{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,12]],"date-time":"2026-06-12T03:51:18Z","timestamp":1781236278258,"version":"3.54.1"},"reference-count":51,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2025,10,30]],"date-time":"2025-10-30T00:00:00Z","timestamp":1761782400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2025,10,30]],"date-time":"2025-10-30T00:00:00Z","timestamp":1761782400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Transfusion"],"published-print":{"date-parts":[[2025,12]]},"abstract":"Abstract<\/jats:title>\n \n \n Background<\/jats:styled-content>\n <\/jats:title>\n \n Refrigerated storage of red cell concentrate (\n RCC<\/jats:styled-content>\n ) leads to metabolic, oxidative, and structural changes that impair functionality and viability. These changes can be attenuated by hypoxic storage.\n <\/jats:p>\n <\/jats:sec>\n \n \n Objective<\/jats:styled-content>\n <\/jats:title>\n \n This study assessed the quality of leucocytes\u2010reduced (\n LR<\/jats:styled-content>\n ),\n \n O\n 2<\/jats:sub>\n <\/jats:styled-content>\n \/\n \n CO\n 2<\/jats:sub>\n <\/jats:styled-content>\n reduced red blood cells (\n RBC<\/jats:styled-content>\n ) stored for 42\u2009days after pre\u2010storage\n \n O\n 2<\/jats:sub>\n <\/jats:styled-content>\n \/\n \n CO\n 2<\/jats:sub>\n <\/jats:styled-content>\n reduction with the Hemanext\n ONE<\/jats:styled-content>\n System, to verify compliance with regional and blood center acceptance criteria across six sites.\n <\/jats:p>\n <\/jats:sec>\n \n \n Study design and methods<\/jats:styled-content>\n <\/jats:title>\n \n Validation studies of the in vitro performance of the\n LR<\/jats:styled-content>\n ,\n \n O\n 2<\/jats:sub>\n <\/jats:styled-content>\n \/\n \n CO\n 2<\/jats:sub>\n <\/jats:styled-content>\n reduced storage (Hemanext\n ONE<\/jats:styled-content>\n ) System were planned and executed at blood production centers in Italy, Germany, Norway and Switzerland. The endpoint\u2010associated study acceptance criteria included total hematocrit >50%, total hemoglobin \u226540\u2009g and hemolysis <0.8%.\n <\/jats:p>\n <\/jats:sec>\n \n \n Results<\/jats:styled-content>\n <\/jats:title>\n \n In total, 133 whole blood units donated at six blood centers were evaluated after processing with the Hemanext\n ONE<\/jats:styled-content>\n System and after 21 and 42\u2009days of storage. At Day 1 post\u2010processing, total hemoglobin (mean\u2009\u00b1\u2009\n SD<\/jats:styled-content>\n ) was 52.4\u2009g\/unit\u2009\u00b1\u20093.1 and total hematocrit percentage (mean\u2009\u00b1\u2009\n SD<\/jats:styled-content>\n ) was 60.6%\u2009\u00b1\u20097.0 (range, 51.9%\u201381.0%). Hemolysis at Day 42 of storage: overall mean\u2009\u00b1\u2009\n SD<\/jats:styled-content>\n , 0.26%\u2009\u00b1\u20090.14.\n <\/jats:p>\n <\/jats:sec>\n \n \n Discussion<\/jats:styled-content>\n <\/jats:title>\n \n Hypoxic\n RBCs<\/jats:styled-content>\n met regulatory quality criteria regardless of collection and processing modalities. These results indicate that processing of\n RBCs<\/jats:styled-content>\n stored under hypoxic conditions satisfies acceptance criteria for transfusion into patients in six European regional blood centers.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1111\/trf.18408","type":"journal-article","created":{"date-parts":[[2025,10,30]],"date-time":"2025-10-30T07:17:20Z","timestamp":1761808640000},"page":"2306-2315","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["A validation study of the in vitro performance of hypoxic red blood cells for transfusion across centers in Europe"],"prefix":"10.1111","volume":"65","author":[{"given":"Vanessa","family":"Agostini","sequence":"first","affiliation":[{"name":"Transfusion Medicine Department IRCCS Ospedale Policlinico San Martino Genoa Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5895-4259","authenticated-orcid":false,"given":"Reinhard","family":"Henschler","sequence":"additional","affiliation":[{"name":"Institute of Transfusion Medicine, University Hospital and Medical Faculty University of Leipzig Leipzig Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6798-1630","authenticated-orcid":false,"given":"Turid Helen Felli","family":"Lunde","sequence":"additional","affiliation":[{"name":"Department of Immunology and Transfusion Medicine Haukeland University Hospital Bergen Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Einar Kl\u00e6boe","family":"Kristoffersen","sequence":"additional","affiliation":[{"name":"Department of Immunology and Transfusion Medicine Haukeland University Hospital Bergen Norway"},{"name":"Department of Clinical Science University of Bergen Bergen Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Geir","family":"Hetland","sequence":"additional","affiliation":[{"name":"Department of Immunology, Institute of Clinical Medicine University of Oslo Oslo Norway"},{"name":"Department of Immunology and Transfusion Medicine Oslo University Hospital Ullev\u00e5l Oslo Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1689-7628","authenticated-orcid":false,"given":"Elena","family":"Danilova","sequence":"additional","affiliation":[{"name":"Department of Immunology and Transfusion Medicine Oslo University Hospital Ullev\u00e5l Oslo Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Agathe","family":"Martin","sequence":"additional","affiliation":[{"name":"Laboratoire de Pr\u00e9paration Cellulaire et d'Analyses Transfusion Interr\u00e9gionale CRS Epalinges Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9470-0179","authenticated-orcid":false,"given":"Michel","family":"Prudent","sequence":"additional","affiliation":[{"name":"Laboratoire de Recherche sur les Produits Sanguins Transfusion Interr\u00e9gionale CRS Epalinges Switzerland"},{"name":"Centre for Research and Innovation in Clinical Pharmaceutical Sciences Lausanne University Hospital and University of Lausanne Lausanne Switzerland"},{"name":"Institute of Pharmaceutical Sciences of Western Switzerland University of Geneva Geneva Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9865-6969","authenticated-orcid":false,"given":"Giuliano","family":"Grazzini","sequence":"additional","affiliation":[{"name":"Consultant, Hemanext, Inc. 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