{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,24]],"date-time":"2026-03-24T11:56:23Z","timestamp":1774353383866,"version":"3.50.1"},"reference-count":42,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2025,8,3]],"date-time":"2025-08-03T00:00:00Z","timestamp":1754179200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2025,8,3]],"date-time":"2025-08-03T00:00:00Z","timestamp":1754179200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100000014","name":"Canadian Blood Services","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000014","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000008","name":"Health Canada","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000008","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Transfusion"],"published-print":{"date-parts":[[2025,10]]},"abstract":"Abstract<\/jats:title>\n \n Background<\/jats:title>\n \n Cutibacterium acnes<\/jats:italic>\n contaminated platelet concentrates (PCs) are transfused due to late detection during culture\u2010based screening.\n C. acnes<\/jats:italic>\n has been implicated in mild adverse transfusion reactions; however, it harbors multiple virulence genes and can cause chronic infections in the clinical setting. Since the PC storage environment enhances virulence gene expression in bacteria like\n Staphylococcus aureus<\/jats:italic>\n , this study aimed to evaluate the impact of PC storage on\n C. acnes<\/jats:italic>\n virulence and its potential to cause chronic infections.\n <\/jats:p>\n <\/jats:sec>\n \n Study design and methods<\/jats:title>\n \n PCs and media were inoculated with\n C. acnes<\/jats:italic>\n (BPNBT\u201019195 and BPNBT\u201019329) and\n S. aureus<\/jats:italic>\n (CBS 2016\u201005, control) PC isolates. Following incubation for 5\u2009days (20\u201324\u00b0C\/agitation), bacterial virulence was compared in the\n Bombyx mori<\/jats:italic>\n (silkworm) model. Additionally, silkworm innate response (hemolymph melanization and superoxide dismutase activity (SOD)), adhesion to HEK293T epithelial cells, and differential expression of virulence genes involved in tissue invasion (\n hyl<\/jats:italic>\n ,\n mce<\/jats:italic>\n ) and persistence (\n roxP<\/jats:italic>\n , lipase) were assessed in\n C. acnes<\/jats:italic>\n samples.\n <\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n PC\u2010derived\n C. acnes<\/jats:italic>\n caused significantly lower larval mortality, hemolymph melanization, and SOD activity compared to media\u2010derived counterparts; conversely, PC\u2010derived\n S. aureus<\/jats:italic>\n caused significantly higher larval mortality. Furthermore, PC\u2010derived\n C. acnes<\/jats:italic>\n displayed higher adherence to HEK293T cells and heightened virulence gene expression compared to media controls.\n <\/jats:p>\n <\/jats:sec>\n \n Discussion<\/jats:title>\n \n Our data demonstrated that the PC milieu enhances the ability of\n C. acnes<\/jats:italic>\n to adhere to mammalian epithelia and promotes the expression of genes involved in invasion and persistence in host tissue, potentially priming this bacterium to cause chronic infections in transfusion patients, which warrants further investigation.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1111\/trf.18368","type":"journal-article","created":{"date-parts":[[2025,8,4]],"date-time":"2025-08-04T06:35:51Z","timestamp":1754289351000},"page":"1851-1860","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["The platelet concentrate storage environment may enhance the ability of\n Cutibacterium acnes<\/i>\n to establish chronic infections"],"prefix":"10.1111","volume":"65","author":[{"given":"Dilini","family":"Kumaran","sequence":"first","affiliation":[{"name":"Donation Policy & Studies Canadian Blood Services Ottawa Ontario Canada"},{"name":"Biochemistry, Microbiology and Immunology University of Ottawa Ottawa Ontario Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0705-4987","authenticated-orcid":false,"given":"Sandra","family":"Ramirez\u2010Arcos","sequence":"additional","affiliation":[{"name":"Donation Policy & Studies Canadian Blood Services Ottawa Ontario Canada"},{"name":"Biochemistry, Microbiology and Immunology University of Ottawa Ottawa Ontario 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