{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,17]],"date-time":"2026-02-17T08:08:31Z","timestamp":1771315711275,"version":"3.50.1"},"reference-count":52,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2025,9,12]],"date-time":"2025-09-12T00:00:00Z","timestamp":1757635200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,9,12]],"date-time":"2025-09-12T00:00:00Z","timestamp":1757635200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100000780","name":"European Commission","doi-asserted-by":"publisher","award":["101015756"],"award-info":[{"award-number":["101015756"]}],"id":[{"id":"10.13039\/501100000780","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002347","name":"Bundesministerium f\u00fcr Bildung und Forschung","doi-asserted-by":"publisher","award":["16LW0108"],"award-info":[{"award-number":["16LW0108"]}],"id":[{"id":"10.13039\/501100002347","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Transfusion"],"published-print":{"date-parts":[[2025,10]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>COVID\u201019 convalescent plasma (CCP) is a treatment option for COVID\u201019. Understanding of donor and product characteristics is important for optimization of CCP therapy. We report the experience of collection of very high\u2010titer CCP for the trial \u201cA randomized open\u2010label trial of early, very high\u2010titer convalescent plasma therapy in clinically vulnerable individuals with mild COVID\u201019\u201d (COVIC\u201019) (NCT05271929).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Study Design and Methods<\/jats:title>\n                    <jats:p>Individuals who had recovered from COVID\u201019 and had \u22651 dose of a SARS\u2010CoV\u20102 vaccine were recruited as donors for CCP. Anti\u2010SARS\u2010CoV\u20102 antibodies were measured by ELISA, and neutralization capacity against SARS\u2010CoV\u20102 variants was assessed in surrogate and pseudovirus\u2010neutralization assays. Correlation of antibody titers with donor characteristics and antibody kinetics was analyzed.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>We recruited 688 potential donors. 41.4% of individuals had antibody concentrations of \u22654000 BAU\/ml (anti\u2010SARS\u2010CoV\u20102 IgG ELISA [QuantiVac]). Concentrations did not significantly differ by sex or ABO type, but were higher among those who had received at least three vaccinations. Highest titers were observed in those with a breakthrough infection after two vaccinations, followed by a booster (median 5374 BAU\/mL) or breakthrough infection after the 3rd or 4th vaccination (median 3846 BAU\/mL). Ultimately, 172 eligible individuals donated CCP with a median concentration of 6858 BAU\/mL (range 4015\u201322,923 BAU\/mL).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Discussion<\/jats:title>\n                    <jats:p>We demonstrate the feasibility of the collection of very high\u2010titer CCP products under a harmonized protocol for a randomized clinical trial, but it requires substantial donor selection, appropriate antibody assays, rapid succession of screening, and apheresis to take advantage of the short period of very high antibody concentrations.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/trf.18394","type":"journal-article","created":{"date-parts":[[2025,9,12]],"date-time":"2025-09-12T14:20:54Z","timestamp":1757686854000},"page":"1888-1902","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["<scp>SARS<\/scp>\n                    \u2010\n                    <scp>CoV<\/scp>\n                    \u20102 convalescent plasma for the controlled clinical trial \u201c\n                    <scp>COVIC<\/scp>\n                    \u201019\u201d: Experience from collection of very high\u2010titer plasma from superimmunized individuals"],"prefix":"10.1111","volume":"65","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3690-2498","authenticated-orcid":false,"given":"Simone","family":"Hoffmann","sequence":"first","affiliation":[{"name":"Institute of Clinical Transfusion Medicine and Immunogenetics Ulm, German Red Cross Blood Transfusion Service Baden\u2010W\u00fcrttemberg\u2010Hessen and University Hospital Ulm; and Institute of Transfusion Medicine, University Ulm  Ulm Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alina","family":"Seidel","sequence":"additional","affiliation":[{"name":"Institute of Molecular Virology Ulm University Medical Center  Ulm Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carolin","family":"Ludwig","sequence":"additional","affiliation":[{"name":"Institute of Clinical Transfusion Medicine and Immunogenetics Ulm, German Red Cross Blood Transfusion Service Baden\u2010W\u00fcrttemberg\u2010Hessen and University Hospital Ulm; and Institute of Transfusion Medicine, University Ulm  Ulm Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christiane","family":"Vieweg","sequence":"additional","affiliation":[{"name":"Institute of Clinical Transfusion Medicine and Immunogenetics Ulm, German Red Cross Blood Transfusion Service Baden\u2010W\u00fcrttemberg\u2010Hessen and University Hospital Ulm; and Institute of Transfusion Medicine, University Ulm  Ulm Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rebecca","family":"M\u00fcller","sequence":"additional","affiliation":[{"name":"Institute of Transfusion Medicine and Immunology Medical Faculty Mannheim, Heidelberg University, German Red Cross Blood Transfusion Service Baden\u2010W\u00fcrttemberg \u2013 Hessen  Mannheim Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Henrike","family":"Hofmann","sequence":"additional","affiliation":[{"name":"Institute of Clinical Transfusion Medicine and Immunogenetics Ulm, German Red Cross Blood Transfusion Service Baden\u2010W\u00fcrttemberg\u2010Hessen and University Hospital Ulm; and Institute of Transfusion Medicine, University Ulm  Ulm Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bernd","family":"Jahrsd\u00f6rfer","sequence":"additional","affiliation":[{"name":"Institute of Clinical Transfusion Medicine and Immunogenetics Ulm, German Red Cross Blood Transfusion Service Baden\u2010W\u00fcrttemberg\u2010Hessen and University Hospital Ulm; and Institute of Transfusion Medicine, University Ulm  Ulm Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Patrick","family":"Wuchter","sequence":"additional","affiliation":[{"name":"Institute of Transfusion Medicine and Immunology Medical Faculty Mannheim, Heidelberg University, German Red Cross Blood Transfusion Service Baden\u2010W\u00fcrttemberg \u2013 Hessen  Mannheim Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Harald","family":"Kl\u00fcter","sequence":"additional","affiliation":[{"name":"Institute of Transfusion Medicine and Immunology Medical Faculty Mannheim, Heidelberg University, German Red Cross Blood Transfusion Service Baden\u2010W\u00fcrttemberg \u2013 Hessen  Mannheim Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9338-0900","authenticated-orcid":false,"given":"Michael","family":"Schmidt","sequence":"additional","affiliation":[{"name":"Institute of Transfusion Medicine and Immunohematology German Red Cross Blood Transfusion Service Baden\u2010W\u00fcrttemberg \u2013 Hessen  Frankfurt Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Matthias","family":"Johnsen","sequence":"additional","affiliation":[{"name":"Institute of Transfusion Medicine Dresden German Red Cross Blood Transfusion Service Nord\u2010Ost gGmbH  Dresden Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thomas","family":"Burkhardt","sequence":"additional","affiliation":[{"name":"Institute of Transfusion Medicine Plauen German Red Cross Blood Transfusion Service Nord\u2010Ost gGmbH  Plauen Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thomas","family":"Appl","sequence":"additional","affiliation":[{"name":"Institute of Clinical Transfusion Medicine and Immunogenetics Ulm, German Red Cross Blood Transfusion Service Baden\u2010W\u00fcrttemberg\u2010Hessen and University Hospital Ulm; 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