{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,8,2]],"date-time":"2025-08-02T18:54:55Z","timestamp":1754160895406,"version":"3.41.2"},"reference-count":6,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2025,4,7]],"date-time":"2025-04-07T00:00:00Z","timestamp":1743984000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100012023","name":"Stichting Sanquin Bloedvoorziening","doi-asserted-by":"publisher","award":["PPOC22\u201014\/L2714 (to Rick Kapur)"],"award-info":[{"award-number":["PPOC22\u201014\/L2714 (to Rick Kapur)"]}],"id":[{"id":"10.13039\/501100012023","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Transfusion"],"published-print":{"date-parts":[[2025,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Heparin\u2010induced thrombocytopenia (HIT) is a severe complication characterized by thrombocytopenia and thrombosis. The presence of antibodies against heparin\/platelet factor 4 (PF4) complexes is a key indicator for HIT. Diagnostic laboratory testing generally includes detection of anti\u2010heparin\/PF4 and\/or functional testing such as the heparin\u2010induced platelet activation assay (HIPAA). However, current functional tests are time\u2010consuming and require specific technical skills. The PF4\u2010dependent P\u2010selectin expression assay (PEA) is a flow cytometry\u2010based test that evaluates activation of donor platelets in the presence of HIT patient serum.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We conducted the PEA with 23 patient sera that were positive in both the anti\u2010PF4\/heparin enzyme\u2010linked immunosorbent assay (ELISA) and HIPAA and 26 sera that tested negative in the anti\u2010PF4\/heparin ELISA. We next compared the PEA to the HIPAA using a retrospective clinical cohort of 195 sera of suspected HIT patients and tested the reproducibility of the PEA.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The PEA was found to have a sensitivity of 73.9% and a specificity of 73.1% compared to the HIPAA. In our retrospective clinical cohort, we found that 74.4% (145 out of 195 samples) had identical results in both the HIPAA and PEA, with a specificity of 64.94% and sensitivity of 80.51% for the PEA compared to the HIPAA. Reproducibility testing of the PEA across three independent runs demonstrated consistent results in 83.3% (30 out of 36 samples).<\/jats:p><\/jats:sec><jats:sec><jats:title>Discussion<\/jats:title><jats:p>These findings suggest that the PEA is a promising functional test for HIT laboratory diagnostics based on the relatively high sensitivity; however, further studies are needed to validate its clinical applicability.<\/jats:p><\/jats:sec>","DOI":"10.1111\/trf.18242","type":"journal-article","created":{"date-parts":[[2025,4,7]],"date-time":"2025-04-07T07:02:43Z","timestamp":1744009363000},"page":"848-850","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["<scp>PF4<\/scp>\u2010dependent P\u2010selectin expression assay in comparison to the heparin\u2010induced platelet activation assay for the diagnosis of heparin\u2010induced thrombocytopenia"],"prefix":"10.1111","volume":"65","author":[{"given":"Romy T.","family":"Meier","sequence":"first","affiliation":[{"name":"Sanquin Blood Supply Foundation, Department Research and Amsterdam UMC location University of Amsterdam, Landsteiner Laboratory, Meibergdreef 9  Amsterdam The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anne\u2010Tess","family":"Jolink","sequence":"additional","affiliation":[{"name":"Sanquin Blood Supply Foundation, Department Research and Amsterdam UMC location University of Amsterdam, Landsteiner Laboratory, Meibergdreef 9  Amsterdam The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michelle","family":"Kempe","sequence":"additional","affiliation":[{"name":"Sanquin Blood Supply Foundation, Department Research and Amsterdam UMC location University of Amsterdam, Landsteiner Laboratory, Meibergdreef 9  Amsterdam The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9553-9081","authenticated-orcid":false,"given":"Brian R.","family":"Curtis","sequence":"additional","affiliation":[{"name":"Platelet and Neutrophil Immunology Laboratory Blood Research Institute, Versiti Blood Center of Wisconsin  Milwaukee Wisconsin USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Suzanne","family":"Hofstede\u2010van Egmond","sequence":"additional","affiliation":[{"name":"Sanquin Diagnostic Services, Department of Immunohematology Diagnostics  Sanquin The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masja","family":"de Haas","sequence":"additional","affiliation":[{"name":"Department of Hematology Leiden University Medical Center  Leiden The Netherlands"},{"name":"Sanquin Blood Supply Foundation, Department Research  Amsterdam The Netherlands"},{"name":"Medical Affairs  Sanquin Amsterdam The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Leendert","family":"Porcelijn","sequence":"additional","affiliation":[{"name":"Sanquin Diagnostic Services, Department of Immunohematology Diagnostics  Sanquin The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1608-876X","authenticated-orcid":false,"given":"Rick","family":"Kapur","sequence":"additional","affiliation":[{"name":"Sanquin Blood Supply Foundation, Department Research and Amsterdam UMC location University of Amsterdam, Landsteiner Laboratory, Meibergdreef 9  Amsterdam The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,4,7]]},"reference":[{"key":"e_1_2_2_2_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2016-11-709873"},{"key":"e_1_2_2_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/bjh.19180"},{"key":"e_1_2_2_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chest.2016.02.641"},{"key":"e_1_2_2_5_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2014-06-580894"},{"key":"e_1_2_2_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/jth.15233"},{"key":"e_1_2_2_7_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2012-07-443051"}],"container-title":["Transfusion"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/trf.18242","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,7,28]],"date-time":"2025-07-28T05:54:12Z","timestamp":1753682052000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/trf.18242"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,4,7]]},"references-count":6,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2025,5]]}},"alternative-id":["10.1111\/trf.18242"],"URL":"https:\/\/doi.org\/10.1111\/trf.18242","archive":["Portico"],"relation":{},"ISSN":["0041-1132","1537-2995"],"issn-type":[{"type":"print","value":"0041-1132"},{"type":"electronic","value":"1537-2995"}],"subject":[],"published":{"date-parts":[[2025,4,7]]},"assertion":[{"value":"2025-01-29","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication 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