{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T14:58:38Z","timestamp":1790089118159,"version":"4.0.1"},"reference-count":19,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2025,2,10]],"date-time":"2025-02-10T00:00:00Z","timestamp":1739145600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000014","name":"Canadian Blood Services","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000014","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Transfusion"],"published-print":{"date-parts":[[2025,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Historically, red cell concentrates (RCCs) have been manually glycerolized and deglycerolized using an open system (COBE 2991, Terumo). Implementation of a closed system cell processor (ACP\u2010215, Haemonetics) for glycerolization and deglycerolization of RCCs creates a challenge for management of the historic cryopreserved RCC inventory. A study was undertaken to determine whether manually glycerolized frozen RCCs could be deglycerolized using the closed system processor, as the open system processors are being discontinued.<\/jats:p><\/jats:sec><jats:sec><jats:title>Study Design and Methods<\/jats:title><jats:p>Thirteen ABO\/Rh matched RCCs were pooled and split to produce six large (approximately 354\u2009mL) and six small (approximately 244\u2009mL) RCCs. All units were stored for 14\u2009days post\u2010collection, manually glycerolized and frozen at \u2264 \u221265\u00b0C for \u226572\u2009h. Half of the units of each size were deglycerolized using the COBE 2991 and resuspended in 0.9% saline, and the remaining units were centrifuged, deglycerolized on the ACP\u2010215, and resuspended AS\u20103. RBC quality was tested at 24\u2009\u00b1\u20092\u2009h post\u2010deglycerolization.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>All units deglycerolized on the ACP\u2010215 had significantly lower hemolysis (<jats:italic>p<\/jats:italic>\u2009&lt;\u2009.001) levels than those processed on the COBE2991. Large ACP\u2010215 deglycerolized units had lower hematocrits (<jats:italic>p<\/jats:italic>\u2009&lt;\u2009.05), hemoglobin (<jats:italic>p<\/jats:italic>\u2009&lt;\u2009.01), and recovery (<jats:italic>p<\/jats:italic>\u2009=\u2009.001) than did large units deglycerolized on the COBE 2991. All ACP\u2010215\u2009units met the regulatory standards for hemolysis, hematocrit, hemoglobin, and recovery.<\/jats:p><\/jats:sec><jats:sec><jats:title>Discussion<\/jats:title><jats:p>The closed\u2010system ACP\u2010215 processor significantly reduced post\u2010deglycerolization hemolysis in all units, and hemoglobin content in large units. The ACP\u2010215, in combination with a centrifugation step, is suitable for processing cryopreserved RCCs that have been manually glycerolized.<\/jats:p><\/jats:sec>","DOI":"10.1111\/trf.18156","type":"journal-article","created":{"date-parts":[[2025,2,11]],"date-time":"2025-02-11T05:33:49Z","timestamp":1739252029000},"page":"664-668","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Deglycerolization of manually glycerolized, frozen red cell concentrates using a closed system cell processor"],"prefix":"10.1111","volume":"65","author":[{"given":"Anita","family":"Howell","sequence":"first","affiliation":[{"name":"Innovation and Portfolio Management, Canadian Blood Services  Edmonton Alberta Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jayme","family":"Kurach","sequence":"additional","affiliation":[{"name":"Innovation and Portfolio Management, Canadian Blood Services  Edmonton Alberta 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Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,2,10]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1537-2995.2003.00293.x"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.transci.2005.11.008"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537\u20102995.1973.tb05457.x"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/0955-3886(89)90025-8"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2005.00588.x"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.17138"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0377-1237(09)80057-9"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mjafi.2013.06.005"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/vox.12218"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.21307\/immunohematology-2019-457"},{"key":"e_1_2_7_12_1","unstructured":"European 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