{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T15:05:10Z","timestamp":1789657510950,"version":"4.0.1"},"reference-count":38,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2025,5,5]],"date-time":"2025-05-05T00:00:00Z","timestamp":1746403200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,5,5]],"date-time":"2025-05-05T00:00:00Z","timestamp":1746403200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100015539","name":"Australian Government","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100015539","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Transfusion"],"published-print":{"date-parts":[[2025,6]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Rare phenotype red blood cells (RBCs) are routinely cryopreserved and stored at \u221280\u00b0C for over 10\u2009years. Upon thawing and deglycerolization, RBCs resuspended in saline\u2010adenosine\u2010glucose\u2010mannitol (SAGM) have a limited 24\u2010h shelf\u2010life. There is a lack of international consensus as to whether irradiation of deglycerolized RBCs is necessary for patients at risk of transfusion\u2010associated graft\u2010versus\u2010host disease. The aim of this study was to evaluate the quality of X\u2010ray irradiated deglycerolized RBCs resuspended in SAGM.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Study design and methods<\/jats:title>\n                    <jats:p>\n                      Matched RBC components (\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u200921 pairs) were glycerolized with 40% glycerol, frozen at \u221280\u00b0C, deglycerolized using an ACP\u2010215 cell washer, and resuspended in SAGM. One of each pair was X\u2010ray irradiated post\u2010deglycerolization, while the other remained untreated (control). RBCs were sampled pre\u2010freeze and immediately, 24\u2010, 48\u2010, and 72\u2010h post\u2010irradiation. Quality indicators including RBC indices, metabolic, and biochemical parameters were measured.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      There were no significant differences between X\u2010irradiated and control groups for volume, hemoglobin (Hb), hematocrit, or supernatant Hb immediately post\u2010thaw. Hemolysis was higher in X\u2010irradiated deglycerolized RBCs (0.35\u2009\u00b1 0.14%) than controls (0.32\u2009\u00b1\u20090.05%) after 24\u2010h (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.077). Potassium (K\n                      <jats:sup>+<\/jats:sup>\n                      ) release was significantly higher in X\u2010irradiated RBCs compared to controls after 24\u2010h (6.26\u2009\u00b1\u20090.95\u2009mmol\/unit vs. 3.21\u2009\u00b1\u20091.01\u2009mmol\/unit respectively;\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u2009.001).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Discussion<\/jats:title>\n                    <jats:p>\n                      X\u2010irradiation had a significant effect on K\n                      <jats:sup>+<\/jats:sup>\n                      release from deglycerolized RBCs during storage, and to a lesser extent, hemolysis. The K\n                      <jats:sup>+<\/jats:sup>\n                      and hemolysis levels at 24\u2009h post\u2010irradiation were acceptable, as were the other quality parameters, indicating that X\u2010irradiation of these components is feasible.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/trf.18274","type":"journal-article","created":{"date-parts":[[2025,5,5]],"date-time":"2025-05-05T09:43:45Z","timestamp":1746438225000},"page":"1124-1131","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Evaluating the quality of X\u2010ray irradiated deglycerolized red blood cells stored in saline\u2010adenosine\u2010glucose\u2010mannitol additive solution"],"prefix":"10.1111","volume":"65","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8423-3618","authenticated-orcid":false,"given":"Kelly M.","family":"Winter","sequence":"first","affiliation":[{"name":"Research and Development 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