{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,29]],"date-time":"2026-08-29T01:26:40Z","timestamp":1787966800998,"version":"build-2784847793"},"reference-count":29,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2025,4,16]],"date-time":"2025-04-16T00:00:00Z","timestamp":1744761600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["P30CA15083"],"award-info":[{"award-number":["P30CA15083"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["U01CA271410"],"award-info":[{"award-number":["U01CA271410"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Transfusion"],"published-print":{"date-parts":[[2025,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Tandem mass spectrometry (MS\/MS) has become a common clinical laboratory testing modality has demonstrated success in distinguishing between small protein variations in transthyretin amyloidosis. Since many common clinically significant RBC antigens are also small protein variations, this study aimed to determine if MS\/MS could correctly detect common RBC antigens within the Rh, Kell, Duffy, MNS, Kidd, Diego, and Lutheran blood group systems.<\/jats:p><\/jats:sec><jats:sec><jats:title>Study Design and Methods<\/jats:title><jats:p>Residual samples from serotyped\/genotyped blood donors at a hospital\u2010based blood donation center from February to August 2021 were analyzed. RBC membrane protein preparations underwent protease digestion prior to MS\/MS analysis for RhD, RhCE, Kell, atypical chemokine receptor 1 (Duffy), glycophorin A (MNS), glycophorin B (MNS), urea transporter 1 (Kidd), band 3 anion transport protein (Diego), and basal cell adhesion molecule (Lutheran). Untargeted liquid chromatography (LC)\u2010MS\/MS detected protein\u2010specific peptides, while targeted LC\u2010selected reaction monitoring (LC\u2010SRM) detected RBC antigen\u2010containing peptides.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Through the use of multiple proteases, untargeted LC\u2013MS\/MS detected protein\u2010specific peptides in all but glycophorin B, with band 3 anion transport protein, basal cell adhesion molecule, Kell, and glycophorin A having the greatest protein sequence coverage. Targeted LC\u2010SRM detected antigen\u2010specific peptides for C<jats:sup>w<\/jats:sup>, Di<jats:sup>a<\/jats:sup>\/Di<jats:sup>b<\/jats:sup>, Kp<jats:sup>b<\/jats:sup>, and Wr<jats:sup>a<\/jats:sup>\/Wr<jats:sup>b<\/jats:sup>, which agreed with the donors' previous typing results.<\/jats:p><\/jats:sec><jats:sec><jats:title>Discussion<\/jats:title><jats:p>MS\/MS can successfully detect peptides from several blood group systems but only detected a subset of their common RBC antigens. Further sample enrichment and MS\/MS detection improvements will need to occur before MS\/MS could be considered a clinical RBC phenotyping modality.<\/jats:p><\/jats:sec>","DOI":"10.1111\/trf.18252","type":"journal-article","created":{"date-parts":[[2025,4,16]],"date-time":"2025-04-16T08:24:17Z","timestamp":1744791857000},"page":"968-977","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Detecting red blood cell protein antigens by tandem mass spectrometry"],"prefix":"10.1111","volume":"65","author":[{"given":"Sandip","family":"Chavan","sequence":"first","affiliation":[{"name":"Department of Laboratory Medicine &amp; Pathology Mayo Clinic  Rochester Minnesota USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ramanath","family":"Majumdar","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine &amp; Pathology Mayo Clinic  Rochester Minnesota USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9232-1589","authenticated-orcid":false,"given":"Neha","family":"Joshi","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine &amp; Pathology Mayo Clinic  Rochester Minnesota USA"},{"name":"Manipal Academy of Higher Education  Manipal Karnataka India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Benjamin","family":"Madden","sequence":"additional","affiliation":[{"name":"Proteomics Core Mayo Clinic  Rochester Minnesota USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jane","family":"Peterson","sequence":"additional","affiliation":[{"name":"Proteomics Core Mayo Clinic  Rochester Minnesota USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2692-6613","authenticated-orcid":false,"given":"Craig T.","family":"Tauscher","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine &amp; Pathology Mayo Clinic  Rochester Minnesota USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jennifer E.","family":"Schieber","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine &amp; Pathology Mayo Clinic  Rochester Minnesota USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew P.","family":"Norgan","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine &amp; Pathology Mayo Clinic  Rochester Minnesota USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eapen K.","family":"Jacob","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine &amp; Pathology Mayo Clinic  Rochester Minnesota USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Akhilesh","family":"Pandey","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine &amp; Pathology Mayo Clinic  Rochester Minnesota USA"},{"name":"Manipal Academy of Higher Education  Manipal Karnataka India"},{"name":"Center for Individualized Medicine Mayo Clinic  Rochester Minnesota USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7868-2852","authenticated-orcid":false,"given":"Justin E.","family":"Juskewitch","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine &amp; Pathology Mayo Clinic  Rochester Minnesota USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,4,16]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"crossref","unstructured":"U.S. Code of Federal Regulations Title 42 2003:1649.","DOI":"10.1117\/1.1572889"},{"key":"e_1_2_8_3_1","unstructured":"U.S. Department of Health and Human Services Food and Drug Administration Center for Biologics Evaluation and Research.Labeling of Red Blood Cell Units with Historical Antigen Typing Results.2008."},{"key":"e_1_2_8_4_1","unstructured":"AABB Standards 5.15 and 5.16: Blood Banks and Transfusion Services: 33rd Edition 2022."},{"key":"e_1_2_8_5_1","doi-asserted-by":"crossref","unstructured":"LaneWJ.Recent advances in blood group genotyping. 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