{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,23]],"date-time":"2026-03-23T15:16:46Z","timestamp":1774279006939,"version":"3.50.1"},"reference-count":40,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2025,11,20]],"date-time":"2025-11-20T00:00:00Z","timestamp":1763596800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,11,20]],"date-time":"2025-11-20T00:00:00Z","timestamp":1763596800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Transfusion"],"published-print":{"date-parts":[[2025,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>RH phenotype abnormalities, such as double populations or the loss of antigen expression, are frequently observed in the laboratory, especially as a result of blood transfusions or hematopoietic stem cell transplantation. Less commonly, these abnormalities may arise during the development of myeloid malignancies.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Study Design and Methods<\/jats:title>\n                    <jats:p>This study reports on 17 patients with RH phenotype abnormalities that could not be attributed to common causes. As part of the diagnosis or follow\u2010up of their myeloid malignancies, molecular analyses, such as next generation sequencing (NGS) and single nucleotide polymorphism (SNP)\u2013array, were performed.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Molecular analyses revealed abnormalities on chromosome 1p, including biallelic mutations in the\n                      <jats:italic>MPL<\/jats:italic>\n                      and\n                      <jats:italic>CSF3R<\/jats:italic>\n                      genes, as well as a deletion of the 1p region. These findings led to the identification of two molecular mechanisms responsible for the observed immunohematological abnormalities: the CN\u2010LOH (Copy Neutral Loss of Heterozygosity) and the deletion of chromosome 1p.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Discussion<\/jats:title>\n                    <jats:p>\n                      CN\u2010LOH of chromosome 1p, resulting in homozygosity for the\n                      <jats:italic>MPL<\/jats:italic>\n                      mutation, has been previously described in the literature. However, we report here for the first time a link between CN\u2010LOH of chromosome 1p (resulting in homozygosity for the\n                      <jats:italic>CSF3R<\/jats:italic>\n                      mutation) or chromosome 1p deletion and the presence of RH phenotype abnormalities. Furthermore, no alloimmunization was observed despite increased transfusion requirements.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/trf.18482","type":"journal-article","created":{"date-parts":[[2025,11,20]],"date-time":"2025-11-20T13:01:52Z","timestamp":1763643712000},"page":"2389-2397","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Abnormal\n                    <scp>RH<\/scp>\n                    antigen expression in myeloid malignancies with\n                    <scp>\n                      <i>MPL<\/i>\n                    <\/scp>\n                    mutation,\n                    <scp>\n                      <i>CSF3R<\/i>\n                    <\/scp>\n                    mutation, or 1p deletion: Evidence of two distinct mechanisms"],"prefix":"10.1111","volume":"65","author":[{"ORCID":"https:\/\/orcid.org\/0009-0006-2403-9446","authenticated-orcid":false,"given":"R\u00e9mi","family":"Bourgeois","sequence":"first","affiliation":[{"name":"Immunohematology laboratory Etablissement fran\u00e7ais du sang Hauts\u2010de\u2010France\u2010Normandie  Lille France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Laure","family":"Delanoe","sequence":"additional","affiliation":[{"name":"Immunohematology laboratory Etablissement fran\u00e7ais du sang Hauts\u2010de\u2010France\u2010Normandie  Lille France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gauthier","family":"Alluin","sequence":"additional","affiliation":[{"name":"Immunohematology laboratory Etablissement fran\u00e7ais du sang Hauts\u2010de\u2010France\u2010Normandie  Lille France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9170-7487","authenticated-orcid":false,"given":"Ludovic","family":"Firrera","sequence":"additional","affiliation":[{"name":"Immunohematology laboratory Etablissement fran\u00e7ais du sang Hauts\u2010de\u2010France\u2010Normandie  Lille France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Olivier","family":"Nibourel","sequence":"additional","affiliation":[{"name":"Laboratory of Hematology Biology and Pathology Center  Lille France"},{"name":"Cancer Heterogeneity, Plasticity and Resistance to Therapies CNRS UMR9020, Inserm UMR1277  Lille France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nicolas","family":"Duployez","sequence":"additional","affiliation":[{"name":"Laboratory of Hematology Biology and Pathology Center  Lille France"},{"name":"Cancer Heterogeneity, Plasticity and Resistance to Therapies CNRS UMR9020, Inserm UMR1277  Lille France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9451-4277","authenticated-orcid":false,"given":"Elisabeth","family":"Durieux\u2010Roussel","sequence":"additional","affiliation":[{"name":"Immunohematology laboratory Etablissement fran\u00e7ais du sang Hauts\u2010de\u2010France\u2010Normandie  Lille France"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,11,20]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/ajcp\/12.11.545"},{"key":"e_1_2_9_3_1","volume-title":"Les groupes sanguins \u00e9rythrocytaires","author":"Chiaroni J","year":"2024"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.1986.tb02933.x"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1537-2995.1984.24384225031.x"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V50.6.981.981"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1006\/geno.1994.1014"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s004390000237"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.89.22.10925"},{"issue":"6","key":"e_1_2_9_10_1","doi-asserted-by":"crossref","first-page":"500","DOI":"10.1159\/000466667","article-title":"The Inheritance of the Rh blood Groups: I. Frequencies in 1000 unrelated Caucasian Families consisting of 2000 parents and 2806 children","volume":"20","author":"Lewis M","year":"1971","journal-title":"Vox Sang"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-1809.1949.tb02442.x"},{"key":"e_1_2_9_12_1","unstructured":"Les analyses immunoh\u00e9matologiques et leurs applications cliniques.Montrouge Saint\u2010Denis:J. Libbey Eurotext \u00c9tablissement fran\u00e7ais du sang;2011."},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.2022017578"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1214514"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2008-01-131664"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.22034\/APJCP.2016.17.10.4647"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-08-2754"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.advenzreg.2006.01.015"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V112.11.176.176"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2007-05-092304"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2013-02-486050"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2013-02-451757"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.4161\/cbt.10.4.12612"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2010.28.3978"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.24031"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V3.6.689.689"},{"key":"e_1_2_9_27_1","volume-title":"The blood group antigen FactsBook","author":"Reid ME","year":"2012"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1002\/gcc.22350"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2009.21.9840"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2009-10-201848"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.1.5741.131"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V54.3.642.642"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2007-01-068106"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1038\/leu.2008.249"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.14201"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.21307\/immunohematology-2019-533"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0887-7963(90)70247-7"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V82.5.1684.bloodjournal8251684b"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V66.4.990.990"},{"issue":"2","key":"e_1_2_9_40_1","first-page":"268","article-title":"Leukemia\u2010induced alterations of serum glycosyltransferase enzymes","volume":"40","author":"Kuhns WJ","year":"1980","journal-title":"Cancer Res"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1537-2995.1981.21181127486.x"}],"container-title":["Transfusion"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/trf.18482","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/trf.18482","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/trf.18482","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,3,23]],"date-time":"2026-03-23T14:27:47Z","timestamp":1774276067000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/trf.18482"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,11,20]]},"references-count":40,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2025,12]]}},"alternative-id":["10.1111\/trf.18482"],"URL":"https:\/\/doi.org\/10.1111\/trf.18482","archive":["Portico"],"relation":{},"ISSN":["0041-1132","1537-2995"],"issn-type":[{"value":"0041-1132","type":"print"},{"value":"1537-2995","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,11,20]]},"assertion":[{"value":"2025-08-02","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-10-23","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-11-20","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}