{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,16]],"date-time":"2026-05-16T12:19:40Z","timestamp":1778933980865,"version":"3.51.4"},"reference-count":44,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2024,2,28]],"date-time":"2024-02-28T00:00:00Z","timestamp":1709078400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Transfusion"],"published-print":{"date-parts":[[2024,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>In patients with relapsed or refractory B cell acute lymphoblastic leukemia or B cell non\u2010Hodgkin lymphoma (r\/r B\u2010ALL\/B\u2010NHL) with low CD3<jats:sup>+<\/jats:sup> cells in the peripheral blood (PB), sufficient CD3<jats:sup>+<\/jats:sup> cell yield in a single day may not be obtained with normal\u2010volume leukapheresis (NVL). Large\u2010volume leukapheresis (LVL) refers to the processing of more than three times the total blood volume (TBV) in a single session for PB apheresis; however, the efficiency and safety of LVL for manufacturing of tisagenlecleucel (tisa\u2010cel) remain unclear. This study aimed to investigate the tolerability of LVL.<\/jats:p><\/jats:sec><jats:sec><jats:title>Study Design and Methods<\/jats:title><jats:p>We retrospectively collected data on LVL (\u22653\u2010fold TBV) and NVL (&lt;3\u2010fold TBV) performed for patients with r\/r B\u2010ALL\/B\u2010NHL in our institution during November 2019 and September 2023. All procedures were performed using a continuous mononuclear cell collection (cMNC) protocol with the Spectra Optia.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Although pre\u2010apheresis CD3<jats:sup>+<\/jats:sup> cells in the PB were significantly lower in LVL procedures (900 vs. 348\/\u03bcL, <jats:italic>p<\/jats:italic>\u2009&lt;\u2009.01), all patients could obtain sufficient CD3<jats:sup>+<\/jats:sup> cell yield in a single day with a comparably successful rate of final products (including out\u2010of\u2010specification) between the two groups (97.2% vs. 100.0%, <jats:italic>p<\/jats:italic>\u2009=\u20091.00). The incidence and severity of citrate toxicity (no patients with grade \u2265 3) during procedures was not significantly different between the two groups (22.2% vs. 26.1%, <jats:italic>p<\/jats:italic>\u2009=\u2009.43) and no patient discontinued leukapheresis due to any complications.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>LVL procedures using Spectra Optia cMNC protocol was well tolerated and did not affect the manufacturing of tisa\u2010cel.<\/jats:p><\/jats:sec>","DOI":"10.1111\/trf.17765","type":"journal-article","created":{"date-parts":[[2024,2,29]],"date-time":"2024-02-29T07:34:28Z","timestamp":1709192068000},"page":"674-684","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Collection efficiency and safety of large\u2010volume leukapheresis for the manufacturing of 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Hematology and Oncology Okayama University Hospital  Okayama Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7322-5460","authenticated-orcid":false,"given":"Noboru","family":"Asada","sequence":"additional","affiliation":[{"name":"Department of Hematology and Oncology Okayama University Hospital  Okayama Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daisuke","family":"Ennishi","sequence":"additional","affiliation":[{"name":"Department of Hematology and Oncology Okayama University Hospital  Okayama Japan"},{"name":"Center for Comprehensive Genomic Medicine Okayama University Hospital  Okayama Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ken\u2010ichi","family":"Matsuoka","sequence":"additional","affiliation":[{"name":"Department of Hematology and Oncology Okayama University Hospital  Okayama 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