{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T10:46:25Z","timestamp":1788345985704,"version":"build-2803163510"},"reference-count":25,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2024,5,1]],"date-time":"2024-05-01T00:00:00Z","timestamp":1714521600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000050","name":"National Heart, Lung, and Blood Institute","doi-asserted-by":"publisher","award":["K23HL146901"],"award-info":[{"award-number":["K23HL146901"]}],"id":[{"id":"10.13039\/100000050","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Transfusion"],"published-print":{"date-parts":[[2024,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Post\u2010transfusion survival of donor red blood cells (RBCs) is important for effective chronic transfusion therapy in conditions including sickle cell disease (SCD). Biotin labeling RBCs allows direct in vivo measurement of multiple donor RBC units simultaneously post\u2010transfusion.<\/jats:p><\/jats:sec><jats:sec><jats:title>Study Design and Methods<\/jats:title><jats:p>In an observational trial of patients with SCD receiving monthly chronic transfusion therapy, aliquots of RBCs from one transfusion episode were biotin\u2010labeled and infused along with the unlabeled RBC units. Serial blood samples were obtained to measure RBC survival. Donor units were tested for RBC indices, hemoglobin fractionation, and glucose\u20106\u2010phosphate dehydrogenase (G6PD) enzyme activity. For microcytic donor RBCs (MCV\u2009&lt;\u200970\u2009fL), <jats:italic>HBA1<\/jats:italic> and <jats:italic>HBA2<\/jats:italic> genetic testing was performed on whole blood.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>We present one recipient, a pediatric patient with SCD and splenectomy who received two RBC units with aliquots from each unit labeled at distinct biotin densities (2 and 18\u2009\u03bcg\/mL biotin). One donor unit was identified to have microcytosis (MCV 68.5\u2009fL after biotinylation); whole blood sample obtained at a subsequent donation showed 2\u2010gene deletion alpha\u2010thalassemia trait (\u0251\u2013<jats:sup>3.7kb<\/jats:sup>\/\u0251\u2013<jats:sup>3.7kb<\/jats:sup>) and normal serum ferritin. G6PD activity was &gt;60% of normal mean for both. The RBCs with alpha\u2010thalassemia RBC had accelerated clearance and increased surface phosphatidylserine post\u2010transfusion, as compared with the normocytic RBC (half life 65 vs. 86\u2009days, respectively).<\/jats:p><\/jats:sec><jats:sec><jats:title>Discussion<\/jats:title><jats:p>Post\u2010transfusion RBC survival may be lower for units from donors with alpha\u2010thalassemia trait, although the impact of thalassemia trait donors on transfusion efficacy requires further study.<\/jats:p><\/jats:sec>","DOI":"10.1111\/trf.17857","type":"journal-article","created":{"date-parts":[[2024,5,2]],"date-time":"2024-05-02T02:21:40Z","timestamp":1714616500000},"page":"1109-1115","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Survival of transfused red blood cells from a donor with alpha\u2010thalassemia trait in a recipient with sickle cell disease"],"prefix":"10.1111","volume":"64","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6082-8385","authenticated-orcid":false,"given":"Marianne E. M.","family":"Yee","sequence":"first","affiliation":[{"name":"Aflac Cancer and Blood Disorders Center Children's Healthcare of Atlanta  Atlanta Georgia USA"},{"name":"Department of Pediatrics Emory University School of Medicine  Atlanta Georgia USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5452-4529","authenticated-orcid":false,"given":"Mischa L.","family":"Covington","sequence":"additional","affiliation":[{"name":"Joint Program in Transfusion Medicine Brigham and Women's Hospital, Harvard Medical School  Boston Massachusetts USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patricia E.","family":"Zerra","sequence":"additional","affiliation":[{"name":"Aflac Cancer and Blood Disorders Center Children's Healthcare of Atlanta  Atlanta Georgia USA"},{"name":"Department of Pediatrics Emory University School of Medicine  Atlanta Georgia USA"},{"name":"Center for Transfusion and Cellular Therapies, Department of Pathology and Laboratory Medicine Emory University School of Medicine  Atlanta Georgia USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James W.","family":"McCoy","sequence":"additional","affiliation":[{"name":"Center for Transfusion and Cellular Therapies, Department of Pathology and Laboratory Medicine Emory University School of Medicine  Atlanta Georgia USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kirk A.","family":"Easley","sequence":"additional","affiliation":[{"name":"Department of Biostatistics and Bioinformatics Rollins School of Public Health  Atlanta Georgia USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Clinton H.","family":"Joiner","sequence":"additional","affiliation":[{"name":"Aflac Cancer and Blood Disorders Center Children's Healthcare of Atlanta  Atlanta Georgia USA"},{"name":"Department of Pediatrics Emory University School of Medicine  Atlanta Georgia USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Janetta","family":"Bryksin","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine Emory University School of Medicine  Atlanta Georgia USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard O.","family":"Francis","sequence":"additional","affiliation":[{"name":"Department of Pathology and Cell Biology Columbia University Medical Center, New York Presbyterian Hospital  New York New York USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christopher M.","family":"Lough","sequence":"additional","affiliation":[{"name":"LifeSouth Community Blood Centers  Gainesville Florida USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-9117-0438","authenticated-orcid":false,"given":"Niren","family":"Patel","sequence":"additional","affiliation":[{"name":"Department of Medicine Medical College of Georgia at Augusta University  Augusta Georgia USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abdullah","family":"Kutlar","sequence":"additional","affiliation":[{"name":"Department of Medicine Medical College of Georgia at Augusta University  Augusta Georgia USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cassandra D.","family":"Josephson","sequence":"additional","affiliation":[{"name":"Cancer and Blood Disorders Institute Johns Hopkins All Children's Hospital  St. Petersburg Florida USA"},{"name":"Departments of Oncology and Pediatrics Johns Hopkins University School of Medicine  Baltimore Maryland USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John D.","family":"Roback","sequence":"additional","affiliation":[{"name":"Center for Transfusion and Cellular Therapies, Department of Pathology and Laboratory Medicine Emory University School of Medicine  Atlanta Georgia USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1130-9551","authenticated-orcid":false,"given":"Sean R.","family":"Stowell","sequence":"additional","affiliation":[{"name":"Joint Program in Transfusion Medicine Brigham and Women's Hospital, Harvard Medical School  Boston Massachusetts USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8692-4041","authenticated-orcid":false,"given":"Ross M.","family":"Fasano","sequence":"additional","affiliation":[{"name":"Aflac Cancer and Blood Disorders Center Children's Healthcare of Atlanta  Atlanta Georgia USA"},{"name":"Center for Transfusion and Cellular Therapies, Department of Pathology and Laboratory Medicine Emory University School of Medicine  Atlanta Georgia 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