{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T15:06:00Z","timestamp":1789657560182,"version":"4.0.1"},"reference-count":38,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2026,2,6]],"date-time":"2026-02-06T00:00:00Z","timestamp":1770336000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,2,6]],"date-time":"2026-02-06T00:00:00Z","timestamp":1770336000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Transfusion"],"published-print":{"date-parts":[[2026,4]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\n                      Transfusion\u2010related acute lung injury (\n                      <jats:styled-content style=\"fixed-case\">TRALI<\/jats:styled-content>\n                      ) is a clinical condition characterized by acute non\u2010cardiogenic pulmonary edema during or after transfusion. Despite several mitigation strategies,\n                      <jats:styled-content style=\"fixed-case\">TRALI<\/jats:styled-content>\n                      remains a leading cause of transfusion\u2010related deaths. Tests on blood donors involved in\n                      <jats:styled-content style=\"fixed-case\">TRALI<\/jats:styled-content>\n                      , apart from leukocyte\/endothelial reactive alloantibodies, can also reveal the presence of neutrophil autoantibodies. Here, we ask the question whether these autoantibodies could play a role in the development of\n                      <jats:styled-content style=\"fixed-case\">TRALI<\/jats:styled-content>\n                      .\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials and methods<\/jats:title>\n                    <jats:p>\n                      Sera (\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u200915) from donors involved in TRALI and not containing alloantibodies against human neutrophil antigens (HNAs) and human leukocyte antigens (HLAs) were collected if their serological characterization was suggestive for the presence of neutrophil\u2010reactive autoantibodies. Sera (\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u200912) were suitable for the study. Their ability to bind complement, to activate neutrophils, and to disrupt the endothelial barrier using albumin influx through an endothelial monolayer in a transwell chamber in the presence and absence of neutrophils was investigated.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>None of the AIN sera, but 2\/12 TRALI sera, induced reactive oxygen species (ROS) in neutrophils. Both of these TRALI sera induced endothelial barrier permeability in the presence, but not in the absence, of neutrophils. These two sera did not activate complement.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Autoantibodies against neutrophils present in transfused blood components appear to be capable of contributing to TRALI in selected but not in all investigated cases, based on their ability to activate neutrophil ROS and induce endothelial cell permeability in vitro. Further analysis is required to understand the potential functional effects of neutrophil autoantibodies in TRALI.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/trf.70125","type":"journal-article","created":{"date-parts":[[2026,2,7]],"date-time":"2026-02-07T06:49:23Z","timestamp":1770446963000},"page":"794-802","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Neutrophil\u2010reactive autoantibodies in blood donors: Can we expect\n                    <scp>TRALI<\/scp>\n                    ?"],"prefix":"10.1111","volume":"66","author":[{"given":"Stefanie","family":"Jehle","sequence":"first","affiliation":[{"name":"Institute for Clinical Immunology, Transfusion Medicine, and Haemostaseology Justus\u2010Liebig\u2010University  Giessen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Darvin","family":"Preuss","sequence":"additional","affiliation":[{"name":"Institute for Clinical Immunology, Transfusion Medicine, and Haemostaseology Justus\u2010Liebig\u2010University  Giessen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yannick","family":"Waxmann","sequence":"additional","affiliation":[{"name":"Institute for Clinical Immunology, Transfusion Medicine, and Haemostaseology Justus\u2010Liebig\u2010University  Giessen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anja","family":"Spies\u2010Naumann","sequence":"additional","affiliation":[{"name":"Institute for Clinical Immunology, Transfusion Medicine, and Haemostaseology Justus\u2010Liebig\u2010University  Giessen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Silke","family":"Schmidt","sequence":"additional","affiliation":[{"name":"Institute for Clinical Immunology, Transfusion Medicine, and Haemostaseology Justus\u2010Liebig\u2010University  Giessen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1608-876X","authenticated-orcid":false,"given":"Rick","family":"Kapur","sequence":"additional","affiliation":[{"name":"Sanquin Blood Supply Foundation Department Research, Amsterdam UMC, Location University of Amsterdam, Landsteiner Laboratory  Amsterdam the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ulrich J.","family":"Sachs","sequence":"additional","affiliation":[{"name":"Institute for Clinical Immunology, Transfusion Medicine, and Haemostaseology Justus\u2010Liebig\u2010University  Giessen Germany"},{"name":"Center for Transfusion Medicine and Haemotherapy, Incorporating the Department of Thrombosis and Haemostasis and the European Haemophilia Comprehensive Care Center Giessen and Marburg University Hospital  Giessen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Behnaz","family":"Bayat","sequence":"additional","affiliation":[{"name":"Institute for Clinical Immunology, Transfusion Medicine, and Haemostaseology Justus\u2010Liebig\u2010University  Giessen Germany"},{"name":"Center for Transfusion Medicine and Haemotherapy, Incorporating the Department of Thrombosis and Haemostasis and the European Haemophilia Comprehensive Care Center Giessen and Marburg University Hospital  Giessen 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