{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,5]],"date-time":"2026-08-05T00:02:49Z","timestamp":1785888169892,"version":"3.56.0"},"reference-count":25,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2025,4,2]],"date-time":"2025-04-02T00:00:00Z","timestamp":1743552000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100012728","name":"Frederick National Laboratory for Cancer Research","doi-asserted-by":"publisher","award":["75N91024F00011"],"award-info":[{"award-number":["75N91024F00011"]}],"id":[{"id":"10.13039\/100012728","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000050","name":"National Heart, Lung, and Blood Institute","doi-asserted-by":"publisher","award":["1K23HL151826"],"award-info":[{"award-number":["1K23HL151826"]}],"id":[{"id":"10.13039\/100000050","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000054","name":"National Cancer Institute","doi-asserted-by":"publisher","award":["5T32CA193145"],"award-info":[{"award-number":["5T32CA193145"]}],"id":[{"id":"10.13039\/100000054","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000062","name":"National Institute of Diabetes and Digestive and Kidney Diseases","doi-asserted-by":"publisher","award":["R01DK131926"],"award-info":[{"award-number":["R01DK131926"]}],"id":[{"id":"10.13039\/100000062","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100006492","name":"Division of Intramural Research","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100006492","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000005","name":"U.S. Department of Defense","doi-asserted-by":"publisher","award":["W81XWH1810742"],"award-info":[{"award-number":["W81XWH1810742"]}],"id":[{"id":"10.13039\/100000005","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Transfusion"],"published-print":{"date-parts":[[2025,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Kaposi's sarcoma herpesvirus (KSHV) causes a life\u2010long infection that can progress to several types of KSHV\u2010associated diseases. There is evidence for transfusion transmission of KSHV. In endemic regions, such as sub\u2010Saharan African, KSHV seroprevalence is &gt;40%. However, previous studies of blood donors utilized immunoassays that detect KSHV\u2010associated disease\u2010specific antigens, which may underestimate the true burden of KSHV in a healthy population.<\/jats:p><\/jats:sec><jats:sec><jats:title>Study Design and Methods<\/jats:title><jats:p>We utilized samples from an on\u2010going transfusion transmitted infection clinical trial to estimate the seroprevalence of KSHV among 4921 blood donations from healthy donors in Uganda collected between October 2019 and December 2022. A multiplexed bead\u2010based assay was used to measure plasma IgG against five antigens encoded by the K8.1, K10.5, ORF73, ORF38, and ORF25 genes of KSHV. Significant associations between donor characteristics and seroprevalence were assessed by chi\u2010square tests.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Overall, KSHV seroprevalence was 69.1%. Seroprevalence was higher in units collected from older donors compared with younger donors and male donors (71.9% [95% confidence interval (CI)\u2009=\u200970.4%\u201373.3%]) compared with female donors (61.3% [95% CI\u2009=\u200958.6%\u201364.0%]; <jats:italic>p<\/jats:italic>\u2009&lt;\u2009.001). KSHV seroprevalnce was higher among units collected from donors positive for <jats:italic>T. pallidum<\/jats:italic> (82.5% [95% CI\u2009=\u200973.8%\u201389.3%]) compared with units collected from donors who were negative (68.8% [95% CI\u2009=\u200967.5%\u201370.1%]; <jats:italic>p<\/jats:italic>\u2009&lt;\u2009.001). KSHV seroprevalence was higher in units that tested positive for HIV, HBV, or HCV, though these results were not statistically significant.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Given the high seroprevalence and limited availability of lab assays that detect active KSHV infections, methods such as leukoreduction or pathogen reduction should be considered to potentially reduce the risk of transfusion transmission of KSHV.<\/jats:p><\/jats:sec>","DOI":"10.1111\/trf.18236","type":"journal-article","created":{"date-parts":[[2025,4,4]],"date-time":"2025-04-04T06:32:32Z","timestamp":1743748352000},"page":"827-833","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Kaposi's sarcoma herpesvirus seroprevalence among blood donors in Uganda"],"prefix":"10.1111","volume":"65","author":[{"given":"Tait","family":"Huso","sequence":"first","affiliation":[{"name":"Department of Pathology, School of Medicine Johns Hopkins University  Baltimore Maryland USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jodie L.","family":"White","sequence":"additional","affiliation":[{"name":"Department of Pathology, School of Medicine Johns Hopkins University  Baltimore Maryland USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dorothy","family":"Kyeyune","sequence":"additional","affiliation":[{"name":"Uganda Blood Transfusion Services  Kampala Uganda"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Angela","family":"D'Adamo","sequence":"additional","affiliation":[{"name":"Department of Pathology, School of Medicine Johns Hopkins University  Baltimore Maryland USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nazzarena","family":"Labo","sequence":"additional","affiliation":[{"name":"Viral Oncology Section, AIDS and Cancer Virus Program, Frederick National Laboratory for Cancer Research  Frederick Maryland USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wendell","family":"Miley","sequence":"additional","affiliation":[{"name":"Viral Oncology Section, AIDS and Cancer Virus Program, Frederick National Laboratory for Cancer Research  Frederick Maryland USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ezra","family":"Musisi","sequence":"additional","affiliation":[{"name":"Uganda Blood Transfusion Services  Kampala Uganda"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Khan","family":"Moses","sequence":"additional","affiliation":[{"name":"Uganda Blood Transfusion Services  Kampala Uganda"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ronnie","family":"Kasirye","sequence":"additional","affiliation":[{"name":"MUJHU Research Collaboration  Kampala Uganda"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Irene","family":"Lubega","sequence":"additional","affiliation":[{"name":"MUJHU Research Collaboration  Kampala Uganda"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hellen","family":"Wambongo Musana","sequence":"additional","affiliation":[{"name":"MUJHU Research Collaboration  Kampala Uganda"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Priscilla","family":"Eroju","sequence":"additional","affiliation":[{"name":"MUJHU Research Collaboration  Kampala Uganda"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mahnaz","family":"Motevalli","sequence":"additional","affiliation":[{"name":"Department of Pathology, School of Medicine Johns Hopkins University  Baltimore Maryland USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5945-4571","authenticated-orcid":false,"given":"Raymond","family":"Goodrich","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Immunology and Pathology Colorado State University  Fort Collins Colorado USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. 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