{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T15:05:08Z","timestamp":1789657508407,"version":"4.0.1"},"reference-count":18,"publisher":"Wiley","issue":"S1","license":[{"start":{"date-parts":[[2025,3,25]],"date-time":"2025-03-25T00:00:00Z","timestamp":1742860800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,3,25]],"date-time":"2025-03-25T00:00:00Z","timestamp":1742860800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Transfusion"],"published-print":{"date-parts":[[2025,5]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Whole blood (WB) is an efficient product for field medical resuscitation because of its unitary composition, tolerance for storage on ice and in field refrigerators, and simplicity of use. We measured quality parameters of a novel 8\u2010week WB storage system.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Study Design and Methods<\/jats:title>\n                    <jats:p>Here, 500\u2009mL of WB from healthy donors was collected in 70\u2009mL of CPDA\u20101, leukoreduced with a platelet\u2010sparing filter, pooled into ABO\u2010compatible two\u2010unit pools, and split into matched pairs of equal volume designated as Test or Control units. Test units received an additional 50\u2009mL of a novel WB preservative solution (APEX units, Hemerus Medical, St Paul, MN). A total of 15 paired WB units were evaluated at Day 0 (D0) and periodically up to Day 56 (D56) of storage at 1\u20136\u00b0C across two centers. Quality testing included cellularity, ATP concentrations, hemolysis, blood gases, metabolites, coagulation factor levels, thromboelastography (TEG), and bacterial culture.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      At D56, APEX units displayed higher RBC ATP concentration (3.14 vs. 2.18\u2009\u03bcmol\/gHb,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.001), pH (6.53 vs. 6.50,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.01), and higher bicarbonate reserve (8 vs. 5.4,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.0001). D56 APEX units had greater platelet contribution to TEG clot strength (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.01) and better preservation of red cell ATP (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.001). Activities of fibrinogen, factor VIII, factor V, and protein S activity in APEX units remained within the reference levels on D56. No bacterial contamination was detected at the end of storage.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Discussion<\/jats:title>\n                    <jats:p>These findings suggest that APEX preserves RBCs effectively and maintains platelet and plasma coagulation functions for up to 56\u2009days.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/trf.18207","type":"journal-article","created":{"date-parts":[[2025,3,28]],"date-time":"2025-03-28T05:40:37Z","timestamp":1743140437000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Novel anticoagulant\u2010preservative solution maintained the hemostatic function of cold stored whole blood for 56\u2009days"],"prefix":"10.1111","volume":"65","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2626-9780","authenticated-orcid":false,"given":"K. M.","family":"Reddoch\u2010Cardenas","sequence":"first","affiliation":[{"name":"U.S. Army Institute of Surgical Research (USAISR)  Sam Houston Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1291-7233","authenticated-orcid":false,"given":"J. A.","family":"Cancelas","sequence":"additional","affiliation":[{"name":"Hoxworth Blood Center  Cincinnati Ohio USA"},{"name":"Dana\u2010Farber Cancer Institute Harvard Medical School  Boston Massachusetts USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Nestheide","sequence":"additional","affiliation":[{"name":"Hoxworth Blood Center  Cincinnati Ohio USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N.","family":"Rugg","sequence":"additional","affiliation":[{"name":"Hoxworth Blood Center  Cincinnati Ohio USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K.","family":"Pe\u00f1a","sequence":"additional","affiliation":[{"name":"Hoxworth Blood Center  Cincinnati Ohio USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C. S.","family":"McIntosh","sequence":"additional","affiliation":[{"name":"U.S. Army Institute of Surgical Research (USAISR)  Sam Houston Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Ferdin","sequence":"additional","affiliation":[{"name":"U.S. Army Institute of Surgical Research (USAISR)  Sam Houston Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Talackine","sequence":"additional","affiliation":[{"name":"U.S. Army Institute of Surgical Research (USAISR)  Sam Houston Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Parker","sequence":"additional","affiliation":[{"name":"Hoxworth Blood Center  Cincinnati Ohio USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L. A.","family":"Jensen","sequence":"additional","affiliation":[{"name":"Hemerus Medical, LLC  Saint Paul Minnesota USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R.","family":"Gonzales","sequence":"additional","affiliation":[{"name":"Hemerus Medical, LLC  Saint Paul Minnesota USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8596-4420","authenticated-orcid":false,"given":"J. R.","family":"Hess","sequence":"additional","affiliation":[{"name":"Hemerus Medical, LLC  Saint Paul Minnesota USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Zia","sequence":"additional","affiliation":[{"name":"Hemerus Medical, LLC  Saint Paul Minnesota USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,3,25]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0708160104"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.12804"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.13253"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.16657"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/vox.13005"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.15164"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tmrv.2020.09.007"},{"key":"e_1_2_9_9_1","volume-title":"Procedure for determining packed cell volume by the microhematocrit method: approved standard","author":"Bull BS","year":"2000"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.14084"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2010.02959.x"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1423-0410.1975.tb02756.x"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.12867"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/0955-3886(94)90085-X"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2005.00672.x"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2007.01339.x"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1093\/milmed\/usae187"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.15748"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1177\/014107689909200703"}],"container-title":["Transfusion"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/trf.18207","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/trf.18207","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/trf.18207","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T14:23:27Z","timestamp":1789655007000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/trf.18207"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,3,25]]},"references-count":18,"journal-issue":{"issue":"S1","published-print":{"date-parts":[[2025,5]]}},"alternative-id":["10.1111\/trf.18207"],"URL":"https:\/\/doi.org\/10.1111\/trf.18207","archive":["Portico"],"relation":{},"ISSN":["0041-1132","1537-2995"],"issn-type":[{"value":"0041-1132","type":"print"},{"value":"1537-2995","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,3,25]]},"assertion":[{"value":"2024-12-30","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-03-01","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-03-25","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}