{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,10]],"date-time":"2026-05-10T10:22:43Z","timestamp":1778408563712,"version":"3.51.4"},"reference-count":31,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2024,7,23]],"date-time":"2024-07-23T00:00:00Z","timestamp":1721692800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Transfusion"],"published-print":{"date-parts":[[2024,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Transfusion\u2010related acute lung injury caused by antibodies against human neutrophil antigens (HNA) is a serious but rare complication associated with blood transfusion. The presence of such antibodies is most probable in donors with a transfusion\/pregnancy history. During the COVID\u201019 pandemic period convalescent plasma (CP) containing neutralizing antibodies against SARS\u2010CoV\u20102 was widely used for COVID\u201019 patients as a therapy in the absence of any treatment. The aim of the study was to work out a simple diagnostic algorithm of anti\u2010HNA testing of allo\u2010exposed CP donors including genetic HNA screening.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and Methods<\/jats:title><jats:p>A total of 457 anti\u2010HLA\u2010negative allo\u2010exposed CP donors were genotyped for HNA\u20101a\/1b, HNA\u20103a\/3b, and HNA\u20102, and only donors with homozygous HNA\u20101a\/1a; HNA\u20103b\/3b; or HNA\u20102null genotypes were tested for anti\u2010HNA antibody using LabScreenMulti (One Lambda) and homozygous HNA\u20101b\/1b using the granulocyte immunofluorescence test (GIFT) but verified using LabScreenMulti.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Testing of 83 homozygous HNA\u20103b\/3b; HNA\u20102null; or HNA\u20101a\/1a donors revealed anti\u2010HNA\u20103a antibody in one case. Testing of 181 HNA\u20101b\/1b donors using GIFT gave 10 ambiguous results verified using LabScreenMulti which confirmed anti\u2010HNA\u20101a antibody in one case. The frequency of <jats:italic>FCGR3B*01<\/jats:italic> and <jats:italic>*04<\/jats:italic> encoding HNA\u20101a was 0.34; <jats:italic>FCGR3B*02<\/jats:italic>, <jats:italic>*03<\/jats:italic>, and <jats:italic>*05<\/jats:italic> encoding HNA\u20101b\u20140.66; <jats:italic>SLC44A2*01<\/jats:italic> encoding HNA\u20103a\u20140.80; and <jats:italic>SLC44A2*02<\/jats:italic> encoding HNA\u20103b\u20140.20. In 3.7% cases the HNA\u20102null genotype was revealed.<\/jats:p><\/jats:sec><jats:sec><jats:title>Discussion<\/jats:title><jats:p>Due to applying HNA genotyping as a primary test before anti\u2010HNA antibody testing the serological work was limited only to HNA\u2010homozygous donors revealing two anti\u2010HNA immunized donors. The distribution of HNA genotypes in the cohort was similar to other Caucasian populations.<\/jats:p><\/jats:sec>","DOI":"10.1111\/trf.17962","type":"journal-article","created":{"date-parts":[[2024,7,24]],"date-time":"2024-07-24T08:10:10Z","timestamp":1721808610000},"page":"1703-1708","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Anti\u2010<scp>HNA<\/scp> testing of allo\u2010exposed <scp>COVID<\/scp>\u201019 convalescent plasma donors including genetic <scp>human neutrophil antigen<\/scp> screening to prevent anti\u2010<scp>HNA<\/scp> antibody\u2010mediated <scp>transfusion\u2010related acute lung injury<\/scp>"],"prefix":"10.1111","volume":"64","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2250-2226","authenticated-orcid":false,"given":"Katarzyna","family":"Guz","sequence":"first","affiliation":[{"name":"Department of Hematological and Transfusion Immunology Institute of Hematology and Transfusion Medicine 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Transfusion Immunology Institute of Hematology and Transfusion Medicine  Warsaw Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Magdalena","family":"Krzemienowska","sequence":"additional","affiliation":[{"name":"Department of Hematological and Transfusion Immunology Institute of Hematology and Transfusion Medicine  Warsaw Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sylwia","family":"Purchla\u2010Szepio\u0142a","sequence":"additional","affiliation":[{"name":"Department of Hematological and Transfusion Immunology Institute of Hematology and Transfusion Medicine  Warsaw Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anna","family":"G\u0142\u00f3wka","sequence":"additional","affiliation":[{"name":"Department of Hematological and Transfusion Immunology Institute of Hematology and Transfusion Medicine  Warsaw 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