{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T14:58:39Z","timestamp":1790089119132,"version":"4.0.1"},"reference-count":15,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2025,3,14]],"date-time":"2025-03-14T00:00:00Z","timestamp":1741910400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Transfusion"],"published-print":{"date-parts":[[2025,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Many blood services maintain large inventories of red blood cell (RBC) units cryopreserved in glycerol using the Meryman method. With the discontinuation of the COBE\u00ae 2991 cell processor, an alternative thawing method is needed. We aimed to develop a deglycerolization protocol for Meryman\u2010frozen units using the ACP\u00ae 215 cell washer.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>In the optimization phase, Meryman\u2010frozen RBC units stored for 10\u2009years were thawed, paired, and divided into two groups: one with a centrifugation step to remove glycerol before deglycerolization (\u201cvolume reduction\u201d) and one without. Biochemical and hematological parameters assessed included hemolysis, hematocrit, hemoglobin, ATP, pH, and osmolality. The protocol was then validated.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Hemolysis rates were lower with than without volume reduction (0.4% vs. 0.6%). Centrifuged RBCs also showed higher recovery (72% vs. 63%), increased hematocrit (0.51\u2009L\/L vs. 0.40\u2009L\/L), and improved pH stability (6.17 vs. 6.11). In the validation phase, six RBC units deglycerolized using the volume reduction step met Canadian Standards Association requirements for hematocrit, hemoglobin, hemolysis, and sterility.<\/jats:p><\/jats:sec><jats:sec><jats:title>Discussion<\/jats:title><jats:p>We optimized and validated a new protocol leveraging the ACP\u00ae 215 cell washer to deglycerolize Meryman\u2010frozen RBCs. This method yielded low hemolysis, acceptable pH, and satisfactory recovery, especially with prior glycerol removal by centrifugation. The protocol has been successfully implemented, and Meryman\u2010frozen RBC units have since been reliably thawed, meeting regulatory standards and supporting hospital needs.<\/jats:p><\/jats:sec>","DOI":"10.1111\/trf.18211","type":"journal-article","created":{"date-parts":[[2025,3,14]],"date-time":"2025-03-14T08:48:57Z","timestamp":1741942137000},"page":"732-739","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Automated processing of Meryman\u2010frozen red blood cells: A novel protocol for deglycerolization"],"prefix":"10.1111","volume":"65","author":[{"given":"Audrey","family":"Laforce\u2010Lavoie","sequence":"first","affiliation":[{"name":"H\u00e9ma\u2010Qu\u00e9bec Medical Affairs and Innovation  Qu\u00e9bec Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jessica","family":"Constanzo\u2010Yanez","sequence":"additional","affiliation":[{"name":"H\u00e9ma\u2010Qu\u00e9bec Reference Laboratory  Montr\u00e9al Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marie\u2010Claire","family":"Chevrier","sequence":"additional","affiliation":[{"name":"H\u00e9ma\u2010Qu\u00e9bec Reference Laboratory  Montr\u00e9al Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0598-8754","authenticated-orcid":false,"given":"Marc","family":"Cloutier","sequence":"additional","affiliation":[{"name":"H\u00e9ma\u2010Qu\u00e9bec Medical Affairs and Innovation  Qu\u00e9bec Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,3,14]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1537-2995.2000.40010110.x"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2004.03271.x"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1423-0410.2000.7930168.x"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tmrv.2004.11.004"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1537-2995.1977.17578014580.x"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2005.00588.x"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2004.04001.x"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1537-2995.2001.41070933.x"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2006.09.029"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.0958-7578.2004.00472.x"},{"key":"e_1_2_10_12_1","first-page":"371","volume-title":"AABB technical manual","author":"Cohn CS","year":"2020"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/vox.12219"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/0005-2736(95)00279-0"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.transci.2005.11.010"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.18156"}],"container-title":["Transfusion"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/trf.18211","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,17]],"date-time":"2025-04-17T17:49:38Z","timestamp":1744912178000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/trf.18211"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,3,14]]},"references-count":15,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2025,4]]}},"alternative-id":["10.1111\/trf.18211"],"URL":"https:\/\/doi.org\/10.1111\/trf.18211","archive":["Portico"],"relation":{},"ISSN":["0041-1132","1537-2995"],"issn-type":[{"value":"0041-1132","type":"print"},{"value":"1537-2995","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,3,14]]},"assertion":[{"value":"2025-01-06","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-02-28","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-03-14","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}