{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,5]],"date-time":"2026-01-05T07:33:34Z","timestamp":1767598414125,"version":"3.40.5"},"reference-count":44,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2024,9,20]],"date-time":"2024-09-20T00:00:00Z","timestamp":1726790400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003196","name":"Ministero della Salute","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003196","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Transfusion"],"published-print":{"date-parts":[[2024,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Thrombocytopenia, common in preterm newborns, may increase bleeding risk and is often treated with transfusions. Recent studies reveal that transfusing platelets at a high threshold worsens outcomes, possibly due to a \u201cdevelopmental mismatch\u201d between adult\u2010derived platelets and neonatal hemostatic system. Cord blood\u2010derived platelet concentrates (CBPCs) could be an alternative for newborns. Our study aims to produce and evaluate the quality parameters of CBPCs during storage.<\/jats:p><\/jats:sec><jats:sec><jats:title>Study Design and Methods<\/jats:title><jats:p>Cord blood was collected from placentas after near\u2010term and full\u2010term pregnancies. Several production methods were attempted to obtain CBPCs, varying centrifugation settings, filtration, and dilution procedures. Adult\u2010derived platelet concentrates (PCs) processed with the same methods, and standard PCs from five buffy\u2010coats were used as controls. Storage tests were performed on days 2, 4\u20135, 7 from the collection.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>CBPCs parameters were compared with adult\u2010derived PCs, and no significant differences were found for mean platelet volume (MPV), swirling, morphology, glucose, lactate, pCO<jats:sub>2<\/jats:sub>, and pO<jats:sub>2<\/jats:sub>. pH and bicarbonate were lower in CBPCs. Some significant differences between methods in CD62P expression and JC\u20101 ratio were observed. Compared with standard PCs, CBPCs showed lower platelet concentration, pH, and JC\u20101. Additionally, both in CBPCs as well as in control PCs, the apoptosis marker phosphatidylserine was elevated.<\/jats:p><\/jats:sec><jats:sec><jats:title>Discussion<\/jats:title><jats:p>CBPCs were of comparable quality to control PCs during storage. However, apoptosis markers in both groups were elevated, suggesting processing and storage of low volumes of PCs require further optimization. Also, filtration of low volumes leads to significant platelet loss, an issue that requires remedy.<\/jats:p><\/jats:sec>","DOI":"10.1111\/trf.18007","type":"journal-article","created":{"date-parts":[[2024,9,21]],"date-time":"2024-09-21T06:44:15Z","timestamp":1726901055000},"page":"1968-1979","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Storage properties of platelet concentrates from umbilical cord blood prepared using three different methods"],"prefix":"10.1111","volume":"64","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2083-411X","authenticated-orcid":false,"given":"Valeria","family":"Cortesi","sequence":"first","affiliation":[{"name":"Neonatal Intensive Care Unit Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico  Milan Italy"},{"name":"Department of Clinical Sciences and Community Health Universit\u00e0 Degli Studi di Milano  Milan Italy"},{"name":"Division of Neonatology, Department of Pediatrics Leiden University Medical Center  Leiden The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Enrico","family":"Lopriore","sequence":"additional","affiliation":[{"name":"Division of Neonatology, Department of Pediatrics Leiden University Medical Center  Leiden The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4921-1437","authenticated-orcid":false,"given":"Giacomo","family":"Cavallaro","sequence":"additional","affiliation":[{"name":"Neonatal Intensive Care Unit Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico  Milan Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9103-7148","authenticated-orcid":false,"given":"Ido J.","family":"Bontekoe","sequence":"additional","affiliation":[{"name":"Department of Product and Process Development Sanquin Blood Bank  Amsterdam The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pieter F.","family":"van der Meer","sequence":"additional","affiliation":[{"name":"Department of Product and Process Development Sanquin Blood Bank  Amsterdam The Netherlands"},{"name":"Department of Hematology Haga Teaching Hospital  The Hague The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Susanna","family":"Fustolo\u2010Gunnink","sequence":"additional","affiliation":[{"name":"Division of Neonatology, Department of Pediatrics Leiden University Medical Center  Leiden The Netherlands"},{"name":"Sanquin Blood Supply Foundation  Amsterdam The Netherlands"},{"name":"Department of Pediatric Hematology, Emma Children's Hospital Amsterdam University Medical Center (UMC), University of Amsterdam  Amsterdam The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Genny","family":"Raffaeli","sequence":"additional","affiliation":[{"name":"Neonatal Intensive Care Unit Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico  Milan Italy"},{"name":"Department of Clinical Sciences and Community Health Universit\u00e0 Degli Studi di Milano  Milan Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0621-155X","authenticated-orcid":false,"given":"Stefano","family":"Ghirardello","sequence":"additional","affiliation":[{"name":"Neonatal Intensive Care Unit Fondazione IRCCS Policlinico S. 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