{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,11]],"date-time":"2026-02-11T02:28:15Z","timestamp":1770776895810,"version":"3.50.0"},"reference-count":30,"publisher":"Wiley","issue":"S2","license":[{"start":{"date-parts":[[2025,3,16]],"date-time":"2025-03-16T00:00:00Z","timestamp":1742083200000},"content-version":"am","delay-in-days":365,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#am"},{"start":{"date-parts":[[2024,3,16]],"date-time":"2024-03-16T00:00:00Z","timestamp":1710547200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100013132","name":"Combat Casualty Care Research Program","doi-asserted-by":"publisher","award":["B_026_2020"],"award-info":[{"award-number":["B_026_2020"]}],"id":[{"id":"10.13039\/100013132","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Transfusion"],"published-print":{"date-parts":[[2024,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Platelet concentrates (PCs) used for transfusion can be produced by apheresis or derived from whole blood (WB). The Reveos device is the first US Food and Drug Administration\u2010approved automated blood processing system that can produce PCs. In this work, we evaluated the quality and function of Reveos\u2010collected PCs stored for 7\u2009days at room temperature.<\/jats:p><\/jats:sec><jats:sec><jats:title>Study Design and Methods<\/jats:title><jats:p>WB was collected from healthy donors and componentized on the day of collection (Fresh) or after an overnight hold (Overnight). PCs were produced (<jats:italic>n<\/jats:italic>\u2009=\u20097 Fresh; <jats:italic>n<\/jats:italic>\u2009=\u20096 Overnight), stored at room temperature in plasma, and evaluated on days 1 and 7 for quality metrics, platelet activation, clot formation, and aggregation response.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Platelet count was comparable between Fresh and Overnight PCs. A drop in pH was reported in Fresh day 7 PCs (<jats:italic>p<\/jats:italic>\u2009&lt;\u2009.001, vs. day 1) but not in Overnight. Overnight units displayed the lowest levels of P\u2010selectin expression (<jats:italic>p<\/jats:italic>\u2009=\u2009.0008, vs. day 7 Fresh). Reduced clot strength and increased lysis were observed in both Fresh and Overnight units on day 7 (vs. day 1). Overnight\u2010hold PCs resulted in the highest clot strength on day 7 (<jats:italic>p<\/jats:italic>\u2009=\u2009.0084, vs. Fresh). No differences in aggregation were reported between groups.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Reveos\u2010processed PCs produced from overnight\u2010hold WB performed better in hemostatic function assays and displayed reduced activation compared to fresh WB\u2010derived PCs, although both PC groups maintained platelet quality throughout storage. Utilization of overnight WB for PC preparation with Reveos holds promise as an alternative method of producing platelets for transfusion purposes.<\/jats:p><\/jats:sec>","DOI":"10.1111\/trf.17780","type":"journal-article","created":{"date-parts":[[2024,3,16]],"date-time":"2024-03-16T11:19:34Z","timestamp":1710587974000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Evaluation of platelets componentized with the Reveos Automated Blood Processing System and stored for 7 days at room temperature in a non\u2010Di(2\u2010ethylhexyl) phthalate (DEHP) platelet pooling set"],"prefix":"10.1111","volume":"64","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2626-9780","authenticated-orcid":false,"given":"Kristin M.","family":"Reddoch\u2010Cardenas","sequence":"first","affiliation":[{"name":"Blood and Shock Resuscitation U.S. Army Institute of Surgical Research, JBSA\u2010FT  Sam Houston Texas USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Samantha L.","family":"Perez","sequence":"additional","affiliation":[{"name":"Blood and Shock Resuscitation U.S. Army Institute of Surgical Research, JBSA\u2010FT  Sam Houston Texas USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Justin N.","family":"Ferdin","sequence":"additional","affiliation":[{"name":"Blood and Shock Resuscitation U.S. Army Institute of Surgical Research, JBSA\u2010FT  Sam Houston Texas USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael A.","family":"Meledeo","sequence":"additional","affiliation":[{"name":"Blood and Shock Resuscitation U.S. Army Institute of Surgical Research, JBSA\u2010FT  Sam Houston Texas USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,3,16]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.17348"},{"key":"e_1_2_9_3_1","volume-title":"Technical manual methods and appendices","author":"AABB","year":"2020"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2010.02973.x"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1537-2995.1991.31791368338.x"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/0140-6736(92)92296-R"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1537-2995.1997.37397240204.x"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2010.02959.x"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/vox.12472"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1423-0410.1985.tb00162.x"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1537-2995.1984.24685066808.x"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00204-003-0466-7"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/vox.13384"},{"key":"e_1_2_9_14_1","unstructured":"European Commission Directorate\u2010General for Internal Market Industry Entrepreneurship and SMEs. 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