{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T15:05:44Z","timestamp":1789657544442,"version":"4.0.1"},"reference-count":40,"publisher":"Wiley","issue":"S1","license":[{"start":{"date-parts":[[2025,2,5]],"date-time":"2025-02-05T00:00:00Z","timestamp":1738713600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2025,2,5]],"date-time":"2025-02-05T00:00:00Z","timestamp":1738713600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Transfusion"],"published-print":{"date-parts":[[2025,5]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Non\u2010refrigerated whole blood can be used for bleeding emergencies when cold\u2010stored whole blood is unavailable. Storage time in room temperature is usually limited to 24\u2009h although there is little evidence supporting this practice. We studied the quality of whole blood stored in room temperature for 5\u2009days to investigate the effects of prolonged storage time.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Study Design and Methods<\/jats:title>\n                    <jats:p>Non\u2010leukoreduced whole blood in CPDA\u20101 from 10 group O or A RhD positive male donors was stored in +22\u00b0C for 120\u2009h. Samples were taken daily to assess blood cultures, blood count and metabolic parameters. Platelet function and blood coagulation were evaluated with multiple electrode aggregometry, viscoelastic tests (sonorheometry and rotational thromboelastometry), thrombin generation assay and measurements of individual clotting factors.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Blood cell counts remained stable during storage. Metabolic changes were similar to those previously reported in cold\u2010stored blood products. Most coagulation factor levels, including FVIII, decreased during storage but remained within physiological range. Thrombin generation remained mostly intact during storage. In viscoelastic tests, clotting times prolonged, but clot strength remained stable. Platelet function in multiple electrode aggregometry impaired along with storage. No bacterial growth was detected in any sample.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Whole blood stored in room temperature for 5\u2009days seems bacteriologically safe and retains most of its metabolic and hemostatic function. These results suggest that whole blood stored in room temperature may be usable for longer than the currently recommended 24\u2009h.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/trf.18133","type":"journal-article","created":{"date-parts":[[2025,2,5]],"date-time":"2025-02-05T13:25:16Z","timestamp":1738761916000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Quality of whole blood stored in room temperature for up to 5\u2009days"],"prefix":"10.1111","volume":"65","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5131-0656","authenticated-orcid":false,"given":"Sanna","family":"Susila","sequence":"first","affiliation":[{"name":"Finnish Red Cross Blood Service  Vantaa Finland"},{"name":"Department of Anesthesiology and Intensive Care Medicine Helsinki University Hospital and University of Helsinki  Helsinki Finland"},{"name":"Emergency Medical Service and Emergency Department P\u00e4ij\u00e4t\u2010H\u00e4me Wellbeing Services County  Lahti Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5273-8088","authenticated-orcid":false,"given":"Tuukka","family":"Helin","sequence":"additional","affiliation":[{"name":"Department of Clinical Chemistry HUS Diagnostic Center, Helsinki University Hospital and University of Helsinki  Helsinki Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7355-2354","authenticated-orcid":false,"given":"Lotta","family":"Joutsi\u2010Korhonen","sequence":"additional","affiliation":[{"name":"Department of Clinical Chemistry HUS Diagnostic Center, Helsinki University Hospital and University of Helsinki  Helsinki Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jouni","family":"Lauronen","sequence":"additional","affiliation":[{"name":"Finnish Red Cross Blood Service  Vantaa Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8469-4091","authenticated-orcid":false,"given":"Minna","family":"Ilmakunnas","sequence":"additional","affiliation":[{"name":"Finnish Red Cross Blood Service  Vantaa Finland"},{"name":"Department of Anesthesiology and Intensive Care Medicine Helsinki University Hospital and University of Helsinki  Helsinki Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,2,5]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.surg.2021.05.051"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.16968"},{"key":"e_1_2_8_4_1","unstructured":"Committee on Tactical Combat Casualty Care.Tactical Combat Casualty Care (TCCC) Guidelines [Internet]. 2024 [cited 29.12.2024]. 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