{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T13:11:22Z","timestamp":1789564282891,"version":"build-2803163510"},"reference-count":31,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2026,1,16]],"date-time":"2026-01-16T00:00:00Z","timestamp":1768521600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2026,1,16]],"date-time":"2026-01-16T00:00:00Z","timestamp":1768521600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100016318","name":"Cerus Corporation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100016318","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001677","name":"Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001677","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100010481","name":"Etablissement Fran\u00e7ais du Sang","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100010481","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Transfusion"],"published-print":{"date-parts":[[2026,2]]},"abstract":"Abstract<\/jats:title>\n \n Background<\/jats:title>\n \n Cryoprecipitate Anti\u2010Haemophilic Factor (\n CRYO<\/jats:styled-content>\n \u2010\n AHF<\/jats:styled-content>\n ) is enriched for fibrinogen,\n VWF<\/jats:styled-content>\n ,\n FVIII<\/jats:styled-content>\n ,\n FXIII<\/jats:styled-content>\n and fibronectin, but has short post\u2010thaw expiration due to risk of transfusion\u2010transmitted infection (\n TTI<\/jats:styled-content>\n ) limiting availability for rapid treatment. Amotosalen\u2010\n UVA<\/jats:styled-content>\n pathogen reduction treatment (A\u2010\n PRT<\/jats:styled-content>\n ) to manufacture pathogen\u2010reduced cryoprecipitated fibrinogen complex (\n PRCFC<\/jats:styled-content>\n ) allows 5\u2010day post\u2010thaw expiration, reduces\n TTI<\/jats:styled-content>\n risk, and facilitates early treatment of hemorrhage.\n <\/jats:p>\n <\/jats:sec>\n \n Aims<\/jats:title>\n \n To evaluate adhesive protein functions in\n PRCFC<\/jats:styled-content>\n and lyophilized\n PRCFC<\/jats:styled-content>\n (\n LIFC<\/jats:styled-content>\n ).\n <\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n \n CRYO<\/jats:styled-content>\n \u2010\n AHF<\/jats:styled-content>\n ,\n PRCFC<\/jats:styled-content>\n ,\n LIFC<\/jats:styled-content>\n , and commercial fibrinogen concentrate (\n CFC<\/jats:styled-content>\n ) were evaluated for platelet adhesion and aggregation in variable shear microfluidic assays.\n <\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n Platelet adhesion kinetics to\n CRYO<\/jats:styled-content>\n \u2010\n AHF<\/jats:styled-content>\n ,\n PRCFC,<\/jats:styled-content>\n and\n LIFC<\/jats:styled-content>\n , under low shear flow (300\u2009s\n \u22121<\/jats:sup>\n ) were conserved. Platelet adhesion to\n CFC<\/jats:styled-content>\n at low shear was reduced due to absence of functional\n VWF<\/jats:styled-content>\n .\n \u03b1IIb\u03b23<\/jats:styled-content>\n integrin\/fibrinogen and\n GPIb<\/jats:styled-content>\n \u2010\n IX<\/jats:styled-content>\n \u2010V\/\n VWF<\/jats:styled-content>\n platelet interactions with\n CRYO<\/jats:styled-content>\n \u2010\n AHF<\/jats:styled-content>\n ,\n PRCFC<\/jats:styled-content>\n , and\n LIFC<\/jats:styled-content>\n were confirmed by abciximab and caplacizumab inhibition, respectively. All fibrinogen sources promoted efficient platelet aggregation. Perfusion of reconstituted plasma\u2010free blood (\n RBC<\/jats:styled-content>\n \u2009+\u2009platelets + various cryoprecipitates) on immobilized\n VWF<\/jats:styled-content>\n \u2010binding peptide (1500\u2009s\n \u22121<\/jats:sup>\n ) showed impaired platelet adhesion to\n CFC<\/jats:styled-content>\n compared to\n PRCFC<\/jats:styled-content>\n and\n CRYO<\/jats:styled-content>\n \u2010\n AHF<\/jats:styled-content>\n . Perfusion of reconstituted blood on collagen (3000\u2009s\n \u22121<\/jats:sup>\n ) indicated\n CRYO<\/jats:styled-content>\n \u2010\n AHF<\/jats:styled-content>\n ,\n PRCFC<\/jats:styled-content>\n and\n LIFC<\/jats:styled-content>\n formed thrombi to similar levels. Platelets treated with A\u2010\n PRT<\/jats:styled-content>\n combined with\n PRCFC<\/jats:styled-content>\n or\n LIFC<\/jats:styled-content>\n retained similar activity to\n CRYO<\/jats:styled-content>\n \u2010\n AHF<\/jats:styled-content>\n for platelet aggregation and thrombus formation on collagen.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusions<\/jats:title>\n \n PRCFC<\/jats:styled-content>\n and\n LIFC<\/jats:styled-content>\n retained critical hemostatic functions of\n VWF<\/jats:styled-content>\n and fibrinogen to support platelet adhesion and aggregation during physiologic shear.\n PRCFC<\/jats:styled-content>\n and\n LIFC<\/jats:styled-content>\n represent a therapeutic option for early treatment of massive hemorrhage.\n <\/jats:p>\n 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