{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,25]],"date-time":"2026-10-25T05:46:58Z","timestamp":1792907218691,"version":"4.5.0"},"reference-count":48,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2024,4,9]],"date-time":"2024-04-09T00:00:00Z","timestamp":1712620800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004489","name":"Mitacs","doi-asserted-by":"publisher","award":["40000"],"award-info":[{"award-number":["40000"]}],"id":[{"id":"10.13039\/501100004489","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005242","name":"Universit\u00e9 de Montr\u00e9al","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100005242","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Transfusion"],"published-print":{"date-parts":[[2024,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>A life\u2010threatening anaphylactic shock can occur if a patient with undiagnosed immunoglobulin A (IgA) deficiency (i.e., IgA levels &lt;500\u2009ng\/mL) receives IgA\u2010containing blood, hence the need for a rapid, point\u2010of\u2010care (POC) method for IgA deficiency screening. Enzyme\u2010linked immunosorbent assay (ELISA) is routinely used to detect IgA, but this method requires trained specialists and \u226524\u2009h to obtain a result. We developed a surface plasmon resonance (SPR)\u2010based protocol to identify IgA\u2010deficient patients or donors within 1\u2009h.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and Methods<\/jats:title><jats:p>The SPR sensor relies on the detection of IgAs captured by primary antibodies adsorbed on the SPR chip and quantified with secondary antibodies. The sensor was calibrated from 0 to 2000\u2009ng\/mL in buffer, IgA\u2010depleted human serum, and plasma samples from IgA\u2010deficient individuals. A critical concentration of 500\u2009ng\/mL was set for IgA deficiency. The optimized sensor was then tested on eight plasma samples with known IgA status (determined by ELISA), including five with IgA deficiency and three with normal IgA levels.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The limit of detection was estimated at 30\u2009ng\/mL in buffer and 400\u2009ng\/mL in diluted plasma. The results obtained fully agreed with ELISA among the eight plasma samples tested. The protocol distinguished IgA\u2010deficient from normal samples, even for samples with an IgA concentration closer to critical concentration.<\/jats:p><\/jats:sec><jats:sec><jats:title>Discussion<\/jats:title><jats:p>In conclusion, we developed a reliable POC assay for the quantification of IgA in plasma. This test may permit POC testing at blood drives and centralized centers to maintain reserves of IgA\u2010deficient blood and in\u2010hospital testing of blood recipients.<\/jats:p><\/jats:sec>","DOI":"10.1111\/trf.17818","type":"journal-article","created":{"date-parts":[[2024,4,9]],"date-time":"2024-04-09T08:17:52Z","timestamp":1712650672000},"page":"881-892","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["A portable surface plasmon resonance (<scp>SPR<\/scp>) sensor for the detection of immunoglobulin <scp>A<\/scp> in plasma"],"prefix":"10.1111","volume":"64","author":[{"ORCID":"https:\/\/orcid.org\/0009-0003-8195-4438","authenticated-orcid":false,"given":"Caroline","family":"Dubois","sequence":"first","affiliation":[{"name":"D\u00e9partement de Chimie, Quebec Center for Advanced Materials, Regroupement Qu\u00e9b\u00e9cois sur les Mat\u00e9riaux de Pointe, and Centre Interdisciplinaire de Recherche sur le 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