{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,18]],"date-time":"2026-05-18T15:06:19Z","timestamp":1779116779763,"version":"3.51.4"},"reference-count":6,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2025,11,6]],"date-time":"2025-11-06T00:00:00Z","timestamp":1762387200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,11,6]],"date-time":"2025-11-06T00:00:00Z","timestamp":1762387200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Transfusion"],"published-print":{"date-parts":[[2026,1]]},"abstract":"Abstract<\/jats:title>\n \n Background<\/jats:title>\n Inadequate compaction of red blood cells (RBC) prior to glycerolization using the ACP\u00ae 215 system may lead to the rejection of cryopreserved units, a situation that poses particular challenges when dealing with rare phenotypes. While repeating the centrifugation step could correct this issue, no data currently exist regarding its impact on RBC quality.<\/jats:p>\n <\/jats:sec>\n \n Study Design and Methods<\/jats:title>\n Pools of 3 ABO\u2010isogroup red cell concentrates (RCC) were divided into three units and subjected to one (standard), two, or three centrifugations before glycerolization using the Valeri method. After storage at \u221280\u00b0C, units were thawed, deglycerolized, and stored for 7\u2009days at 2\u20136\u00b0C in additive solution AS\u20103. Hemolysis, hematocrit, hemoglobin content, recovery, volume, and sterility were evaluated post\u2010deglycerolization.<\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n No significant differences were observed in hemolysis, hematocrit, or hemoglobin content between single, double, or triple centrifugation groups. All values were within quality thresholds. While recovery and final volume showed a downward trend after a third centrifugation, the differences were not statistically significant for recovery, and volume remained operationally acceptable.<\/jats:p>\n <\/jats:sec>\n \n Conclusion<\/jats:title>\n Repeating the initial centrifugation step up to two times before glycerolization does not adversely affect the quality of cryopreserved RBCs processed with the ACP\u00ae 215. These findings support updating standard operating procedures to reduce unnecessary rejection of rare donor units due to initial centrifugation issues.<\/jats:p>\n <\/jats:sec>","DOI":"10.1111\/trf.18486","type":"journal-article","created":{"date-parts":[[2025,11,6]],"date-time":"2025-11-06T13:06:47Z","timestamp":1762434407000},"page":"24-29","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Impact of repeated centrifugation prior to glycerolization on the quality of cryopreserved red blood cells"],"prefix":"10.1111","volume":"66","author":[{"given":"Audrey","family":"Laforce\u2010Lavoie","sequence":"first","affiliation":[{"name":"Medical Affairs and Innovation H\u00e9ma\u2010Qu\u00e9bec Quebec Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nathalie","family":"Dussault","sequence":"additional","affiliation":[{"name":"Medical Affairs and Innovation H\u00e9ma\u2010Qu\u00e9bec Quebec Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marc","family":"Cloutier","sequence":"additional","affiliation":[{"name":"Medical Affairs and Innovation H\u00e9ma\u2010Qu\u00e9bec Quebec Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,11,6]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2005.00588.x"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2004.04001.x"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1537-2995.2001.41070933.x"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.3233\/CH-151965"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2017.00731"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.21307\/immunohematology-2021-012"}],"container-title":["Transfusion"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/trf.18486","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/trf.18486","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/trf.18486","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,18]],"date-time":"2026-05-18T14:09:47Z","timestamp":1779113387000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/trf.18486"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,11,6]]},"references-count":6,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2026,1]]}},"alternative-id":["10.1111\/trf.18486"],"URL":"https:\/\/doi.org\/10.1111\/trf.18486","archive":["Portico"],"relation":{},"ISSN":["0041-1132","1537-2995"],"issn-type":[{"value":"0041-1132","type":"print"},{"value":"1537-2995","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,11,6]]},"assertion":[{"value":"2025-09-19","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-10-26","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-11-06","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}