/jats:italic> exon 2. NGS clearly demonstrated reduced coverages within the deletional fragments within each family member. The donor and her sister showed the complete absence of a 640\u2009bp fragment.<\/jats:p><\/jats:sec><jats:sec><jats:title>Discussion and Conclusion<\/jats:title><jats:p>Rare GE deletion variants can induce naturally occurring anti\u2010Ge2 in Caucasians. Because of an enhanced risk of injury as soldier autologous RBC of the donor were cryopreserved. The donor and her sibling can give blood for each other because of identical ABO, Rh, and K antigen blood types.<\/jats:p><\/jats:sec>","DOI":"10.1111\/trf.17711","type":"journal-article","created":{"date-parts":[[2024,1,18]],"date-time":"2024-01-18T15:14:07Z","timestamp":1705590847000},"page":"406-411","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Compound heterozygosity induces a rare Gerbich\u2010negative phenotype in an immunized blood donor"],"prefix":"10.1111","volume":"64","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5396-2954","authenticated-orcid":false,"given":"Sarah","family":"Petermann","sequence":"first","affiliation":[{"name":"Laboratory for Immunogenetics \/ HLA German Red Cross Blood Service Rhineland\u2010Palatinate and Saarland gGmbH  Bad Kreuznach Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vanessa","family":"Mayer","sequence":"additional","affiliation":[{"name":"Laboratory for Immunogenetics \/ HLA German Red Cross Blood Service Rhineland\u2010Palatinate and Saarland gGmbH  Bad Kreuznach Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexander","family":"Carbol","sequence":"additional","affiliation":[{"name":"Laboratory for Immunogenetics \/ HLA German Red Cross Blood Service Rhineland\u2010Palatinate and Saarland gGmbH  Bad Kreuznach Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andreas","family":"Opitz","sequence":"additional","affiliation":[{"name":"Laboratory for Immunogenetics \/ HLA German Red Cross Blood Service Rhineland\u2010Palatinate and Saarland gGmbH  Bad Kreuznach Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5485-2645","authenticated-orcid":false,"given":"Brigitte K.","family":"Flesch","sequence":"additional","affiliation":[{"name":"Laboratory for Immunogenetics \/ HLA German Red Cross Blood Service Rhineland\u2010Palatinate and Saarland gGmbH  Bad Kreuznach Germany"},{"name":"Laboratory for Immunogenetics \/ HLA German Red Cross Blood Service West gGmbH  Hagen Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,1,18]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.21307\/immunohematology-2019-204"},{"key":"e_1_2_9_3_1","unstructured":"ISBT.Names for GE (ISBT 020) Blood Group Alleles [monograph on the internet].2021Available 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