{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T22:18:29Z","timestamp":1790806709642,"version":"4.1.0"},"reference-count":24,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2024,6,15]],"date-time":"2024-06-15T00:00:00Z","timestamp":1718409600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Transfusion"],"published-print":{"date-parts":[[2024,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>The large dengue (DENV) and chikungunya (CHIKV) outbreaks observed during the last decade across the world, as well as local transmissions in non\u2010endemic areas are a growing concern for blood safety. The aim of this study was to evaluate and compare the sensitivity of nucleic acid tests (NAT) detecting DENV and CHIKV RNA.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and Methods<\/jats:title><jats:p>Using DENV 1 to 4 International Standards, the limits of detection (LODs) calculated by probit analysis of two NAT assays; the cobas CHIKV\/DENV assay (Roche Diagnostics) and the Procleix Dengue Virus Assay (Grifols) were compared. In addition, CHIKV\u2010RNA LOD of the cobas CHIKV\/DENV assay was evaluated.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>For dengue, the 95% LOD of the cobas assay ranged between 4.10 [CI95%: 2.70\u20138.19] IU\/mL (DENV\u20102) and 7.07 [CI95%: 4.34\u201314.89] IU\/mL (DENV\u20104), and between 2.19 [CI95%: 1.53\u20133.83] IU\/mL (DENV\u20103) and 5.84 [CI95%: 3.84\u201310.77] IU\/mL (DENV\u20101) for Procleix assay. The Procleix assay had a significant lower LOD for DENV\u20103 (2.19 vs. 5.89\u2009IU\/mL) when compared to the cobas assay (<jats:italic>p<\/jats:italic>\u2009=\u20090.005). The 95% LOD for CHIKV\u2010RNA detection of the cobas assay was 4.76 [CI95%: 3.08\u20138.94] IU\/mL.<\/jats:p><\/jats:sec><jats:sec><jats:title>Discussion<\/jats:title><jats:p>The two NAT assays developed for blood donor screening evaluated in this study demonstrated high and similar analytical performance. Subject to an appropriate risk\u2010benefit assessment, they can be used to support blood safety during outbreaks in endemic areas or in non\u2010endemic areas as an alternative to deferring blood donors during local transmission likely to affect the blood supply. The development of multiplex assays is expected to optimize laboratory organization.<\/jats:p><\/jats:sec>","DOI":"10.1111\/trf.17921","type":"journal-article","created":{"date-parts":[[2024,6,15]],"date-time":"2024-06-15T09:33:20Z","timestamp":1718444000000},"page":"1503-1508","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Evaluation of assays for nucleic acid testing for the prevention of chikungunya and dengue virus transmission by blood transfusion"],"prefix":"10.1111","volume":"64","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3310-5808","authenticated-orcid":false,"given":"Pierre","family":"Gallian","sequence":"first","affiliation":[{"name":"Etablissement Fran\u00e7ais du Sang  La Plaine Saint Denis France"},{"name":"Unit\u00e9 des Virus Emergents (UVE: Aix\u2010Marseille Univ\u2014IRD 190\u2014Inserm 1207)  Marseille France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isabelle","family":"Dupont","sequence":"additional","affiliation":[{"name":"Etablissement Fran\u00e7ais du Sang Bretagne  Brest France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marjorie","family":"Lacoste","sequence":"additional","affiliation":[{"name":"Etablissement Fran\u00e7ais du Sang Occitanie  Toulouse France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nad\u00e8ge","family":"Brisbarre","sequence":"additional","affiliation":[{"name":"Unit\u00e9 des Virus Emergents (UVE: Aix\u2010Marseille Univ\u2014IRD 190\u2014Inserm 1207)  Marseille France"},{"name":"Etablissement Fran\u00e7ais du Sang Provence Alpes C\u00f4te d'Azur et Corse  Marseille France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christine","family":"Isnard","sequence":"additional","affiliation":[{"name":"Unit\u00e9 des Virus Emergents (UVE: Aix\u2010Marseille Univ\u2014IRD 190\u2014Inserm 1207)  Marseille France"},{"name":"Etablissement Fran\u00e7ais du Sang Provence Alpes C\u00f4te d'Azur et Corse  Marseille France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Idriss","family":"Delouane","sequence":"additional","affiliation":[{"name":"\u00c9tablissement fran\u00e7ais du sang, La R\u00e9union\u2014Oc\u00e9an Indien. 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