{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,28]],"date-time":"2026-10-28T00:18:37Z","timestamp":1793146717516,"version":"4.5.0"},"reference-count":21,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2025,4,3]],"date-time":"2025-04-03T00:00:00Z","timestamp":1743638400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Transfusion"],"published-print":{"date-parts":[[2025,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>We report a case of suspected <jats:italic>Pseudomonas fluorescens<\/jats:italic> transfusion\u2010transmitted infection in a 64\u2010year\u2010old female patient with pancreatic adenocarcinoma. The patient developed a biliary obstruction necessitating a drainage catheter, which was complicated by an arterial hemorrhage. Following the transfusion of 1 RBC unit, the patient developed flank pain, chills, and tachycardia.<\/jats:p><\/jats:sec><jats:sec><jats:title>Study Design and Methods<\/jats:title><jats:p>The transfusion reaction workup was negative for hemolytic transfusion reaction. However, Gram stains of the implicated RBC unit revealed Gram\u2010negative rods. Fortunately, the patient was already receiving broad\u2010spectrum antibiotics, and preliminary investigation results were available early enough to alert the medical team and adjust antibiotic coverage. The patient was hospitalized in the ICU where she had elevated WBC counts that normalized after the addition of cefepime. The blood collection center was notified.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Both aerobic and anaerobic cultures incubated at 35\u00b0C were negative for growth. A subculture and incubation at room temperature (25\u00b0C) demonstrated sufficient growth for the identification of <jats:italic>Pseudomonas fluorescens<\/jats:italic> by both Vitek\u2010MS MALDI\u2010TOF and Vitek2 biochemical methods.<\/jats:p><\/jats:sec><jats:sec><jats:title>Discussion<\/jats:title><jats:p><jats:italic>Pseudomonas fluorescens<\/jats:italic> is a Gram\u2010negative rod\u2010shaped bacterium, well\u2010studied as an environmental microbe. It can cause opportunistic infections in humans and was implicated in previous fatal septic transfusion reactions. This report highlights the importance of both standardization in blood product culture protocols and the need for collaboration between microbiology laboratories and transfusion practitioners to optimize the recovery of potentially clinically important fastidious organisms.<\/jats:p><\/jats:sec>","DOI":"10.1111\/trf.18226","type":"journal-article","created":{"date-parts":[[2025,4,3]],"date-time":"2025-04-03T14:39:15Z","timestamp":1743691155000},"page":"1007-1011","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Collaboration is key: Case report of suspected <i>Pseudomonas fluorescens<\/i> transfusion\u2010associated infection"],"prefix":"10.1111","volume":"65","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5127-5069","authenticated-orcid":false,"given":"Nancy","family":"El Beayni","sequence":"first","affiliation":[{"name":"Department of Pathology and Laboratory Medicine University of Miami Miller School of Medicine  Miami Florida USA"},{"name":"Department of Pathology and Laboratory Medicine American University of Beirut Medical Center  Beirut Lebanon"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Octavio","family":"Martinez","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine University of Miami Miller School of Medicine  Miami Florida USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"YanYun","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine University of Miami Miller School of Medicine  Miami Florida USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter","family":"Hosein","sequence":"additional","affiliation":[{"name":"Division of Hematology\/Oncology, University of Miami Miller School of Medicine University of Miami Miller School of Medicine  Miami Florida USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jordan","family":"Colson","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine University of Miami Miller School of Medicine  Miami Florida USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,4,3]]},"reference":[{"key":"e_1_2_5_2_1","unstructured":"FDA.Fatalities Reported to FDA Following Blood Collection and Transfusion. Annual Summary for Fiscal Year 2018.2020."},{"key":"e_1_2_5_3_1","unstructured":"US Food and Drug Administration.Fatalities Reported to FDA Following Blood Collection and Transfusion Annual Summary for FY 2020.2020Available from:https:\/\/www.fda.gov\/media\/160859\/download"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tmrv.2019.01.001"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.16545"},{"key":"e_1_2_5_6_1","unstructured":"U.S. Centers for Disease Control and Prevention.The National Healthcare Safety Network (NHSN) manual: biovigilance com\u2010ponent v2.8.2023."},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1177\/039463201002300120"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/0002-9343(84)90751-4"},{"issue":"42","key":"e_1_2_5_9_1","first-page":"2583","article-title":"Pseudomonas fluorescens septicemia following transfusion of contaminated blood","volume":"13","author":"Gibaud M","year":"1984","journal-title":"Presse Med"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0140-6736(84)90924-3"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/0195-6701(87)90120-4"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1423-0410.1988.tb03905.x"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3148.1992.tb00161.x"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1086\/592968"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.5694\/j.1326-5377.1994.tb126622.x"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/0732-8893(85)90025-2"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1053\/gast.2002.35379"},{"key":"e_1_2_5_18_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00044-14"},{"key":"e_1_2_5_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/vox.13057"},{"key":"e_1_2_5_20_1","doi-asserted-by":"publisher","DOI":"10.3201\/eid2910.230869"},{"key":"e_1_2_5_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.15317"},{"key":"e_1_2_5_22_1","volume-title":"Clinical microbiology procedures handbook, multi\u2010volume","author":"Leber AL","year":"2023"}],"container-title":["Transfusion"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/trf.18226","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,7,28]],"date-time":"2025-07-28T05:54:19Z","timestamp":1753682059000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/trf.18226"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,4,3]]},"references-count":21,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2025,5]]}},"alternative-id":["10.1111\/trf.18226"],"URL":"https:\/\/doi.org\/10.1111\/trf.18226","archive":["Portico"],"relation":{},"ISSN":["0041-1132","1537-2995"],"issn-type":[{"value":"0041-1132","type":"print"},{"value":"1537-2995","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,4,3]]},"assertion":[{"value":"2023-11-15","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-03-10","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-04-03","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}