{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T14:58:38Z","timestamp":1790089118216,"version":"4.0.1"},"reference-count":14,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2024,12,8]],"date-time":"2024-12-08T00:00:00Z","timestamp":1733616000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000014","name":"Canadian Blood Services","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000014","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Transfusion"],"published-print":{"date-parts":[[2025,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>The ACP 215 automated cell processor is used to glycerolize and deglycerolize red cell concentrates (RCCs). Its primary advantage over the COBE 2991, previously used to cryopreserve RCCs, is that it maintains a closed system enabling extended post\u2010thaw expiry. However, it was observed that post\u2010deglycerolization hematocrits (Hct) of units processed with the LN236 kit are markedly lower than those processed using the COBE 2991. Therefore, we intended to determine whether a modified process using a smaller volume deglycerolization kit (LN235) could increase the final Hct with limited deleterious effects on product characteristics.<\/jats:p><\/jats:sec><jats:sec><jats:title>Study Design and Methods<\/jats:title><jats:p>Two proof\u2010of\u2010concept (POC) studies, conducted to determine the feasibility of using the LN235 processing kit for deglycerolization, identified the necessary modifications to the pre\u2010 and post\u2010deglycerolization process, after which a two\u2010part study characterized the modified protocol. The impact of pre\u2010cryopreservation storage duration (7\u201321\u2009days), input red cell mass, and the type of CPD\/SAGM RCC production method (red cell filtration and whole blood filtration) were investigated.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Using the LN235 kit in conjunction with a volume reduction step for RCCs with a red cell mass exceeding 180\u2009mL allowed for an ~8% increase in Hct. As expected, slightly lower recoveries were seen for large RCCs due to volume reduction; however, there were no other detrimental outcomes on product quality.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Leveraging the LN235 kit, recommended by Haemonetics for units with a red cell mass of \u2264180\u2009mL, can be used to increase the post\u2010deglycerolization Hct of RCCs that exceed this volume.<\/jats:p><\/jats:sec>","DOI":"10.1111\/trf.18088","type":"journal-article","created":{"date-parts":[[2024,12,9]],"date-time":"2024-12-09T04:09:52Z","timestamp":1733717392000},"page":"194-201","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Impact of input volume on red cell quality in deglycerolized <scp>RBCs<\/scp> using a modified <scp>ACP<\/scp>\u2010215 protocol"],"prefix":"10.1111","volume":"65","author":[{"given":"Anita","family":"Howell","sequence":"first","affiliation":[{"name":"Innovation and Portfolio Management Canadian Blood Services  Edmonton Alberta Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Angela","family":"Hill","sequence":"additional","affiliation":[{"name":"Innovation and Portfolio Management Canadian Blood Services  Edmonton Alberta Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wanda","family":"Lefresne","sequence":"additional","affiliation":[{"name":"Supply Chain Process Management Canadian Blood Services  Dartmouth Nova Scotia Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brandie","family":"Dennis","sequence":"additional","affiliation":[{"name":"Supply Chain Process Management Canadian Blood Services  Edmonton Alberta Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tracey 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P.","family":"Acker","sequence":"additional","affiliation":[{"name":"Innovation and Portfolio Management Canadian Blood Services  Edmonton Alberta Canada"},{"name":"Department of Laboratory Medicine and Pathology University of Alberta  Edmonton Alberta Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,12,8]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/vox.12218"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.0958-7578.2004.00472.x"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2005.00588.x"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mjafi.2013.06.005"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.21307\/immunohematology-2019-152"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.17138"},{"key":"e_1_2_8_8_1","first-page":"189","article-title":"The preparation of frozen red blood cells and a procedure for deglycerolizing frozen RBCs using COBE 2991 blood cell processor","volume":"12","author":"Choi KH","year":"2001","journal-title":"Korean J Blood Transfus"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1159\/000449039"},{"key":"e_1_2_8_10_1","first-page":"166","volume-title":"Transfusion\u2010associated circulatory overload","author":"Popovsky MA","year":"2008"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/vox.12836"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.12679"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.3233\/CH-2010-1384"},{"key":"e_1_2_8_14_1","volume-title":"Z902:20 blood and blood components","author":"Canadian Standards 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