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Platelet transfusions have been suggested to exacerbate inflammatory conditions, resulting in transfusion\u2010related complications. Here, we used plasma proteomics to study the impact of platelet transfusions under inflammatory conditions.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Plasma proteomics was performed in samples from two studies: (1) 252 samples from healthy volunteers from 36 cases divided into groups (\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u20096) with and without controlled endotoxemia, each with different platelet products, and (2) 54 samples from 27 transfusion events in hemato\u2010oncological patients (\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u200914). Both studies had samples pre\u2010 and post\u2010transfusions.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Controlled endotoxemia elicited a shared inflammatory response across all healthy volunteers. This response was characterized by increased abundance of two kinetically distinct protein clusters, an initial cluster associated with degranulation and inflammation (i.e., S100A9 and MMP9) followed by a cluster of acute\u2010phase proteins (i.e., SAA and CRP). Autologous platelet transfusions in healthy individuals did not induce transfusion\u2010specific changes in plasma protein levels, nor did they exacerbate the effect of controlled endotoxemia. In hemato\u2010oncological patients, we found a transfusion\u2010specific response restricted to alterations in platelet basic protein (PPBP) levels. No additional changes in plasma protein profiles associated with inflammation or dysregulated processes were observed following platelet transfusions.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Discussion<\/jats:title>\n                    <jats:p>Platelet transfusions did not induce specific quantitative changes in plasma protein levels in healthy volunteers. Nonetheless, they were associated with increased levels of PPBP in hemato\u2010oncological patients. In general, no evidence of inflammation or other dysregulated processes was observed following platelet transfusions.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/trf.18397","type":"journal-article","created":{"date-parts":[[2025,9,4]],"date-time":"2025-09-04T05:01:03Z","timestamp":1756962063000},"page":"1825-1838","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Impact of platelet transfusions on plasma proteomes in controlled endotoxemia and in hemato\u2010oncological patients"],"prefix":"10.1111","volume":"65","author":[{"ORCID":"https:\/\/orcid.org\/0009-0008-2001-1205","authenticated-orcid":false,"given":"Eva R.","family":"Smit","sequence":"first","affiliation":[{"name":"Department of Molecular Hematology Sanquin Research  Amsterdam the Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Isabella","family":"Viegen","sequence":"additional","affiliation":[{"name":"Department of Intensive Care Amsterdam UMC location University of Amsterdam  Amsterdam the Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pieter F.","family":"van der Meer","sequence":"additional","affiliation":[{"name":"Department of Hematology Haga Teaching Hospital  The Hague the Netherlands"},{"name":"Department of Product and Process Development Sanquin Blood Bank  Amsterdam the Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4923-2289","authenticated-orcid":false,"given":"Stefan F.","family":"van Wonderen","sequence":"additional","affiliation":[{"name":"Department of Intensive Care Amsterdam UMC location University of Amsterdam  Amsterdam the Netherlands"},{"name":"Laboratory of Experimental Intensive Care and Anesthesiology Amsterdam UMC location University of Amsterdam  Amsterdam the Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4921-8820","authenticated-orcid":false,"given":"Floor L. F.","family":"van Baarle","sequence":"additional","affiliation":[{"name":"Department of Intensive Care Amsterdam UMC location University of Amsterdam  Amsterdam the Netherlands"},{"name":"Laboratory of Experimental Intensive Care and Anesthesiology Amsterdam UMC location University of Amsterdam  Amsterdam the Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1250-7979","authenticated-orcid":false,"given":"Paula F.","family":"Ypma","sequence":"additional","affiliation":[{"name":"Department of Hematology Haga Teaching Hospital  The Hague the Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jean\u2010Louis H.","family":"Kerkhoffs","sequence":"additional","affiliation":[{"name":"Department of Hematology Haga Teaching Hospital  The Hague the Netherlands"},{"name":"Unit Transfusion Medicine Sanquin Blood Bank  Amsterdam The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jan","family":"Voorberg","sequence":"additional","affiliation":[{"name":"Department of Molecular Hematology Sanquin Research  Amsterdam the Netherlands"},{"name":"Department of Experimental Vascular Medicine Amsterdam UMC location University of Amsterdam  Amsterdam the Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3453-7186","authenticated-orcid":false,"given":"Alexander P. 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