{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T19:03:55Z","timestamp":1790103835116,"version":"4.0.1"},"reference-count":39,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2025,12,16]],"date-time":"2025-12-16T00:00:00Z","timestamp":1765843200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,12,16]],"date-time":"2025-12-16T00:00:00Z","timestamp":1765843200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100000050","name":"National Heart, Lung, and Blood Institute","doi-asserted-by":"publisher","award":["P01\u2010HL046925"],"award-info":[{"award-number":["P01\u2010HL046925"]}],"id":[{"id":"10.13039\/100000050","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000050","name":"National Heart, Lung, and Blood Institute","doi-asserted-by":"publisher","award":["K99 HL156051\u201001"],"award-info":[{"award-number":["K99 HL156051\u201001"]}],"id":[{"id":"10.13039\/100000050","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Transfusion"],"published-print":{"date-parts":[[2026,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Liberal platelet transfusion practices increase neonatal morbidity and mortality. The mechanisms underlying this harm are unknown but may involve the immune rather than hemostatic functions of platelets, as well as the significant differences between adult (transfused) and neonatal platelets, particularly the higher P\u2010selectin surface expression on activated adult platelets. In this study, we investigated the immune\/inflammatory effects of transfusing adult platelets into newborn mice.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Study Design and Methods<\/jats:title>\n                    <jats:p>\n                      Washed platelets from wild\u2010type (WT) or\n                      <jats:italic>P\u2010selectin<\/jats:italic>\n                      <jats:sup>\n                        <jats:italic>\u2212\/\u2212<\/jats:italic>\n                      <\/jats:sup>\n                      adult donors or Tyrode's buffer control were transfused into WT and thrombocytopenic\n                      <jats:italic>c\u2010MPL<\/jats:italic>\n                      <jats:sup>\n                        <jats:italic>\u2212\/\u2212<\/jats:italic>\n                      <\/jats:sup>\n                      pups. Blood was collected 2\u2010 or 4\u2010h post\u2010transfusion to measure a panel of plasma inflammatory cytokines, neutrophil extracellular trap (NET) formation, and the percentage of circulating platelet\u2013monocyte and platelet\u2013neutrophil aggregates (PMAs and PNAs).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Transfusion of adult WT platelets into post\u2010natal Day 10 (P10) and 5 (P5) WT pups increased plasma concentrations of inflammatory cytokines 2\u2010 and 4\u2010h post\u2010transfusion, including interleukin\u20106 (IL\u20106) and Keratinocyte\u2010derived chemokine (KC). Transfusion of WT platelets into P10 thrombocytopenic\n                      <jats:italic>c\u2010MPL<\/jats:italic>\n                      <jats:sup>\n                        <jats:italic>\u2212\/\u2212<\/jats:italic>\n                      <\/jats:sup>\n                      pups similarly increased plasma inflammatory cytokines, PMA and PNA percentages, and NET formation. Compared to WT platelets,\n                      <jats:italic>P\u2010selectin<\/jats:italic>\n                      <jats:sup>\n                        <jats:italic>\u2212\/\u2212<\/jats:italic>\n                      <\/jats:sup>\n                      platelets induced similar elevations in plasma cytokines, but NET formation was attenuated and PMA and PNA percentages were comparable to those of sham\u2010transfused pups.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Discussion<\/jats:title>\n                    <jats:p>In a murine model of neonatal thrombocytopenia, transfusion of adult platelets increased PMA and PNA percentages, plasma inflammatory cytokines, and NET formation through both P\u2010selectin\u2010dependent and \u2010independent mechanisms. These effects may contribute to the negative outcomes seen with liberal neonatal platelet transfusion practices.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/trf.70042","type":"journal-article","created":{"date-parts":[[2025,12,16]],"date-time":"2025-12-16T14:19:26Z","timestamp":1765894766000},"page":"315-325","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Transfusion of adult platelets triggers inflammatory responses in newborn mice through both P\u2010selectin\u2010dependent and \u2010independent mechanisms"],"prefix":"10.1111","volume":"66","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1230-504X","authenticated-orcid":false,"given":"Patricia","family":"Davenport","sequence":"first","affiliation":[{"name":"Division of Newborn Medicine Boston Children's Hospital  Boston Massachusetts USA"},{"name":"Department of Pediatrics Harvard Medical School  Boston Massachusetts 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