{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T15:04:30Z","timestamp":1789657470415,"version":"4.0.1"},"reference-count":13,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2026,2,2]],"date-time":"2026-02-02T00:00:00Z","timestamp":1769990400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2026,2,2]],"date-time":"2026-02-02T00:00:00Z","timestamp":1769990400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Transfusion"],"published-print":{"date-parts":[[2026,4]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Heparin combined with sodium citrate has been used in leukocytapheresis for pediatric patients. Since 2022, we have performed leukocytapheresis using a highly concentrated sodium citrate solution (HSC, 5.32%) instead of acid citrate dextrose solution A (ACD\u2010A). We conducted this study to determine whether HSC use reduces run time and the total amount of anticoagulant solution in children.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Study Design and Methods<\/jats:title>\n                    <jats:p>We retrospectively analyzed data from consecutive autologous peripheral blood stem cell harvests (auto\u2010PBSCHs) between June 2012 and May 2025, including patient characteristics, mobilization methods, protocol used, anticoagulant type, run time, total anticoagulant solution volume, and collection efficiency.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Auto\u2010PBSCH was performed using the mononuclear cell collection (MNC) protocol in 28 procedures and the continuous MNC protocol in 20 procedures. ACD\u2010A was used in 35 procedures and HSC in 13. The run time was significantly shorter (204 [range, 117\u2013302] vs. 157\u2009min [range, 103\u2013227],\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u2009.02) in the HSC group and also confirmed in multivariable regression analysis (coefficient, \u221255.6; 95% confidence interval, \u2212106.2 to \u22125.04;\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u2009.03). In a subgroup analysis of cMNC procedures, CD34\n                      <jats:sup>+<\/jats:sup>\n                      collection efficiency showed a strong negative correlation with the proportion of run time devoted to establishing the initial interface (\n                      <jats:italic>r<\/jats:italic>\n                      \u2009=\u2009\u2212.73,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u2009.0003).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Delays in establishing the initial interface can reduce the duration of the effective MNC collection phase and may negatively affect collection efficiency. Careful attention to the initial interface phase is therefore warranted when using HSC.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/trf.70102","type":"journal-article","created":{"date-parts":[[2026,2,3]],"date-time":"2026-02-03T05:56:22Z","timestamp":1770098182000},"page":"677-688","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Pediatric autologous peripheral blood stem cell collection without heparin using a highly concentrated sodium citrate anticoagulant: A retrospective comparison with standard\n                    <scp>ACD<\/scp>\n                    \u2010A"],"prefix":"10.1111","volume":"66","author":[{"ORCID":"https:\/\/orcid.org\/0009-0005-7614-2448","authenticated-orcid":false,"given":"Keiko","family":"Fujii","sequence":"first","affiliation":[{"name":"Department of Hematology Okayama University Hospital  Okayama Japan"},{"name":"Division of Clinical Laboratory Okayama University Hospital  Okayama Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8021-4732","authenticated-orcid":false,"given":"Wataru","family":"Kitamura","sequence":"additional","affiliation":[{"name":"Department of Hematology Okayama University Hospital  Okayama Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kana","family":"Washio","sequence":"additional","affiliation":[{"name":"Department of Pediatrics Okayama University Hospital  Okayama Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kazuhiro","family":"Ikeuchi","sequence":"additional","affiliation":[{"name":"Department of Hematology Okayama University Hospital  Okayama Japan"},{"name":"Division of Transfusion and Cell Therapy Okayama University Hospital  Okayama Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joji","family":"Shimono","sequence":"additional","affiliation":[{"name":"Department of Hematology Okayama University Hospital  Okayama Japan"},{"name":"Division of Transfusion and Cell Therapy Okayama University Hospital  Okayama Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroyuki","family":"Murakami","sequence":"additional","affiliation":[{"name":"Department of Hematology Okayama University Hospital  Okayama Japan"},{"name":"Division of Transfusion and Cell Therapy Okayama University Hospital  Okayama Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7014-9095","authenticated-orcid":false,"given":"Fumio","family":"Otsuka","sequence":"additional","affiliation":[{"name":"Division of Clinical Laboratory Okayama University Hospital  Okayama Japan"},{"name":"Department of General Medicine Okayama University, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences  Okayama Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshinobu","family":"Maeda","sequence":"additional","affiliation":[{"name":"Department of Hematology Okayama University Hospital  Okayama Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8043-7768","authenticated-orcid":false,"given":"Nobuharu","family":"Fujii","sequence":"additional","affiliation":[{"name":"Department of Hematology Okayama University Hospital  Okayama Japan"},{"name":"Division of Transfusion and Cell Therapy Okayama University Hospital  Okayama 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